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Article: Inhibition of endogenous trypsin- and chymotrypsin-like activities in transgenic lettuce expressing heterogeneous proteinase inhibitor SaPIN2a

TitleInhibition of endogenous trypsin- and chymotrypsin-like activities in transgenic lettuce expressing heterogeneous proteinase inhibitor SaPIN2a
Authors
KeywordsChymotrypsin
Lactuca
Protease
Proteolysis
Solanum
Trypsin
Issue Date2004
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00425
Citation
Planta, 2004, v. 218 n. 4, p. 623-629 How to Cite?
AbstractSaPIN2a, a proteinase inhibitor II from American black nightshade (Solanum americanum Mill.) is highly expressed in the phloem and could be involved in regulating proteolysis in the sieve elements. To further investigate the physiological role of SaPIN2a, we have produced transgenic lettuce (Lactuca sativa L.) expressing SaPIN2a from the CaMV35S promoter by Agrobacterium- mediated transformation. Stable integration of the SaPIN2a cDNA and its inheritance in transgenic lines were confirmed by Southern blot analysis and segregation analysis of the R1 progeny. SaPIN2a mRNA was detected in both the R0 and R1 transformants on northern blot analysis but the SaPIN2a protein was not detected on western blot analysis using anti-peptide antibodies against SaPIN2a. Despite an absence of significant inhibitory activity against bovine trypsin and chymotrypsin in extracts of transgenic lettuce, the endogenous trypsin-like activity in each transgenic line was almost completely inhibited, and the endogenous chymotrypsin-like activity moderately inhibited. Our finding that heterogeneously expressed SaPIN2a in transgenic lettuce inhibits plant endogenous protease activity further indicates that SaPIN2a regulates proteolysis, and could be potentially exploited for the protection of foreign protein production in transgenic plants.
Persistent Identifierhttp://hdl.handle.net/10722/68354
ISSN
2015 Impact Factor: 3.239
2015 SCImago Journal Rankings: 1.470
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorXu, ZFen_HK
dc.contributor.authorTeng, WLen_HK
dc.contributor.authorChye, MLen_HK
dc.date.accessioned2010-09-06T06:03:51Z-
dc.date.available2010-09-06T06:03:51Z-
dc.date.issued2004en_HK
dc.identifier.citationPlanta, 2004, v. 218 n. 4, p. 623-629en_HK
dc.identifier.issn0032-0935en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68354-
dc.description.abstractSaPIN2a, a proteinase inhibitor II from American black nightshade (Solanum americanum Mill.) is highly expressed in the phloem and could be involved in regulating proteolysis in the sieve elements. To further investigate the physiological role of SaPIN2a, we have produced transgenic lettuce (Lactuca sativa L.) expressing SaPIN2a from the CaMV35S promoter by Agrobacterium- mediated transformation. Stable integration of the SaPIN2a cDNA and its inheritance in transgenic lines were confirmed by Southern blot analysis and segregation analysis of the R1 progeny. SaPIN2a mRNA was detected in both the R0 and R1 transformants on northern blot analysis but the SaPIN2a protein was not detected on western blot analysis using anti-peptide antibodies against SaPIN2a. Despite an absence of significant inhibitory activity against bovine trypsin and chymotrypsin in extracts of transgenic lettuce, the endogenous trypsin-like activity in each transgenic line was almost completely inhibited, and the endogenous chymotrypsin-like activity moderately inhibited. Our finding that heterogeneously expressed SaPIN2a in transgenic lettuce inhibits plant endogenous protease activity further indicates that SaPIN2a regulates proteolysis, and could be potentially exploited for the protection of foreign protein production in transgenic plants.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00425en_HK
dc.relation.ispartofPlantaen_HK
dc.subjectChymotrypsinen_HK
dc.subjectLactucaen_HK
dc.subjectProteaseen_HK
dc.subjectProteolysisen_HK
dc.subjectSolanumen_HK
dc.subjectTrypsinen_HK
dc.titleInhibition of endogenous trypsin- and chymotrypsin-like activities in transgenic lettuce expressing heterogeneous proteinase inhibitor SaPIN2aen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0032-0935&volume=218&spage=623&epage=629&date=2004&atitle=Inhibition+of+endogenous+trypsin-+and+chymotrypsin-like+activities+in+transgenic+lettuce+expressing+heterogeneous+proteinase+inhibitor+SaPIN2aen_HK
dc.identifier.emailChye, ML: mlchye@hkucc.hku.hken_HK
dc.identifier.authorityChye, ML=rp00687en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00425-003-1138-9en_HK
dc.identifier.pmid14574575en_HK
dc.identifier.scopuseid_2-s2.0-1442291008en_HK
dc.identifier.hkuros92091en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-1442291008&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume218en_HK
dc.identifier.issue4en_HK
dc.identifier.spage623en_HK
dc.identifier.epage629en_HK
dc.identifier.isiWOS:000188933000014-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridXu, ZF=7405425506en_HK
dc.identifier.scopusauthoridTeng, WL=7004808953en_HK
dc.identifier.scopusauthoridChye, ML=7003905460en_HK

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