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Article: Determination of squalene using high-performance liquid chromatography with diode array detection

TitleDetermination of squalene using high-performance liquid chromatography with diode array detection
Authors
KeywordsColumn liquid chromatography
Extraction and fractional crystallization
Squalene from natural and pharmaceutical sources
Issue Date2004
PublisherFriedr Vieweg und Sohn Verlagsgesellschaft mbH. The Journal's web site is located at http://www.elsevier.com/locate/chromatographia
Citation
Chromatographia, 2004, v. 59 n. 5-6, p. 367-371 How to Cite?
AbstractA high-performance liquid chromotographic method with photodiode array detection has been developed for the determination of squalene. After treated by extraction and fractional crystallization, squalene was analyzed on a C 18 column (150 x 3.9 mm, 5 μm) with acetonitrile as mobile phase. Excellent linearity of the calibration curve was observed in the range of 100-40000 μgL-1 and the detection limit was 40 μgL -1. The recoveries were from 89.6% to 100.5% and the relative standard deviations were from 0.5% to 1.4%. The method was successfully applied to the determination of squalene in squalene capsules, olive oil, algal lipids and algal cells.
Persistent Identifierhttp://hdl.handle.net/10722/68383
ISSN
2015 Impact Factor: 1.332
2015 SCImago Journal Rankings: 0.480
References

 

DC FieldValueLanguage
dc.contributor.authorLu, HTen_HK
dc.contributor.authorJiang, Yen_HK
dc.contributor.authorChen, Fen_HK
dc.date.accessioned2010-09-06T06:04:08Z-
dc.date.available2010-09-06T06:04:08Z-
dc.date.issued2004en_HK
dc.identifier.citationChromatographia, 2004, v. 59 n. 5-6, p. 367-371en_HK
dc.identifier.issn0009-5893en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68383-
dc.description.abstractA high-performance liquid chromotographic method with photodiode array detection has been developed for the determination of squalene. After treated by extraction and fractional crystallization, squalene was analyzed on a C 18 column (150 x 3.9 mm, 5 μm) with acetonitrile as mobile phase. Excellent linearity of the calibration curve was observed in the range of 100-40000 μgL-1 and the detection limit was 40 μgL -1. The recoveries were from 89.6% to 100.5% and the relative standard deviations were from 0.5% to 1.4%. The method was successfully applied to the determination of squalene in squalene capsules, olive oil, algal lipids and algal cells.en_HK
dc.languageengen_HK
dc.publisherFriedr Vieweg und Sohn Verlagsgesellschaft mbH. The Journal's web site is located at http://www.elsevier.com/locate/chromatographiaen_HK
dc.relation.ispartofChromatographiaen_HK
dc.subjectColumn liquid chromatographyen_HK
dc.subjectExtraction and fractional crystallizationen_HK
dc.subjectSqualene from natural and pharmaceutical sourcesen_HK
dc.titleDetermination of squalene using high-performance liquid chromatography with diode array detectionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0009-5893&volume=59&spage=367&epage=371&date=2004&atitle=Determination+of+squalene+using+high-performance+liquid+chromatography+with+diode+array+detectionen_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-1642443922en_HK
dc.identifier.hkuros90886en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-1642443922&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume59en_HK
dc.identifier.issue5-6en_HK
dc.identifier.spage367en_HK
dc.identifier.epage371en_HK
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridLu, HT=7404843255en_HK
dc.identifier.scopusauthoridJiang, Y=24605346600en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK

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