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Article: Simultaneous quantification of ginsenosides in American ginseng (Panax quinquefolium) root powder by visible/near-infrared reflectance spectroscopy

TitleSimultaneous quantification of ginsenosides in American ginseng (Panax quinquefolium) root powder by visible/near-infrared reflectance spectroscopy
Authors
KeywordsAmerican ginseng
Ginsenosides
HPLC
Panax quinquefolium
Quantification
Visible/NIR spectroscopy
Issue Date1999
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jafcau
Citation
Journal Of Agricultural And Food Chemistry, 1999, v. 47 n. 7, p. 2771-2775 How to Cite?
AbstractNear-infrared reflectance spectroscopy (NIRS) was examined as a possible alternative to high-performance liquid chromatography (HPLC) for the analysis of ginsenosides from American ginseng (Panax quinquefolium) root powder (n = 26). NIR spectra were collected over 400-2500 nm. For each sample and individual ginsenoside quantified by HPLC, spectral data were regressed against the chemical data to develop prediction equations. The spectral prediction equations produced high correlation coefficient (1-VR) values and low standard errors of cross validation (SECV) values for the determination of individual and total ginsenosides. The contents of individual ginsenosides, Rb 1, Rb 2, Rc, Rd, Re, Rg 1, Ro, m-Rb 1, m-Rb 2, m-Rc, m-Rd, and total ginsenosides (X ± SECV) were (1.29 ± 0.18)%, (0.273 ± 0.096)%, (0.298 ± 0.052)%, (0.091 ± 0.027)%, (1.015 ± 0.114)%, (0.116 ± 0.018)%, (0.25 ± 0.040)%, (0.776 ± 0.116)%, (0.197 ± 0.074)%, (0.239 ± 0.083)%, (0.143 ± 0.042)%, and (4.393 ± 0.283)%, respectively. The (1-VR) values of cross validation were 0.877, 0.872, 0.955, 0.834, 0.899, 0.919, 0.325, 0.849, 0.902, 0.877, 0.871, and 0.963, respectively. Results indicated that the NIRS method could be used for the analysis of the major ginsenosides, Rb 1, Re, and m-Rb 1, as well as the total ginsenosides in American ginseng.
Persistent Identifierhttp://hdl.handle.net/10722/68385
ISSN
2015 Impact Factor: 2.857
2015 SCImago Journal Rankings: 1.246
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorRen, Gen_HK
dc.contributor.authorChen, Fen_HK
dc.date.accessioned2010-09-06T06:04:09Z-
dc.date.available2010-09-06T06:04:09Z-
dc.date.issued1999en_HK
dc.identifier.citationJournal Of Agricultural And Food Chemistry, 1999, v. 47 n. 7, p. 2771-2775en_HK
dc.identifier.issn0021-8561en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68385-
dc.description.abstractNear-infrared reflectance spectroscopy (NIRS) was examined as a possible alternative to high-performance liquid chromatography (HPLC) for the analysis of ginsenosides from American ginseng (Panax quinquefolium) root powder (n = 26). NIR spectra were collected over 400-2500 nm. For each sample and individual ginsenoside quantified by HPLC, spectral data were regressed against the chemical data to develop prediction equations. The spectral prediction equations produced high correlation coefficient (1-VR) values and low standard errors of cross validation (SECV) values for the determination of individual and total ginsenosides. The contents of individual ginsenosides, Rb 1, Rb 2, Rc, Rd, Re, Rg 1, Ro, m-Rb 1, m-Rb 2, m-Rc, m-Rd, and total ginsenosides (X ± SECV) were (1.29 ± 0.18)%, (0.273 ± 0.096)%, (0.298 ± 0.052)%, (0.091 ± 0.027)%, (1.015 ± 0.114)%, (0.116 ± 0.018)%, (0.25 ± 0.040)%, (0.776 ± 0.116)%, (0.197 ± 0.074)%, (0.239 ± 0.083)%, (0.143 ± 0.042)%, and (4.393 ± 0.283)%, respectively. The (1-VR) values of cross validation were 0.877, 0.872, 0.955, 0.834, 0.899, 0.919, 0.325, 0.849, 0.902, 0.877, 0.871, and 0.963, respectively. Results indicated that the NIRS method could be used for the analysis of the major ginsenosides, Rb 1, Re, and m-Rb 1, as well as the total ginsenosides in American ginseng.en_HK
dc.languageengen_HK
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jafcauen_HK
dc.relation.ispartofJournal of Agricultural and Food Chemistryen_HK
dc.subjectAmerican ginsengen_HK
dc.subjectGinsenosidesen_HK
dc.subjectHPLCen_HK
dc.subjectPanax quinquefoliumen_HK
dc.subjectQuantificationen_HK
dc.subjectVisible/NIR spectroscopyen_HK
dc.titleSimultaneous quantification of ginsenosides in American ginseng (Panax quinquefolium) root powder by visible/near-infrared reflectance spectroscopyen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-8561&volume=47&spage=2771&epage=2775&date=1999&atitle=Simultaneous+quantification+of+ginsenosides+in+American+ginseng+(Panax+quinquefolium)+root+powder+by+visible/near+infrared+reflectance+spectroscopyen_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/jf9812477en_HK
dc.identifier.pmid10552563en_HK
dc.identifier.scopuseid_2-s2.0-0032819227en_HK
dc.identifier.hkuros53005en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032819227&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume47en_HK
dc.identifier.issue7en_HK
dc.identifier.spage2771en_HK
dc.identifier.epage2775en_HK
dc.identifier.isiWOS:000081556400049-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridRen, G=36681498500en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK

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