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Article: Hoxb3 vagal neural crest-specific enhancer element for controlling enteric nervous system development

TitleHoxb3 vagal neural crest-specific enhancer element for controlling enteric nervous system development
Authors
KeywordsEnhancer element
Enteric nervous system
Gut
Hoxb3
Intestinal aganglionosis
Transgenic mice
Vagal neural crest
Issue Date2005
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/38417
Citation
Developmental Dynamics, 2005, v. 233 n. 2, p. 473-483 How to Cite?
AbstractThe neural and glial cells of the intrinsic ganglia of the enteric nervous system (ENS) are derived from the hindbrain neural crest at the vagal level. The Hoxb3 gene is expressed in the vagal neural crest and in the enteric ganglia of the developing gut during embryogenesis. We have identified a cis-acting enhancer element b3IIIa in the Hoxb3 gene locus. In this study, by transgenic mice analysis, we examined the tissue specificity of the b3IIIa enhancer element using the lacZ reporter gene, with emphasis on the vagal neural crest cells and their derivatives in the developing gut. We found that the b3IIIa-lacZ transgene marks only the vagal region and not the trunk or sacral region. Using cellular markers, we showed that the b3IIIa-lacZ transgene was expressed in a subset of enteric neuroblasts during early development of the gut, and the expression was maintained in differentiated neurons of the myenteric plexus at later stages. The specificity of the b3IIIa enhancer in directing gene expression in the developing ENS was further supported by genetic analysis using the Dom mutant, a spontaneous mouse model of Hirschsprung's disease characterized by the absence of enteric ganglia in the distal gut. The colonization of lacZ-expressing cells in the large intestine was incomplete in all the Dom/b3IIIa-lacZ hybrid mutants we examined. To our knowledge, this is the only vagal neural crest-specific genetic regulatory element identified to date. This element could be used for a variety of genetic manipulations and in establishing transgenic mouse models for studying the development of the ENS. © 2005 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/68312
ISSN
2015 Impact Factor: 2.198
2015 SCImago Journal Rankings: 1.726
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, KKen_HK
dc.contributor.authorChen, YSen_HK
dc.contributor.authorYau, TOen_HK
dc.contributor.authorFu, Men_HK
dc.contributor.authorLui, VCHen_HK
dc.contributor.authorTam, PKHen_HK
dc.contributor.authorSham, MHen_HK
dc.date.accessioned2010-09-06T06:03:23Z-
dc.date.available2010-09-06T06:03:23Z-
dc.date.issued2005en_HK
dc.identifier.citationDevelopmental Dynamics, 2005, v. 233 n. 2, p. 473-483en_HK
dc.identifier.issn1058-8388en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68312-
dc.description.abstractThe neural and glial cells of the intrinsic ganglia of the enteric nervous system (ENS) are derived from the hindbrain neural crest at the vagal level. The Hoxb3 gene is expressed in the vagal neural crest and in the enteric ganglia of the developing gut during embryogenesis. We have identified a cis-acting enhancer element b3IIIa in the Hoxb3 gene locus. In this study, by transgenic mice analysis, we examined the tissue specificity of the b3IIIa enhancer element using the lacZ reporter gene, with emphasis on the vagal neural crest cells and their derivatives in the developing gut. We found that the b3IIIa-lacZ transgene marks only the vagal region and not the trunk or sacral region. Using cellular markers, we showed that the b3IIIa-lacZ transgene was expressed in a subset of enteric neuroblasts during early development of the gut, and the expression was maintained in differentiated neurons of the myenteric plexus at later stages. The specificity of the b3IIIa enhancer in directing gene expression in the developing ENS was further supported by genetic analysis using the Dom mutant, a spontaneous mouse model of Hirschsprung's disease characterized by the absence of enteric ganglia in the distal gut. The colonization of lacZ-expressing cells in the large intestine was incomplete in all the Dom/b3IIIa-lacZ hybrid mutants we examined. To our knowledge, this is the only vagal neural crest-specific genetic regulatory element identified to date. This element could be used for a variety of genetic manipulations and in establishing transgenic mouse models for studying the development of the ENS. © 2005 Wiley-Liss, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/38417en_HK
dc.relation.ispartofDevelopmental Dynamicsen_HK
dc.rightsDevelopmental Dynamics. Copyright © John Wiley & Sons, Inc.en_HK
dc.subjectEnhancer elementen_HK
dc.subjectEnteric nervous systemen_HK
dc.subjectGuten_HK
dc.subjectHoxb3en_HK
dc.subjectIntestinal aganglionosisen_HK
dc.subjectTransgenic miceen_HK
dc.subjectVagal neural cresten_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshEnhancer Elements, Genetic - geneticsen_HK
dc.subject.meshGene Expression Regulation, Developmental - geneticsen_HK
dc.subject.meshHomeodomain Proteins - geneticsen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshLac Operon - geneticsen_HK
dc.subject.meshMegacolon - embryology - genetics - metabolismen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Transgenicen_HK
dc.subject.meshNervous System - embryology - metabolismen_HK
dc.subject.meshNeural Crest - embryology - metabolismen_HK
dc.subject.meshOrgan Specificityen_HK
dc.titleHoxb3 vagal neural crest-specific enhancer element for controlling enteric nervous system developmenten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1058-8388&volume=233&spage=473&epage=483&date=2005&atitle=Hoxb3+vagal+neural+crest-specific+enhancer+element+for+controlling+enteric+nervous+system+developmenten_HK
dc.identifier.emailLui, VCH: vchlui@hkucc.hku.hken_HK
dc.identifier.emailTam, PKH: paultam@hkucc.hku.hken_HK
dc.identifier.emailSham, MH: mhsham@hkucc.hku.hken_HK
dc.identifier.authorityLui, VCH=rp00363en_HK
dc.identifier.authorityTam, PKH=rp00060en_HK
dc.identifier.authoritySham, MH=rp00380en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/dvdy.20347en_HK
dc.identifier.pmid15768390-
dc.identifier.scopuseid_2-s2.0-19544378341en_HK
dc.identifier.hkuros98054en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-19544378341&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume233en_HK
dc.identifier.issue2en_HK
dc.identifier.spage473en_HK
dc.identifier.epage483en_HK
dc.identifier.isiWOS:000229353100023-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChan, KK=7406034649en_HK
dc.identifier.scopusauthoridChen, YS=34975255700en_HK
dc.identifier.scopusauthoridYau, TO=7006540669en_HK
dc.identifier.scopusauthoridFu, M=49761323800en_HK
dc.identifier.scopusauthoridLui, VCH=7004231344en_HK
dc.identifier.scopusauthoridTam, PKH=7202539421en_HK
dc.identifier.scopusauthoridSham, MH=7003729109en_HK

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