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Article: Impact of mutations of cartilage matrix genes on matrix structure, gene activity and chondrogenesis

TitleImpact of mutations of cartilage matrix genes on matrix structure, gene activity and chondrogenesis
Authors
Issue Date2001
PublisherWB Saunders Co Ltd. The Journal's web site is located at http://www.elsevier.com/locate/joca
Citation
Osteoarthritis And Cartilage, 2001, v. 9 SUPPL. A, p. S160-S173 How to Cite?
AbstractObjective: Chondrocytes in the growth plate at different stages of differentiation synthesize characteristic extracellular matrix (ECM) components. Mutations in some ECM genes result in chondrodysplasia in humans and mice. We aimed to evaluate the impact of loss- and gain-of-function mutations of ECM genes on matrix structure, gene expression and formation of the growth plate. Design: We review information on the impact of deficiencies in proteoglycans, and types X and II collagens on skeletal development. Additionally, we compare the impact of a glycine904 to cysteine (G904C) mutation in the triple helical coding domain of mouse Col2a1 with two previously reported Col2a1 mutations (exon7 deletion (Del1) and G85C). The G904C Col2a1 gene was introduced as a transgene into mice. Transgenic newborn mice were examined for skeletal development. The histology of the epiphyseal cartilage and the growth plate, and the ultrastructure of chondrocytes and collagen fibrillar morphology in the ECM were studied in 18.5-day transgenic and wild-type fetuses. The distribution of the mRNAs for Col2a1, Col11a1, Col9a1, Matn1, Agc and Ihh in the growth plate of 18.5-day G904C/G904C and wild type fetuses were compared by in situ hybridization. Results: Heterozygous transgenic mice harbouring five copies of the G904C Col2a1 transgene developed skeletal abnormalities and dwarfism. Homozygous G904C/G904C mice died at birth, showing cleft palate, disrupted zonation of chondrocytes and reduction of the zone of hypertrophic chondrocytes. Fewer collagen fibrils were found in ECM of the cartilage. Rough endoplasmic reticulum of the chondrocytes of G904C/ + and G904C/G904C mice was distended. In G904C/G904C mutant mice, Agc gene activity was extended to the hypertrophic zone. Expression of the other genes studied was unchanged. Calcified materials that were not found normally in the maturing and only at low abundance in the hypertrophic zones of the wild type growth plate, were present in these zones in G904C/G904C mice. Despite phenotypic similarities for the G904C and Del1 mice, reduced expression of types I, II, IX, X collagens and aggrecan were reported for the latter mutation. Changes in gene activity and matrix organization in the growth plate also accompanied deficiencies in aggrecan, perlecan and collagen II. Conclusions: The data suggest that a single amino acid alteration in collagen II could lead to skeletal abnormalities through multiple secondary effects on the synthesis and assembly of ECM components. The functional impact of mutations of ECM genes reveals that chondrodysplasia is caused not just by the formation of abnormal matrix molecules, but that the alteration of one ECM component may lead to a cascade of disruption of other gene activities in chondrocytes which collectively contribute to the pathological changes in the architecture of the growth plate. © 2001 OsteoArthritis Research Society International.
