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Article: Heterotrophic production of biomass and lutein by Chlorella protothecoides on various nitrogen sources

TitleHeterotrophic production of biomass and lutein by Chlorella protothecoides on various nitrogen sources
Authors
KeywordsChlorella protothecoides
Heterotrophic culture
Kinetic model
Lutein
Nitrogen
Issue Date2000
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/enzmictec
Citation
Enzyme And Microbial Technology, 2000, v. 27 n. 3-5, p. 312-318 How to Cite?
AbstractThe effects of nitrate, ammonium, and urea as nitrogen sources on the heterotrophic growth of Chlorella protothecoides were investigated using flask cultures. No appreciable inhibitory effect on the algal growth was observed over a nitrogen concentration range of 0.85-1.7 g l -1. In contrast, differences in specific growth rate and biomass production were found among the cultures with the various nitrogen compounds. The influence of different nitrogen sources at a concentration equivalent to 1.7 g l -1 nitrogen on the heterotrophic production of biomass and lutein by C. protothecoides was investigated using the culture medium containing 40 g l -1 glucose as the sole carbon and energy source in fermentors. The maximum biomass concentrations in the three cultures with nitrate, ammonium, and urea were 18.4, 18.9, and 19.6 g l -1 dry cells, respectively. The maximum lutein yields in these cultures were between 68.42 and 83.81 mg l -1. The highest yields of both biomass and lutein were achieved in the culture with urea. It was therefore concluded that urea was the best nitrogen source for the production of biomass and lutein. Based on the experimental results, a group of kinetic models describing cell growth, lutein production, and glucose and nitrogen consumption were proposed and a satisfactory fit was found between the experimental results and predicted values. Dynamic analysis of models demonstrated that enhancing initial nitrogen concentration in fermentor cultures, which correspondingly enhances cell growth and lutein formation, may shorten the fermentation cycle by 25-46%. Copyright (C) 2000 Elsevier Science Inc. | The effects of nitrate, ammonium, and urea as nitrogen sources on the heterotrophic growth of Chlorella protothecoides were investigated using flask cultures. No appreciable inhibitory effect on the algal growth was observed over a nitrogen concentration range of 0.85-1.7 g l -1. In contrast, differences in specific growth rate and biomass production were found among the cultures with the various nitrogen compounds. The influence of different nitrogen sources at a concentration equivalent to 1.7 g l -1 nitrogen on the heterotrophic production of biomass and lutein by C. protothecoides was investigated using the culture medium containing 40 g l -1 glucose as the sole carbon and energy source in fermentors. The maximum biomass concentrations in the three cultures with nitrate, ammonium, and urea were 18.4, 18.9, and 19.6 g l -1 dry cells, respectively. The maximum lutein yields in these cultures were between 68.42 and 83.81 mg l -1. The highest yields of both biomass and lutein were achieved in the culture with urea. It was therefore concluded that urea was the best nitrogen source for the production of biomass and lutein. Based on the experimental results, a group of kinetic models describing cell growth, lutein production, and glucose and nitrogen consumption were proposed and a satisfactory fit was found between the experimental results and predicted values. Dynamic analysis of models demonstrated that enhancing initial nitrogen concentration in fermentor cultures, which correspondingly enhances cell growth and lutein formation, may shorten the fermentation cycle by 25-46%.
