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Article: Kinetics and synergistic effects of siRNAs targeting structural and replicase genes of SARS-associated coronavirus

TitleKinetics and synergistic effects of siRNAs targeting structural and replicase genes of SARS-associated coronavirus
Authors
He, MLZheng, BjChen, YWong, KLHuang, JDLin, MCPeng, YYuen, KYSung, JJYKung, Hf
KeywordsInhibition of SCoV reproduction
RNA interference
SARS-associated coronavirus
siRNA
Viral kinetics
Issue Date2006
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/febslet
Citation
Febs Letters, 2006, v. 580 n. 10, p. 2414-2420 How to Cite?
DOI: http://dx.doi.org/10.1016/j.febslet.2006.03.066
AbstractSARS-associated coronavirus was identified as the etiological agent of severe acute respiratory syndrome and a large virus pool was identified in wild animals. Virus generates drug resistance through fast mutagenesis and escapes antiviral treatment. siRNAs targeting different genes would be an alternative for overcoming drug resistance. Here, we report effective siRNAs targeting structural genes (i.e., spike, envelope, membrane, and nucleocapsid) and their antiviral kinetics. We also showed the synergistic effects of two siRNAs targeting different functional genes at a very low dose. Our findings may pave a way to develop cost effective siRNA agents for antiviral therapy in the future. © 2006.
Persistent Identifierhttp://hdl.handle.net/10722/68315
ISSN
0014-5793
2023 Impact Factor: 3.0
2023 SCImago Journal Rankings: 1.208
ISI Accession Number ID
WOS:000237433500004
References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorHe, MLen_HK
dc.contributor.authorZheng, Bjen_HK
dc.contributor.authorChen, Yen_HK
dc.contributor.authorWong, KLen_HK
dc.contributor.authorHuang, JDen_HK
dc.contributor.authorLin, MCen_HK
dc.contributor.authorPeng, Yen_HK
dc.contributor.authorYuen, KYen_HK
dc.contributor.authorSung, JJYen_HK
dc.contributor.authorKung, Hfen_HK
dc.date.accessioned2010-09-06T06:03:25Z-
dc.date.available2010-09-06T06:03:25Z-
dc.date.issued2006en_HK
dc.identifier.citationFebs Letters, 2006, v. 580 n. 10, p. 2414-2420en_HK
dc.identifier.issn0014-5793en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68315-
dc.description.abstractSARS-associated coronavirus was identified as the etiological agent of severe acute respiratory syndrome and a large virus pool was identified in wild animals. Virus generates drug resistance through fast mutagenesis and escapes antiviral treatment. siRNAs targeting different genes would be an alternative for overcoming drug resistance. Here, we report effective siRNAs targeting structural genes (i.e., spike, envelope, membrane, and nucleocapsid) and their antiviral kinetics. We also showed the synergistic effects of two siRNAs targeting different functional genes at a very low dose. Our findings may pave a way to develop cost effective siRNA agents for antiviral therapy in the future. © 2006.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/febsleten_HK
dc.relation.ispartofFEBS Lettersen_HK
dc.rightsF E B S Letters. Copyright © Elsevier BV.en_HK
dc.subjectInhibition of SCoV reproductionen_HK
dc.subjectRNA interferenceen_HK
dc.subjectSARS-associated coronavirusen_HK
dc.subjectsiRNAen_HK
dc.subjectViral kineticsen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshCytopathogenic Effect, Viralen_HK
dc.subject.meshDNA Primersen_HK
dc.subject.meshKineticsen_HK
dc.subject.meshMacaca mulattaen_HK
dc.subject.meshRNA, Small Interferingen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshSARS Virus - enzymology - genetics - pathogenicity - physiologyen_HK
dc.subject.meshViral Proteins - geneticsen_HK
dc.subject.meshVirus Replicationen_HK
dc.titleKinetics and synergistic effects of siRNAs targeting structural and replicase genes of SARS-associated coronavirusen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0014-5793&volume=580&issue=10&spage=2414&epage=2420&date=2006&atitle=Kinetics+and+synergistic+effects+of+siRNAs+targeting+structural+and+replicase+genes+of+SARS-associated+coronavirus.en_HK
dc.identifier.emailZheng, Bj:bzheng@hkucc.hku.hken_HK
dc.identifier.emailHuang, JD:jdhuang@hkucc.hku.hken_HK
dc.identifier.emailLin, MC:mcllin@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityZheng, Bj=rp00353en_HK
dc.identifier.authorityHuang, JD=rp00451en_HK
dc.identifier.authorityLin, MC=rp00746en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.febslet.2006.03.066en_HK
dc.identifier.pmid16638566-
dc.identifier.scopuseid_2-s2.0-33646092274en_HK
dc.identifier.hkuros115504en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33646092274&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume580en_HK
dc.identifier.issue10en_HK
dc.identifier.spage2414en_HK
dc.identifier.epage2420en_HK
dc.identifier.isiWOS:000237433500004-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridHe, ML=35080389700en_HK
dc.identifier.scopusauthoridZheng, Bj=7201780588en_HK
dc.identifier.scopusauthoridChen, Y=7601425634en_HK
dc.identifier.scopusauthoridWong, KL=7404758889en_HK
dc.identifier.scopusauthoridHuang, JD=8108660600en_HK
dc.identifier.scopusauthoridLin, MC=7404816359en_HK
dc.identifier.scopusauthoridPeng, Y=7403419265en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK
dc.identifier.scopusauthoridSung, JJY=35405352400en_HK
dc.identifier.scopusauthoridKung, Hf=7402514190en_HK
dc.identifier.issnl0014-5793-

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