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Article: Epidermal growth factor induces Gadd45 (growth arrest and DNA damage inducible protein) expression in A431 cells

TitleEpidermal growth factor induces Gadd45 (growth arrest and DNA damage inducible protein) expression in A431 cells
Authors
KeywordsA431 cell
Apoptosis
Epidermal growth factor
Gadd45
Issue Date2001
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbaexp
Citation
Biochimica Et Biophysica Acta - Gene Structure And Expression, 2001, v. 1517 n. 2, p. 250-256 How to Cite?
AbstractEpidermal growth factor (EGF) receptor over-expressing, p53-deficient A431 cells response to toxic dose of EGF by G1 arrest and apoptosis. Applying cDNA expression array technology we demonstrated that EGF increased the levels of Gadd45 mRNA. Northern blot and Western blot analyses confirmed that both Gadd45 mRNA and protein were increased. Concurrently half-lives of Gadd45 mRNA and protein also increased. Nuclear run-on experiments did not show a large increase of Gadd45 mRNA transcription rate. Gadd45 mRNA and protein started to increase after 1 h of EGF treatment and remained high for up to 10 h. We have also confirmed previous studies which showed that in EGF-stimulated A431 cells p21Cip1/Waf1 (cyclin-dependent kinase interacting protein 1) was up-regulated within the same time frame. Thus it appears that in addition to inducing G2 arrest by directly disrupting Cdc2/Cyclin B1 complex in genotoxic-stressed cells, Gadd45 may also participate in G1 arrest in growth factor overexposed cells. © 2001 Elsevier Science B.V.
Persistent Identifierhttp://hdl.handle.net/10722/68299
ISSN
2007 Impact Factor: 1.704
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorFong, WFen_HK
dc.contributor.authorLeung, CHen_HK
dc.contributor.authorLam, Wen_HK
dc.contributor.authorWong, NSen_HK
dc.contributor.authorCheng, SHen_HK
dc.date.accessioned2010-09-06T06:03:16Z-
dc.date.available2010-09-06T06:03:16Z-
dc.date.issued2001en_HK
dc.identifier.citationBiochimica Et Biophysica Acta - Gene Structure And Expression, 2001, v. 1517 n. 2, p. 250-256en_HK
dc.identifier.issn0167-4781en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68299-
dc.description.abstractEpidermal growth factor (EGF) receptor over-expressing, p53-deficient A431 cells response to toxic dose of EGF by G1 arrest and apoptosis. Applying cDNA expression array technology we demonstrated that EGF increased the levels of Gadd45 mRNA. Northern blot and Western blot analyses confirmed that both Gadd45 mRNA and protein were increased. Concurrently half-lives of Gadd45 mRNA and protein also increased. Nuclear run-on experiments did not show a large increase of Gadd45 mRNA transcription rate. Gadd45 mRNA and protein started to increase after 1 h of EGF treatment and remained high for up to 10 h. We have also confirmed previous studies which showed that in EGF-stimulated A431 cells p21Cip1/Waf1 (cyclin-dependent kinase interacting protein 1) was up-regulated within the same time frame. Thus it appears that in addition to inducing G2 arrest by directly disrupting Cdc2/Cyclin B1 complex in genotoxic-stressed cells, Gadd45 may also participate in G1 arrest in growth factor overexposed cells. © 2001 Elsevier Science B.V.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbaexpen_HK
dc.relation.ispartofBiochimica et Biophysica Acta - Gene Structure and Expressionen_HK
dc.rightsBiochimica et Biophysica Acta. Copyright © Elsevier BV.en_HK
dc.subjectA431 cellen_HK
dc.subjectApoptosisen_HK
dc.subjectEpidermal growth factoren_HK
dc.subjectGadd45en_HK
dc.subject.meshBlotting, Westernen_HK
dc.subject.meshCell Cycleen_HK
dc.subject.meshCell Lineen_HK
dc.subject.meshDNA Damageen_HK
dc.subject.meshDNA, Complementary - biosynthesisen_HK
dc.subject.meshEpidermal Growth Factor - pharmacologyen_HK
dc.subject.meshGene Expression Profilingen_HK
dc.subject.meshGene Expression Regulationen_HK
dc.subject.meshHumansen_HK
dc.subject.meshIntracellular Signaling Peptides and Proteinsen_HK
dc.subject.meshProtein Biosynthesisen_HK
dc.subject.meshProteins - geneticsen_HK
dc.subject.meshRNA, Messenger - biosynthesisen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshTumor Cells, Cultureden_HK
dc.titleEpidermal growth factor induces Gadd45 (growth arrest and DNA damage inducible protein) expression in A431 cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-3002&volume=1517&spage=250&epage=256&date=2001&atitle=Epidermal+Growth+Factor+induces+Gadd45+(growth+arrest+and+DNA+damage+inducible+protein)+expression+in+A431+cellsen_HK
dc.identifier.emailLeung, CH:duncanl@hkucc.hku.hken_HK
dc.identifier.emailWong, NS:nswong@hkucc.hku.hken_HK
dc.identifier.authorityLeung, CH=rp00730en_HK
dc.identifier.authorityWong, NS=rp00340en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0167-4781(00)00287-6en_HK
dc.identifier.pmid11342106-
dc.identifier.scopuseid_2-s2.0-0035951665en_HK
dc.identifier.hkuros58816en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035951665&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1517en_HK
dc.identifier.issue2en_HK
dc.identifier.spage250en_HK
dc.identifier.epage256en_HK
dc.identifier.isiWOS:000166849900009-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridFong, WF=7102816013en_HK
dc.identifier.scopusauthoridLeung, CH=7402612570en_HK
dc.identifier.scopusauthoridLam, W=7203021943en_HK
dc.identifier.scopusauthoridWong, NS=7202836641en_HK
dc.identifier.scopusauthoridCheng, SH=20233852300en_HK

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