File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Differential expression of NMDA and AMPA/KA receptor subunits in the inferior olive of postnatal rats

TitleDifferential expression of NMDA and AMPA/KA receptor subunits in the inferior olive of postnatal rats
Authors
KeywordsAMPA/KA receptor
Co-localization
Immunohistochemistry
Inferior olive
NMDA receptor
Issue Date2006
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainres
Citation
Brain Research, 2006, v. 1067 n. 1, p. 103-114 How to Cite?
AbstractWe have employed immunohistochemistry to determine the expression patterns of receptor subunits of N-methyl-d-aspartate (NMDA-NR1 and NR2A/B) and α-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid/kainic acid (AMPA/KA-GluR1, GluR2, GluR2/3, GluR4, and GluR5/6/7) in the inferior olive of postnatal rats up to adulthood. Immunoreactivity for distinct receptor subunits was predominantly localized in the soma and dendrites of neurons. Semi-quantification showed that the overall immunoreactivity in the inferior olive of adults was intense for GluR1, moderate for NR1 and NR2A/B, and low for GluR2, GluR2/3, GluR4, and GluR5/6/7. At P7, GluR1 was restricted to the dorsomedial cell column, subnucleus beta, principal nucleus and ventrolateral protrusion while the other subunits were found in all subnuclei of the inferior olive. The immunoreactivities for all glutamate receptor subunits ranged from low to moderate. As the rats matured, the immunoreactivity of GluR4 decreased after the second postnatal week, while those of the other subunits showed a general trend of increase, reaching adult level during the third postnatal week. Double immunofluorescence revealed that all NR1-containing neurons exhibited NR2A/B immunoreactivity, indicating that native NMDA receptors comprise of hetero-oligomeric combinations of NR1 and NR2A/B. Furthermore, co-localization of NMDA and AMPA/KA receptor subunits was demonstrated in individual neurons of the inferior olive. All NR1-containing neurons exhibited GluR1 immunoreactivity, and all NR2A/B-containing neurons showed GluR5/6/7 immunoreactivity. Our data suggest that NMDA and AMPA/KA receptors are involved in glutamate-mediated neurotransmission, contributing to synaptic plasticity and reorganization of circuitry in the inferior olive during postnatal development. © 2005 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/68332
ISSN
2015 Impact Factor: 2.561
2015 SCImago Journal Rankings: 1.351
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, LWen_HK
dc.contributor.authorTse, YCen_HK
dc.contributor.authorLi, Cen_HK
dc.contributor.authorGuan, ZLen_HK
dc.contributor.authorLai, CHen_HK
dc.contributor.authorYung, KKLen_HK
dc.contributor.authorShum, DKYen_HK
dc.contributor.authorChan, YSen_HK
dc.date.accessioned2010-09-06T06:03:34Z-
dc.date.available2010-09-06T06:03:34Z-
dc.date.issued2006en_HK
dc.identifier.citationBrain Research, 2006, v. 1067 n. 1, p. 103-114en_HK
dc.identifier.issn0006-8993en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68332-
dc.description.abstractWe have employed immunohistochemistry to determine the expression patterns of receptor subunits of N-methyl-d-aspartate (NMDA-NR1 and NR2A/B) and α-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid/kainic acid (AMPA/KA-GluR1, GluR2, GluR2/3, GluR4, and GluR5/6/7) in the inferior olive of postnatal rats up to adulthood. Immunoreactivity for distinct receptor subunits was predominantly localized in the soma and dendrites of neurons. Semi-quantification showed that the overall immunoreactivity in the inferior olive of adults was intense for GluR1, moderate for NR1 and NR2A/B, and low for GluR2, GluR2/3, GluR4, and GluR5/6/7. At P7, GluR1 was restricted to the dorsomedial cell column, subnucleus beta, principal nucleus and ventrolateral protrusion while the other subunits were found in all subnuclei of the inferior olive. The immunoreactivities for all glutamate receptor subunits ranged from low to moderate. As the rats matured, the immunoreactivity of GluR4 decreased after the second postnatal week, while those of the other subunits showed a general trend of increase, reaching adult level during the third postnatal week. Double immunofluorescence revealed that all NR1-containing neurons exhibited NR2A/B immunoreactivity, indicating that native NMDA receptors comprise of hetero-oligomeric combinations of NR1 and NR2A/B. Furthermore, co-localization of NMDA and AMPA/KA receptor subunits was demonstrated in individual neurons of the inferior olive. All NR1-containing neurons exhibited GluR1 immunoreactivity, and all NR2A/B-containing neurons showed GluR5/6/7 immunoreactivity. Our data suggest that NMDA and AMPA/KA receptors are involved in glutamate-mediated neurotransmission, contributing to synaptic plasticity and reorganization of circuitry in the inferior olive during postnatal development. © 2005 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainresen_HK
dc.relation.ispartofBrain Researchen_HK
dc.rightsBrain Research. Copyright © Elsevier BV.en_HK
dc.subjectAMPA/KA receptoren_HK
dc.subjectCo-localizationen_HK
dc.subjectImmunohistochemistryen_HK
dc.subjectInferior oliveen_HK
dc.subjectNMDA receptoren_HK
dc.subject.meshAgingen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshOlivary Nucleus - growth & development - physiologyen_HK
dc.subject.meshProtein Subunits - metabolismen_HK
dc.subject.meshRatsen_HK
dc.subject.meshReceptors, AMPA - metabolismen_HK
dc.subject.meshReceptors, Kainic Acid - metabolismen_HK
dc.subject.meshReceptors, N-Methyl-D-Aspartate - metabolismen_HK
dc.titleDifferential expression of NMDA and AMPA/KA receptor subunits in the inferior olive of postnatal ratsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-8993&volume=1067&issue=1&spage=103&epage=114&date=2006&atitle=Differential+expression+of+NMDA+and+AMPA/KA+receptor+subunits+in+the+inferior+olive+of+postnatal+ratsen_HK
dc.identifier.emailLai, CH: chlaib@hku.hken_HK
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hken_HK
dc.identifier.emailChan, YS: yschan@hkucc.hku.hken_HK
dc.identifier.authorityLai, CH=rp00396en_HK
dc.identifier.authorityShum, DKY=rp00321en_HK
dc.identifier.authorityChan, YS=rp00318en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.brainres.2005.10.054en_HK
dc.identifier.pmid16376317-
dc.identifier.scopuseid_2-s2.0-31144455841en_HK
dc.identifier.hkuros121193en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-31144455841&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1067en_HK
dc.identifier.issue1en_HK
dc.identifier.spage103en_HK
dc.identifier.epage114en_HK
dc.identifier.isiWOS:000235356500013-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridChen, LW=7409444941en_HK
dc.identifier.scopusauthoridTse, YC=7005116869en_HK
dc.identifier.scopusauthoridLi, C=8055499400en_HK
dc.identifier.scopusauthoridGuan, ZL=7202542208en_HK
dc.identifier.scopusauthoridLai, CH=7403086597en_HK
dc.identifier.scopusauthoridYung, KKL=13605496000en_HK
dc.identifier.scopusauthoridShum, DKY=7004824447en_HK
dc.identifier.scopusauthoridChan, YS=7403676627en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats