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Article: In vitro chondrogenic differentiation of human mesenchymal stem cells in collagen microspheres: Influence of cell seeding density and collagen concentration

TitleIn vitro chondrogenic differentiation of human mesenchymal stem cells in collagen microspheres: Influence of cell seeding density and collagen concentration
Authors
KeywordsCartilage
Chondrogenic differentiation
Collagen
Mesenchymal stem cell
Microspheres
Tissue engineering
Issue Date2008
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biomaterials
Citation
Biomaterials, 2008, v. 29 n. 22, p. 3201-3212 How to Cite?
AbstractGiven the inadequacies of existing repair strategies for cartilage injuries, tissue engineering approach using biomaterials and stem cells offers new hope for better treatments. Recently, we have fabricated injectable collagen-human mesenchymal stem cell (hMSC) microspheres using microencapsulation. Apart from providing a protective matrix for cell delivery, the collagen microspheres may also act as a bio-mimetic matrix facilitating the functional remodeling of hMSCs. In this study, whether the encapsulated hMSCs can be pre-differentiated into chondrogenic phenotype prior to implantation has been investigated. The effects of cell seeding density and collagen concentration on the chondrogenic differentiation potential of hMSCs have been studied. An in vivo implantation study has also been conducted. Fabrication of cartilage-like tissue micro-masses was demonstrated by positive immunohistochemical staining for cartilage-specific extracellular matrix components including type II collagen and aggrecan. The meshwork of collagen fibers was remodeled into a highly ordered microstructure, characterized by thick and parallel bundles, upon differentiation. Higher cell seeding density and higher collagen concentration favored the chondrogenic differentiation of hMSCs, yielding increased matrix production and mechanical strength of the micro-masses. These micro-masses were also demonstrated to integrate well with the host tissue in NOD/SCID mice. © 2008 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/68322
ISSN
2015 Impact Factor: 8.387
2015 SCImago Journal Rankings: 3.565
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHui, TYen_HK
dc.contributor.authorCheung, KMCen_HK
dc.contributor.authorCheung, WLen_HK
dc.contributor.authorChan, Den_HK
dc.contributor.authorChan, BPen_HK
dc.date.accessioned2010-09-06T06:03:29Z-
dc.date.available2010-09-06T06:03:29Z-
dc.date.issued2008en_HK
dc.identifier.citationBiomaterials, 2008, v. 29 n. 22, p. 3201-3212en_HK
dc.identifier.issn0142-9612en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68322-
dc.description.abstractGiven the inadequacies of existing repair strategies for cartilage injuries, tissue engineering approach using biomaterials and stem cells offers new hope for better treatments. Recently, we have fabricated injectable collagen-human mesenchymal stem cell (hMSC) microspheres using microencapsulation. Apart from providing a protective matrix for cell delivery, the collagen microspheres may also act as a bio-mimetic matrix facilitating the functional remodeling of hMSCs. In this study, whether the encapsulated hMSCs can be pre-differentiated into chondrogenic phenotype prior to implantation has been investigated. The effects of cell seeding density and collagen concentration on the chondrogenic differentiation potential of hMSCs have been studied. An in vivo implantation study has also been conducted. Fabrication of cartilage-like tissue micro-masses was demonstrated by positive immunohistochemical staining for cartilage-specific extracellular matrix components including type II collagen and aggrecan. The meshwork of collagen fibers was remodeled into a highly ordered microstructure, characterized by thick and parallel bundles, upon differentiation. Higher cell seeding density and higher collagen concentration favored the chondrogenic differentiation of hMSCs, yielding increased matrix production and mechanical strength of the micro-masses. These micro-masses were also demonstrated to integrate well with the host tissue in NOD/SCID mice. © 2008 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biomaterialsen_HK
dc.relation.ispartofBiomaterialsen_HK
dc.rightsBiomaterials. Copyright © Elsevier BV.en_HK
dc.subjectCartilageen_HK
dc.subjectChondrogenic differentiationen_HK
dc.subjectCollagenen_HK
dc.subjectMesenchymal stem cellen_HK
dc.subjectMicrospheresen_HK
dc.subjectTissue engineeringen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCell Counten_HK
dc.subject.meshCell Differentiationen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshChondrocytes - cytology - metabolismen_HK
dc.subject.meshCollagen - metabolismen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshMesenchymal Stem Cell Transplantationen_HK
dc.subject.meshMesenchymal Stem Cells - cytology - metabolismen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Inbred NODen_HK
dc.subject.meshMice, SCIDen_HK
dc.subject.meshMicrospheresen_HK
dc.subject.meshTissue Engineering - methodsen_HK
dc.titleIn vitro chondrogenic differentiation of human mesenchymal stem cells in collagen microspheres: Influence of cell seeding density and collagen concentrationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0142-9612&volume=29&spage=3201&epage=3212&date=2008&atitle=In+vitro+chondrogenic+differentiation+of+human+mesenchymal+stem+cells+in+collagen+microspheres:+influence+of+cell+seeding+density+and+collagen+concentrationen_HK
dc.identifier.emailCheung, KMC:cheungmc@hku.hken_HK
dc.identifier.emailCheung, WL:wlcheung@hkucc.hku.hken_HK
dc.identifier.emailChan, D:chand@hkucc.hku.hken_HK
dc.identifier.emailChan, BP:bpchan@hkucc.hku.hken_HK
dc.identifier.authorityCheung, KMC=rp00387en_HK
dc.identifier.authorityCheung, WL=rp00103en_HK
dc.identifier.authorityChan, D=rp00540en_HK
dc.identifier.authorityChan, BP=rp00087en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.biomaterials.2008.04.001en_HK
dc.identifier.pmid18462789-
dc.identifier.scopuseid_2-s2.0-43549099478en_HK
dc.identifier.hkuros145972en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-43549099478&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume29en_HK
dc.identifier.issue22en_HK
dc.identifier.spage3201en_HK
dc.identifier.epage3212en_HK
dc.identifier.eissn1878-5905-
dc.identifier.isiWOS:000257012700005-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridHui, TY=36192803300en_HK
dc.identifier.scopusauthoridCheung, KMC=7402406754en_HK
dc.identifier.scopusauthoridCheung, WL=7202743084en_HK
dc.identifier.scopusauthoridChan, D=7402216545en_HK
dc.identifier.scopusauthoridChan, BP=7201530390en_HK

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