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- Publisher Website: 10.1016/S0021-9673(01)01022-6
- Scopus: eid_2-s2.0-0035800701
- PMID: 11519799
- WOS: WOS:000170432100013
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Article: Preparative isolation and purification of astaxanthin from the microalga Chlorococcum sp. by high-speed counter-current chromatography
Title | Preparative isolation and purification of astaxanthin from the microalga Chlorococcum sp. by high-speed counter-current chromatography |
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Authors | |
Keywords | Antioxidants Astaxanthin Carotenoids Chlorococcum sp. Counter-current chromatography |
Issue Date | 2001 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chroma |
Citation | Journal Of Chromatography A, 2001, v. 925 n. 1-2, p. 133-137 How to Cite? |
Abstract | High-speed counter-current chromatography was applied to the isolation and purification of astaxanthin from microalgae. The crude astaxanthin was obtained by extraction with organic solvents after the astaxanthin esters were saponified. Preparative high-speed counter-current chromatography with a two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-water (5:5:6.5:3, v/v) was successfully performed yielding astaxanthin at 97% purity from 250 mg of the crude extract in a one-step separation. Copyright © 2001 Elsevier Science B.V. |
Persistent Identifier | http://hdl.handle.net/10722/68367 |
ISSN | 2023 Impact Factor: 3.8 2023 SCImago Journal Rankings: 0.717 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Li, HB | en_HK |
dc.contributor.author | Chen, F | en_HK |
dc.date.accessioned | 2010-09-06T06:03:59Z | - |
dc.date.available | 2010-09-06T06:03:59Z | - |
dc.date.issued | 2001 | en_HK |
dc.identifier.citation | Journal Of Chromatography A, 2001, v. 925 n. 1-2, p. 133-137 | en_HK |
dc.identifier.issn | 0021-9673 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/68367 | - |
dc.description.abstract | High-speed counter-current chromatography was applied to the isolation and purification of astaxanthin from microalgae. The crude astaxanthin was obtained by extraction with organic solvents after the astaxanthin esters were saponified. Preparative high-speed counter-current chromatography with a two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-water (5:5:6.5:3, v/v) was successfully performed yielding astaxanthin at 97% purity from 250 mg of the crude extract in a one-step separation. Copyright © 2001 Elsevier Science B.V. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chroma | en_HK |
dc.relation.ispartof | Journal of Chromatography A | en_HK |
dc.rights | Journal of Chromatography A. Copyright © Elsevier BV. | en_HK |
dc.subject | Antioxidants | en_HK |
dc.subject | Astaxanthin | en_HK |
dc.subject | Carotenoids | en_HK |
dc.subject | Chlorococcum sp. | en_HK |
dc.subject | Counter-current chromatography | en_HK |
dc.title | Preparative isolation and purification of astaxanthin from the microalga Chlorococcum sp. by high-speed counter-current chromatography | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9673&volume=925&spage=133&epage=137&date=2001&atitle=Preparative+isolation+and+purification+of+astaxanthin+from+the+microalga+Chlorococcum+sp.+by+high-speed+counter-current+chromatography | en_HK |
dc.identifier.email | Chen, F: sfchen@hku.hk | en_HK |
dc.identifier.authority | Chen, F=rp00672 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/S0021-9673(01)01022-6 | en_HK |
dc.identifier.pmid | 11519799 | - |
dc.identifier.scopus | eid_2-s2.0-0035800701 | en_HK |
dc.identifier.hkuros | 72676 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0035800701&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 925 | en_HK |
dc.identifier.issue | 1-2 | en_HK |
dc.identifier.spage | 133 | en_HK |
dc.identifier.epage | 137 | en_HK |
dc.identifier.isi | WOS:000170432100013 | - |
dc.publisher.place | Netherlands | en_HK |
dc.identifier.scopusauthorid | Li, HB=25649944400 | en_HK |
dc.identifier.scopusauthorid | Chen, F=7404907980 | en_HK |
dc.identifier.issnl | 0021-9673 | - |