File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Microsatellites in starch-synthesizing genes in relation to starch physicochemical properties in waxy rice (Oryza sativa L.)

TitleMicrosatellites in starch-synthesizing genes in relation to starch physicochemical properties in waxy rice (Oryza sativa L.)
Authors
KeywordsMicrosatellite
Rice
Soluble starch synthase
Starch branching enzyme
Starch quality
Wx
Issue Date2002
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00122/index.htm
Citation
Theoretical And Applied Genetics, 2002, v. 105 n. 6-7, p. 898-905 How to Cite?
AbstractRice starch is composed of amylose and amylopectin. Amylose content, an important determinant of rice starch quality, is primarily controlled by the waxy gene, encoding granule-bound starch synthase (GBSS). The starch branching enzyme (SBE) and soluble starch synthase (SSS) play major roles in the synthesis of amylopectin. Microsatellite polymorphisms in the three genes, the wx gene encoding granule-bound starch synthase I, the SBE gene encoding starch branching enzyme I and the SSS gene encoding soluble starch synthase I, were studied for 56 accessions of waxy rice (Oryza sativa L.). Four (CT)n microsatellite alleles, (CT)16, (CT)17, (CT)18 and (CT)19, at the wx locus were detected in this set of waxy rice, of which (CT)17 was the most frequent. Three (CT)n microsatellite allele classes were found at the SBE locus, (CT)8 or (CT)10 together with an insertion sequence of CTCTCGGGCGA, and (CT)8 alone without the insertion. There were multiple microsatellites clustered at the SSS locus. However, these alleles can also be grouped into three classes, i.e. the allele class SSS-A = (AC)2...TCC(TC)11...(TC)5C(ACC) 11, the allele class SSS-B = (AC)3...TCT(TC)6...(TC)4C(ACC)9, and the allele class SSS-C = (AC)3...TCT(TC)6...(TC)4 C(ACC)8. The analyses of starch physicochemical properties among different microsatellite genotypes indicated that the waxy rice group with the (CT)19 allele, the SBE-A allele and the SSS-B allele was quite different from other groups. Nine out of 15 accessions with a high gelatinization temperature (GT) belonged to the wx (CT)19 group, all of them belonged to the SBE-A group and 13 of them belonged to the SSS-B group. These microsatellites might be useful in marker-assisted breeding for the improvement of rice grain quality.
Persistent Identifierhttp://hdl.handle.net/10722/68352
ISSN
2015 Impact Factor: 3.9
2015 SCImago Journal Rankings: 1.930
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorBao, JSen_HK
dc.contributor.authorCorke, Hen_HK
dc.contributor.authorSun, Men_HK
dc.date.accessioned2010-09-06T06:03:50Z-
dc.date.available2010-09-06T06:03:50Z-
dc.date.issued2002en_HK
dc.identifier.citationTheoretical And Applied Genetics, 2002, v. 105 n. 6-7, p. 898-905en_HK
dc.identifier.issn0040-5752en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68352-
dc.description.abstractRice starch is composed of amylose and amylopectin. Amylose content, an important determinant of rice starch quality, is primarily controlled by the waxy gene, encoding granule-bound starch synthase (GBSS). The starch branching enzyme (SBE) and soluble starch synthase (SSS) play major roles in the synthesis of amylopectin. Microsatellite polymorphisms in the three genes, the wx gene encoding granule-bound starch synthase I, the SBE gene encoding starch branching enzyme I and the SSS gene encoding soluble starch synthase I, were studied for 56 accessions of waxy rice (Oryza sativa L.). Four (CT)n microsatellite alleles, (CT)16, (CT)17, (CT)18 and (CT)19, at the wx locus were detected in this set of waxy rice, of which (CT)17 was the most frequent. Three (CT)n microsatellite allele classes were found at the SBE locus, (CT)8 or (CT)10 together with an insertion sequence of CTCTCGGGCGA, and (CT)8 alone without the insertion. There were multiple microsatellites clustered at the SSS locus. However, these alleles can also be grouped into three classes, i.e. the allele class SSS-A = (AC)2...TCC(TC)11...(TC)5C(ACC) 11, the allele class SSS-B = (AC)3...TCT(TC)6...(TC)4C(ACC)9, and the allele class SSS-C = (AC)3...TCT(TC)6...(TC)4 C(ACC)8. The analyses of starch physicochemical properties among different microsatellite genotypes indicated that the waxy rice group with the (CT)19 allele, the SBE-A allele and the SSS-B allele was quite different from other groups. Nine out of 15 accessions with a high gelatinization temperature (GT) belonged to the wx (CT)19 group, all of them belonged to the SBE-A group and 13 of them belonged to the SSS-B group. These microsatellites might be useful in marker-assisted breeding for the improvement of rice grain quality.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00122/index.htmen_HK
dc.relation.ispartofTheoretical and Applied Geneticsen_HK
dc.subjectMicrosatelliteen_HK
dc.subjectRiceen_HK
dc.subjectSoluble starch synthaseen_HK
dc.subjectStarch branching enzymeen_HK
dc.subjectStarch qualityen_HK
dc.subjectWxen_HK
dc.titleMicrosatellites in starch-synthesizing genes in relation to starch physicochemical properties in waxy rice (Oryza sativa L.)en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0040-5752&volume=105&issue=6-7&spage=898&epage=905&date=2002&atitle=Microsatellites+in+starch-synthesizing+genes+in+relation+to+starch+physicochemical+properties+in+waxy+rice+(Oryza+sativa+L.)en_HK
dc.identifier.emailCorke, H: harold@hku.hken_HK
dc.identifier.emailSun, M: meisun@hkucc.hku.hken_HK
dc.identifier.authorityCorke, H=rp00688en_HK
dc.identifier.authoritySun, M=rp00779en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00122-002-1049-3en_HK
dc.identifier.scopuseid_2-s2.0-0036944922en_HK
dc.identifier.hkuros76119en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036944922&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume105en_HK
dc.identifier.issue6-7en_HK
dc.identifier.spage898en_HK
dc.identifier.epage905en_HK
dc.identifier.isiWOS:000179685700013-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridBao, JS=7201398486en_HK
dc.identifier.scopusauthoridCorke, H=7007102942en_HK
dc.identifier.scopusauthoridSun, M=7403181447en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats