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Article: Cold-inducible RNA binding protein is required for the expression of adhesion molecules and embryonic cell movement in Xenopus laevis

TitleCold-inducible RNA binding protein is required for the expression of adhesion molecules and embryonic cell movement in Xenopus laevis
Authors
KeywordsCell movement
Cold-inducible RNA binding protein
Morphogenetic lineage migration
Neural development
Xenopus
Issue Date2006
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2006, v. 344 n. 1, p. 416-424 How to Cite?
AbstractWe have previously shown that the Xenopus homologue of cold-inducible RNA binding protein, XCIRP-1, is required for the morphogenetic migration of the pronephros during embryonic development. However, the underlying molecular mechanisms remain elusive. Here, we report that XCIRP is essential for embryonic cell movement, as suppression of XCIRP by microinjection of anti-sense mRNA and morpholino antisense oligonucleotides (MOs) significantly reduced protein expression, inhibited the cell migration rate, and inhibited eFGF and activin-induced animal cap elongation. By immunoprecipitation and RT-PCR, we further showed that the mRNA of a panel of adhesion molecules, including αE- and β-catenin, C- and E-cadherin, and paraxial proto-cadherin, are the targets of XCIRP. Consistently, in animal cap explant studies, suppression of XCIRP by MOs inhibited the expression of these adhesion molecules, while over-expression of sense XCIRP-1 mRNA fully rescued this inhibition. Taken together, these results suggest for the first time that XCIRP is required to maintain the expression of adhesion molecules and cell movement during embryonic development. © 2006 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/68313
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPeng, Yen_HK
dc.contributor.authorYang, PHen_HK
dc.contributor.authorTanner, JAen_HK
dc.contributor.authorHuang, JDen_HK
dc.contributor.authorLi, Men_HK
dc.contributor.authorLee, HFen_HK
dc.contributor.authorXu, RHen_HK
dc.contributor.authorKung, HFen_HK
dc.contributor.authorLin, MCMen_HK
dc.date.accessioned2010-09-06T06:03:24Z-
dc.date.available2010-09-06T06:03:24Z-
dc.date.issued2006en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 2006, v. 344 n. 1, p. 416-424en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/68313-
dc.description.abstractWe have previously shown that the Xenopus homologue of cold-inducible RNA binding protein, XCIRP-1, is required for the morphogenetic migration of the pronephros during embryonic development. However, the underlying molecular mechanisms remain elusive. Here, we report that XCIRP is essential for embryonic cell movement, as suppression of XCIRP by microinjection of anti-sense mRNA and morpholino antisense oligonucleotides (MOs) significantly reduced protein expression, inhibited the cell migration rate, and inhibited eFGF and activin-induced animal cap elongation. By immunoprecipitation and RT-PCR, we further showed that the mRNA of a panel of adhesion molecules, including αE- and β-catenin, C- and E-cadherin, and paraxial proto-cadherin, are the targets of XCIRP. Consistently, in animal cap explant studies, suppression of XCIRP by MOs inhibited the expression of these adhesion molecules, while over-expression of sense XCIRP-1 mRNA fully rescued this inhibition. Taken together, these results suggest for the first time that XCIRP is required to maintain the expression of adhesion molecules and cell movement during embryonic development. © 2006 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.subjectCell movementen_HK
dc.subjectCold-inducible RNA binding proteinen_HK
dc.subjectMorphogenetic lineage migrationen_HK
dc.subjectNeural developmenten_HK
dc.subjectXenopusen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCell Adhesion Molecules - genetics - metabolismen_HK
dc.subject.meshCell Lineage - geneticsen_HK
dc.subject.meshCell Movement - geneticsen_HK
dc.subject.meshEmbryo, Nonmammalian - cytologyen_HK
dc.subject.meshGene Expression Regulation, Developmentalen_HK
dc.subject.meshNerve Tissue Proteins - antagonists & inhibitors - genetics - physiologyen_HK
dc.subject.meshRNA, Antisense - genetics - pharmacologyen_HK
dc.subject.meshRNA-Binding Proteins - antagonists & inhibitors - genetics - physiologyen_HK
dc.subject.meshXenopus Proteins - antagonists & inhibitors - genetics - physiologyen_HK
dc.subject.meshXenopus laevis - embryology - genetics - metabolismen_HK
dc.titleCold-inducible RNA binding protein is required for the expression of adhesion molecules and embryonic cell movement in Xenopus laevisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-291X&volume=344&spage=416&epage=424&date=2006&atitle=Cold-inducible+RNA+binding+protein+is+required+for+the+expression+of+adhesion+molecules+and+embryonic+cell+movement+in+Xenopus+laevisen_HK
dc.identifier.emailTanner, JA:jatanner@hku.hken_HK
dc.identifier.emailHuang, JD:jdhuang@hkucc.hku.hken_HK
dc.identifier.emailLin, MCM:mcllin@hkucc.hku.hken_HK
dc.identifier.authorityTanner, JA=rp00495en_HK
dc.identifier.authorityHuang, JD=rp00451en_HK
dc.identifier.authorityLin, MCM=rp00746en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.bbrc.2006.03.086en_HK
dc.identifier.pmid16600183-
dc.identifier.scopuseid_2-s2.0-33646026703en_HK
dc.identifier.hkuros115423en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33646026703&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume344en_HK
dc.identifier.issue1en_HK
dc.identifier.spage416en_HK
dc.identifier.epage424en_HK
dc.identifier.isiWOS:000237408000059-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridPeng, Y=7403419265en_HK
dc.identifier.scopusauthoridYang, PH=24340289000en_HK
dc.identifier.scopusauthoridTanner, JA=35513993000en_HK
dc.identifier.scopusauthoridHuang, JD=8108660600en_HK
dc.identifier.scopusauthoridLi, M=36067425800en_HK
dc.identifier.scopusauthoridLee, HF=13007272100en_HK
dc.identifier.scopusauthoridXu, RH=35243812400en_HK
dc.identifier.scopusauthoridKung, HF=7402514190en_HK
dc.identifier.scopusauthoridLin, MCM=7404816359en_HK

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