Persistent Identifierhttp://hdl.handle.net/10722/68344
ISSN
2015 Impact Factor: 2.624
2015 SCImago Journal Rankings: 0.854
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorShi, XMen_HK
dc.contributor.authorZhang, XWen_HK
dc.contributor.authorChen, Fen_HK
dc.date.accessioned2010-09-06T06:03:45Z-
dc.date.available2010-09-06T06:03:45Z-
dc.date.issued2000en_HK
dc.identifier.citationEnzyme And Microbial Technology, 2000, v. 27 n. 3-5, p. 312-318en_HK
dc.identifier.issn0141-0229en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68344-
dc.description.abstractThe effects of nitrate, ammonium, and urea as nitrogen sources on the heterotrophic growth of Chlorella protothecoides were investigated using flask cultures. No appreciable inhibitory effect on the algal growth was observed over a nitrogen concentration range of 0.85-1.7 g l -1. In contrast, differences in specific growth rate and biomass production were found among the cultures with the various nitrogen compounds. The influence of different nitrogen sources at a concentration equivalent to 1.7 g l -1 nitrogen on the heterotrophic production of biomass and lutein by C. protothecoides was investigated using the culture medium containing 40 g l -1 glucose as the sole carbon and energy source in fermentors. The maximum biomass concentrations in the three cultures with nitrate, ammonium, and urea were 18.4, 18.9, and 19.6 g l -1 dry cells, respectively. The maximum lutein yields in these cultures were between 68.42 and 83.81 mg l -1. The highest yields of both biomass and lutein were achieved in the culture with urea. It was therefore concluded that urea was the best nitrogen source for the production of biomass and lutein. Based on the experimental results, a group of kinetic models describing cell growth, lutein production, and glucose and nitrogen consumption were proposed and a satisfactory fit was found between the experimental results and predicted values. Dynamic analysis of models demonstrated that enhancing initial nitrogen concentration in fermentor cultures, which correspondingly enhances cell growth and lutein formation, may shorten the fermentation cycle by 25-46%. Copyright (C) 2000 Elsevier Science Inc. | The effects of nitrate, ammonium, and urea as nitrogen sources on the heterotrophic growth of Chlorella protothecoides were investigated using flask cultures. No appreciable inhibitory effect on the algal growth was observed over a nitrogen concentration range of 0.85-1.7 g l -1. In contrast, differences in specific growth rate and biomass production were found among the cultures with the various nitrogen compounds. The influence of different nitrogen sources at a concentration equivalent to 1.7 g l -1 nitrogen on the heterotrophic production of biomass and lutein by C. protothecoides was investigated using the culture medium containing 40 g l -1 glucose as the sole carbon and energy source in fermentors. The maximum biomass concentrations in the three cultures with nitrate, ammonium, and urea were 18.4, 18.9, and 19.6 g l -1 dry cells, respectively. The maximum lutein yields in these cultures were between 68.42 and 83.81 mg l -1. The highest yields of both biomass and lutein were achieved in the culture with urea. It was therefore concluded that urea was the best nitrogen source for the production of biomass and lutein. Based on the experimental results, a group of kinetic models describing cell growth, lutein production, and glucose and nitrogen consumption were proposed and a satisfactory fit was found between the experimental results and predicted values. Dynamic analysis of models demonstrated that enhancing initial nitrogen concentration in fermentor cultures, which correspondingly enhances cell growth and lutein formation, may shorten the fermentation cycle by 25-46%.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/enzmictecen_HK
dc.relation.ispartofEnzyme and Microbial Technologyen_HK
dc.rightsEnzyme and Microbial Technology. Copyright © Elsevier Inc.en_HK
dc.subjectChlorella protothecoidesen_HK
dc.subjectHeterotrophic cultureen_HK
dc.subjectKinetic modelen_HK
dc.subjectLuteinen_HK
dc.subjectNitrogenen_HK
dc.titleHeterotrophic production of biomass and lutein by Chlorella protothecoides on various nitrogen sourcesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0141-0229&volume=27&spage=312&epage=318&date=2000&atitle=Heterotrophic+production+of+biomass+and+lutein+by+Chlorella+protothecoides+on+various+nitrogen+sourcesen_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0141-0229(00)00208-8en_HK
dc.identifier.scopuseid_2-s2.0-0034255898en_HK
dc.identifier.hkuros52824en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034255898&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume27en_HK
dc.identifier.issue3-5en_HK
dc.identifier.spage312en_HK
dc.identifier.epage318en_HK
dc.identifier.isiWOS:000088241800016-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridShi, XM=7402953728en_HK
dc.identifier.scopusauthoridZhang, XW=16044335800en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK
dc.identifier.citeulike9577939-

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