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Article: Quantitative electrophoretic study of the modification of sperm plasma membrane by the ampullary gland in the golden hamster

TitleQuantitative electrophoretic study of the modification of sperm plasma membrane by the ampullary gland in the golden hamster
Authors
Issue Date1995
PublisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/01485016.asp
Citation
Archives Of Andrology, 1995, v. 34 n. 2, p. 53-61 How to Cite?
AbstractPlasma membrane proteins were extracted either from epididymal sperm after incubation with ampullary gland secretion or from uterine sperm derived from surgically treated males belonging to the following groups: TX, excision of all accessory sex glands (ASG); AGX, bilateral excision of ampullary glands; AG, excision of all ASG except ampullary glands; and SH, sham-operated. Total membrane protein, glycoprotein, and SDS-PAGE of individual polypeptide subunits were quantified. After incubation with ampullary gland secretion, both protein and glycoprotein concentrations of epididymal sperm membrane were increased. The protein profile was also significantly altered, with the removal of the 43- and 71-kD subunits and the addition of the 36- and 50-kD subunits. The in vitro results confirmed this proteolytic effect of ampullary gland and other ASG on the 43- and 71-kD subunits, despite a reduction in membrane protein concentration. Modification of the 17-, 20-, 25-, 28-, 56-, and 66-kD proteins were also observed. This report is the first demonstration that the ampullary gland is capable of modifying proteins on the sperm surface.
Persistent Identifierhttp://hdl.handle.net/10722/68316
ISSN
2009 Impact Factor: 0.891
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChow, PHen_HK
dc.contributor.authorYuen, ACYen_HK
dc.contributor.authorCheng, LYLen_HK
dc.date.accessioned2010-09-06T06:03:26Z-
dc.date.available2010-09-06T06:03:26Z-
dc.date.issued1995en_HK
dc.identifier.citationArchives Of Andrology, 1995, v. 34 n. 2, p. 53-61en_HK
dc.identifier.issn0148-5016en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68316-
dc.description.abstractPlasma membrane proteins were extracted either from epididymal sperm after incubation with ampullary gland secretion or from uterine sperm derived from surgically treated males belonging to the following groups: TX, excision of all accessory sex glands (ASG); AGX, bilateral excision of ampullary glands; AG, excision of all ASG except ampullary glands; and SH, sham-operated. Total membrane protein, glycoprotein, and SDS-PAGE of individual polypeptide subunits were quantified. After incubation with ampullary gland secretion, both protein and glycoprotein concentrations of epididymal sperm membrane were increased. The protein profile was also significantly altered, with the removal of the 43- and 71-kD subunits and the addition of the 36- and 50-kD subunits. The in vitro results confirmed this proteolytic effect of ampullary gland and other ASG on the 43- and 71-kD subunits, despite a reduction in membrane protein concentration. Modification of the 17-, 20-, 25-, 28-, 56-, and 66-kD proteins were also observed. This report is the first demonstration that the ampullary gland is capable of modifying proteins on the sperm surface.en_HK
dc.languageengen_HK
dc.publisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/01485016.aspen_HK
dc.relation.ispartofArchives of Andrologyen_HK
dc.rightsArchives of Andrology. Copyright © Informa Healthcare.en_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCricetinaeen_HK
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_HK
dc.subject.meshEpididymis - metabolismen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMembrane Proteins - metabolismen_HK
dc.subject.meshMesocricetusen_HK
dc.subject.meshSpermatozoa - metabolismen_HK
dc.subject.meshVas Deferens - metabolism - secretionen_HK
dc.titleQuantitative electrophoretic study of the modification of sperm plasma membrane by the ampullary gland in the golden hamsteren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0148-5016&volume=34&spage=53&epage=61&date=1995&atitle=Quantitative+electrophoretic+study+of+the+modification+of+sperm+plasma+membrane+by+the+ampullary+gland+in+the+golden+hamsteren_HK
dc.identifier.emailCheng, LYL:lcheng@hkucc.hku.hken_HK
dc.identifier.authorityCheng, LYL=rp00317en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid7786088en_HK
dc.identifier.scopuseid_2-s2.0-0028918174en_HK
dc.identifier.hkuros1795en_HK
dc.identifier.volume34en_HK
dc.identifier.issue2en_HK
dc.identifier.spage53en_HK
dc.identifier.epage61en_HK
dc.identifier.isiWOS:A1995QR48900001-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridChow, PH=7202656919en_HK
dc.identifier.scopusauthoridYuen, ACY=7006290143en_HK
dc.identifier.scopusauthoridCheng, LYL=7403337122en_HK

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