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Article: Calcium oxalate crystallizing properties of polyanions elaborated by cultured renal proximal tubular cells

TitleCalcium oxalate crystallizing properties of polyanions elaborated by cultured renal proximal tubular cells
Authors
Issue Date1995
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00240/index.htm
Citation
Urological Research, 1995, v. 23 n. 2, p. 103-110 How to Cite?
AbstractTo study the influence of renal polyanions on crystallization of urinary calcium oxalate, we recovered polyanionic macromolecules from media conditioned by primary cultures of renal proximal tubular epithelial cells of rats in serum-free, hormonally defined medium. Cells cultured on microporous supports showed a higher degree of morphological and functional proximal differentiation into a polarized monolayer than those on plastic impervious substrata. Papainization of the polyanions yielded the glycosaminoglycans chondroitin/dermatan sulphate and heparan sulphate. These accounted respectively for 60% and 80% of the crystal nucleation-promoting activities of polyanions recovered from the apical and basal media conditioned by polarized cultures on microporous supports. Similar relative activities were observed among the urinary glycosaminoglycans and polyanions similarly tested. Primary cultures of polarized proximal tubular epithelial cells are useful then as an in vitro model to study the crystallizing activities of polyanionic macromolecules produced by renal cells.
Persistent Identifierhttp://hdl.handle.net/10722/68321
ISSN
2014 Impact Factor: 1.394
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorShum, DKYen_HK
dc.contributor.authorLiong, Een_HK
dc.date.accessioned2010-09-06T06:03:28Z-
dc.date.available2010-09-06T06:03:28Z-
dc.date.issued1995en_HK
dc.identifier.citationUrological Research, 1995, v. 23 n. 2, p. 103-110en_HK
dc.identifier.issn0300-5623en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68321-
dc.description.abstractTo study the influence of renal polyanions on crystallization of urinary calcium oxalate, we recovered polyanionic macromolecules from media conditioned by primary cultures of renal proximal tubular epithelial cells of rats in serum-free, hormonally defined medium. Cells cultured on microporous supports showed a higher degree of morphological and functional proximal differentiation into a polarized monolayer than those on plastic impervious substrata. Papainization of the polyanions yielded the glycosaminoglycans chondroitin/dermatan sulphate and heparan sulphate. These accounted respectively for 60% and 80% of the crystal nucleation-promoting activities of polyanions recovered from the apical and basal media conditioned by polarized cultures on microporous supports. Similar relative activities were observed among the urinary glycosaminoglycans and polyanions similarly tested. Primary cultures of polarized proximal tubular epithelial cells are useful then as an in vitro model to study the crystallizing activities of polyanionic macromolecules produced by renal cells.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00240/index.htmen_HK
dc.relation.ispartofUrological Researchen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCalcium Oxalate - metabolismen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshCrystallizationen_HK
dc.subject.meshCulture Media, Conditioned - metabolismen_HK
dc.subject.meshGlycosaminoglycans - metabolismen_HK
dc.subject.meshKidney Tubules, Proximal - cytology - metabolismen_HK
dc.subject.meshPolyamines - metabolismen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.subject.meshUrine - chemistryen_HK
dc.titleCalcium oxalate crystallizing properties of polyanions elaborated by cultured renal proximal tubular cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0300-5623&volume=23&spage=103&epage=110&date=1995&atitle=Calcium+oxalate+crystallizing+properties+of+polyanions+elaborated+by+cultured+renal+proximal+tubular+cellsen_HK
dc.identifier.emailShum, DKY:shumdkhk@hkucc.hku.hken_HK
dc.identifier.authorityShum, DKY=rp00321en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/BF00307940en_HK
dc.identifier.pmid7676532en_HK
dc.identifier.scopuseid_2-s2.0-0029016911en_HK
dc.identifier.hkuros1901en_HK
dc.identifier.volume23en_HK
dc.identifier.issue2en_HK
dc.identifier.spage103en_HK
dc.identifier.epage110en_HK
dc.identifier.isiWOS:A1995RC35300005-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridShum, DKY=7004824447en_HK
dc.identifier.scopusauthoridLiong, E=35938810100en_HK

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