Persistent Identifierhttp://hdl.handle.net/10722/68301
ISSN
2015 Impact Factor: 4.535
2015 SCImago Journal Rankings: 1.901
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSo, CLen_HK
dc.contributor.authorKaluarachchi, Ken_HK
dc.contributor.authorTam, PPLen_HK
dc.contributor.authorCheah, KSEen_HK
dc.date.accessioned2010-09-06T06:03:17Z-
dc.date.available2010-09-06T06:03:17Z-
dc.date.issued2001en_HK
dc.identifier.citationOsteoarthritis And Cartilage, 2001, v. 9 SUPPL. A, p. S160-S173en_HK
dc.identifier.issn1063-4584en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68301-
dc.description.abstractObjective: Chondrocytes in the growth plate at different stages of differentiation synthesize characteristic extracellular matrix (ECM) components. Mutations in some ECM genes result in chondrodysplasia in humans and mice. We aimed to evaluate the impact of loss- and gain-of-function mutations of ECM genes on matrix structure, gene expression and formation of the growth plate. Design: We review information on the impact of deficiencies in proteoglycans, and types X and II collagens on skeletal development. Additionally, we compare the impact of a glycine904 to cysteine (G904C) mutation in the triple helical coding domain of mouse Col2a1 with two previously reported Col2a1 mutations (exon7 deletion (Del1) and G85C). The G904C Col2a1 gene was introduced as a transgene into mice. Transgenic newborn mice were examined for skeletal development. The histology of the epiphyseal cartilage and the growth plate, and the ultrastructure of chondrocytes and collagen fibrillar morphology in the ECM were studied in 18.5-day transgenic and wild-type fetuses. The distribution of the mRNAs for Col2a1, Col11a1, Col9a1, Matn1, Agc and Ihh in the growth plate of 18.5-day G904C/G904C and wild type fetuses were compared by in situ hybridization. Results: Heterozygous transgenic mice harbouring five copies of the G904C Col2a1 transgene developed skeletal abnormalities and dwarfism. Homozygous G904C/G904C mice died at birth, showing cleft palate, disrupted zonation of chondrocytes and reduction of the zone of hypertrophic chondrocytes. Fewer collagen fibrils were found in ECM of the cartilage. Rough endoplasmic reticulum of the chondrocytes of G904C/ + and G904C/G904C mice was distended. In G904C/G904C mutant mice, Agc gene activity was extended to the hypertrophic zone. Expression of the other genes studied was unchanged. Calcified materials that were not found normally in the maturing and only at low abundance in the hypertrophic zones of the wild type growth plate, were present in these zones in G904C/G904C mice. Despite phenotypic similarities for the G904C and Del1 mice, reduced expression of types I, II, IX, X collagens and aggrecan were reported for the latter mutation. Changes in gene activity and matrix organization in the growth plate also accompanied deficiencies in aggrecan, perlecan and collagen II. Conclusions: The data suggest that a single amino acid alteration in collagen II could lead to skeletal abnormalities through multiple secondary effects on the synthesis and assembly of ECM components. The functional impact of mutations of ECM genes reveals that chondrodysplasia is caused not just by the formation of abnormal matrix molecules, but that the alteration of one ECM component may lead to a cascade of disruption of other gene activities in chondrocytes which collectively contribute to the pathological changes in the architecture of the growth plate. © 2001 OsteoArthritis Research Society International.en_HK
dc.languageengen_HK
dc.publisherWB Saunders Co Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jocaen_HK
dc.relation.ispartofOsteoarthritis and Cartilageen_HK
dc.subject.meshAggrecansen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCartilage, Articular - physiologyen_HK
dc.subject.meshChondrocytes - ultrastructureen_HK
dc.subject.meshCollagen Type I - physiology - ultrastructureen_HK
dc.subject.meshCollagen Type II - physiology - ultrastructureen_HK
dc.subject.meshCollagen Type IX - physiology - ultrastructureen_HK
dc.subject.meshCollagen Type X - physiology - ultrastructureen_HK
dc.subject.meshExtracellular Matrix - physiologyen_HK
dc.subject.meshExtracellular Matrix Proteins - geneticsen_HK
dc.subject.meshGene Expressionen_HK
dc.subject.meshGrowth Plate - anatomy & histologyen_HK
dc.subject.meshIn Situ Hybridizationen_HK
dc.subject.meshLectins, C-Typeen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Transgenicen_HK
dc.subject.meshMutation - geneticsen_HK
dc.subject.meshProteoglycans - physiologyen_HK
dc.titleImpact of mutations of cartilage matrix genes on matrix structure, gene activity and chondrogenesisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1063-4584&volume=9 Suppl A&spage=S160&epage=S173&date=2001&atitle=Impact+of+mutations+of+cartilage+matrix+genes+on+matrix+structure,+gene+activity+and+chondrogenesisen_HK
dc.identifier.emailCheah, KSE:hrmbdkc@hku.hken_HK
dc.identifier.authorityCheah, KSE=rp00342en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid11680681-
dc.identifier.scopuseid_2-s2.0-0034849060en_HK
dc.identifier.hkuros65346en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034849060&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume9en_HK
dc.identifier.issueSUPPL. Aen_HK
dc.identifier.spageS160en_HK
dc.identifier.epageS173en_HK
dc.identifier.isiWOS:000170988600023-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridSo, CL=36979763700en_HK
dc.identifier.scopusauthoridKaluarachchi, K=36905056500en_HK
dc.identifier.scopusauthoridTam, PPL=7202539412en_HK
dc.identifier.scopusauthoridCheah, KSE=35387746200en_HK

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