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Article: Separation and Analysis of Carotenoids and Chlorophylls in Haematococcus lacustris by High-Performance Liquid Chromatography Photodiode Array Detection

TitleSeparation and Analysis of Carotenoids and Chlorophylls in Haematococcus lacustris by High-Performance Liquid Chromatography Photodiode Array Detection
Authors
KeywordsAstaxanthin esters
Carotenoids
Chlorophylls
Haematococcus lacustris
HPLC
Issue Date1997
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jafcau
Citation
Journal Of Agricultural And Food Chemistry, 1997, v. 45 n. 5, p. 1952-1956 How to Cite?
AbstractAn improved HPLC method was developed for the separation and analysis of carotenoids and chlorophylls in a green alga, Haematococcus lacustris. Six astaxanthin esters, the principal carotenoids in H. lacustris, were separated and indicated as astaxanthin esters 1-6. The retention times of the six astaxanthin esters were 11.8, 14.3, 15.0, 18.2, 22.0, and 28.4 min, respectively. Astaxanthin ester 4 was the main astaxanthin ester, which accounted for 44-49% of the total astaxanthin esters. The six astaxanthin esters had the same absorption spectra, and their maximum absorption wavelengths in the mobile phase were all 481.3 nm. Relatively small amounts of β-carotene, canthaxanthin, and echinenone were detected from the alga extracts compared to astaxanthin esters, lutein, and chlorophylls. During the cultivation, the light intensity for illumination of the culture was increased from 90 to 190 μmol/(m2 s) to investigate its effect on pigment formation. The content of the total astaxanthin esters was higher with enhanced light intensity after 4 days of cultivation (6.8 mg/g of dry weight) than after 8 days of cultivation (5.0 mg/g of dry weight). This HPLC method could be used to separate and determine the various algal pigments and assist in the study of biosynthesis of ketocarotenoids in algae.
Persistent Identifierhttp://hdl.handle.net/10722/68376
ISSN
2015 Impact Factor: 2.857
2015 SCImago Journal Rankings: 1.246
References

 

DC FieldValueLanguage
dc.contributor.authorYuan, JPen_HK
dc.contributor.authorGong, XDen_HK
dc.contributor.authorChen, Fen_HK
dc.date.accessioned2010-09-06T06:04:04Z-
dc.date.available2010-09-06T06:04:04Z-
dc.date.issued1997en_HK
dc.identifier.citationJournal Of Agricultural And Food Chemistry, 1997, v. 45 n. 5, p. 1952-1956en_HK
dc.identifier.issn0021-8561en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68376-
dc.description.abstractAn improved HPLC method was developed for the separation and analysis of carotenoids and chlorophylls in a green alga, Haematococcus lacustris. Six astaxanthin esters, the principal carotenoids in H. lacustris, were separated and indicated as astaxanthin esters 1-6. The retention times of the six astaxanthin esters were 11.8, 14.3, 15.0, 18.2, 22.0, and 28.4 min, respectively. Astaxanthin ester 4 was the main astaxanthin ester, which accounted for 44-49% of the total astaxanthin esters. The six astaxanthin esters had the same absorption spectra, and their maximum absorption wavelengths in the mobile phase were all 481.3 nm. Relatively small amounts of β-carotene, canthaxanthin, and echinenone were detected from the alga extracts compared to astaxanthin esters, lutein, and chlorophylls. During the cultivation, the light intensity for illumination of the culture was increased from 90 to 190 μmol/(m2 s) to investigate its effect on pigment formation. The content of the total astaxanthin esters was higher with enhanced light intensity after 4 days of cultivation (6.8 mg/g of dry weight) than after 8 days of cultivation (5.0 mg/g of dry weight). This HPLC method could be used to separate and determine the various algal pigments and assist in the study of biosynthesis of ketocarotenoids in algae.en_HK
dc.languageengen_HK
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jafcauen_HK
dc.relation.ispartofJournal of Agricultural and Food Chemistryen_HK
dc.subjectAstaxanthin estersen_HK
dc.subjectCarotenoidsen_HK
dc.subjectChlorophyllsen_HK
dc.subjectHaematococcus lacustrisen_HK
dc.subjectHPLCen_HK
dc.titleSeparation and Analysis of Carotenoids and Chlorophylls in Haematococcus lacustris by High-Performance Liquid Chromatography Photodiode Array Detectionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-8561&volume=45&issue=5&spage=1952&epage=1956&date=1997&atitle=Separation+and+analysis+of+carotenoids+and+chlorophylls+in+Haematococcus+lacustris+by+High-Performance+Liquid+Chromatography+photodiode+array+detectionen_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-0001534170en_HK
dc.identifier.hkuros23372en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0001534170&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume45en_HK
dc.identifier.issue5en_HK
dc.identifier.spage1952en_HK
dc.identifier.epage1956en_HK
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridYuan, JP=7403401467en_HK
dc.identifier.scopusauthoridGong, XD=7201999182en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK

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