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Article: Effects of protein kinase modulators on transferrin receptor expression in human leukaemic HL-60 cells

TitleEffects of protein kinase modulators on transferrin receptor expression in human leukaemic HL-60 cells
Authors
KeywordsBisindolylmaleimide (GF109203X)
HL-60
Isoquinolinesulfonamide (H-89)
Protein kinase
Staurosporine
Transferrin receptor
Issue Date1995
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/febslet
Citation
Febs Letters, 1995, v. 365 n. 2-3, p. 137-140 How to Cite?
AbstractThe mRNA of transferrin receptor (TfR) is constitutively expressed in proliferating human leukaemic HL-60 cells. Treatment of HL-60 cells with phorbol 12-myristate 13-acetate (PMA), a protein kinase C (PKC) activator, or dibutyryl-cyclic AMP (dbcAMP), a protein kinase A (PKA) activator, resulted in a 90% decrease in the level of TfR mRNA. Inhibition of TfR mRNA expression induced by 10 nM PMA and 100 μM dbcAMP was abolished by prior incubation of cells with 0.1-1.0 μM GF109203X, a PKC-specific inhibitor, and 1-10 μM H- 89, a PKA-specific inhibitor, respectively. The blocking effects of GF109203X and H-89 were dose-dependent and complete at the highest concentrations of the inhibitors used. Although treatment of cells with GF109203X or H-89 alone did not alter the constitutive expression of TfR mRNA, incubation of cells with 30-100 nM staurosporine, a wide-spectrum protein kinase inhibitor, resulted in suppression of the constitutive expression of TfR mRNA in a dose- dependent manner. These results suggest that (i) the down-regulation of TfR mRNA expression during the differentiation of HL-60 cells can be mediated by activation of either PKC or PKA; (ii) the constitutive expression of TfR mRNA in proliferating HL-60 cells is staurosporine-sensitive and is probably maintained by protein kinase(s) other than PKC and PKA.
Persistent Identifierhttp://hdl.handle.net/10722/68291
ISSN
2015 Impact Factor: 3.519
2015 SCImago Journal Rankings: 2.026
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLok, CNen_HK
dc.contributor.authorChan, KFJen_HK
dc.contributor.authorLoh, TTen_HK
dc.date.accessioned2010-09-06T06:03:11Z-
dc.date.available2010-09-06T06:03:11Z-
dc.date.issued1995en_HK
dc.identifier.citationFebs Letters, 1995, v. 365 n. 2-3, p. 137-140en_HK
dc.identifier.issn0014-5793en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68291-
dc.description.abstractThe mRNA of transferrin receptor (TfR) is constitutively expressed in proliferating human leukaemic HL-60 cells. Treatment of HL-60 cells with phorbol 12-myristate 13-acetate (PMA), a protein kinase C (PKC) activator, or dibutyryl-cyclic AMP (dbcAMP), a protein kinase A (PKA) activator, resulted in a 90% decrease in the level of TfR mRNA. Inhibition of TfR mRNA expression induced by 10 nM PMA and 100 μM dbcAMP was abolished by prior incubation of cells with 0.1-1.0 μM GF109203X, a PKC-specific inhibitor, and 1-10 μM H- 89, a PKA-specific inhibitor, respectively. The blocking effects of GF109203X and H-89 were dose-dependent and complete at the highest concentrations of the inhibitors used. Although treatment of cells with GF109203X or H-89 alone did not alter the constitutive expression of TfR mRNA, incubation of cells with 30-100 nM staurosporine, a wide-spectrum protein kinase inhibitor, resulted in suppression of the constitutive expression of TfR mRNA in a dose- dependent manner. These results suggest that (i) the down-regulation of TfR mRNA expression during the differentiation of HL-60 cells can be mediated by activation of either PKC or PKA; (ii) the constitutive expression of TfR mRNA in proliferating HL-60 cells is staurosporine-sensitive and is probably maintained by protein kinase(s) other than PKC and PKA.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/febsleten_HK
dc.relation.ispartofFEBS Lettersen_HK
dc.rightsF E B S Letters. Copyright © Elsevier BV.en_HK
dc.subjectBisindolylmaleimide (GF109203X)en_HK
dc.subjectHL-60en_HK
dc.subjectIsoquinolinesulfonamide (H-89)en_HK
dc.subjectProtein kinaseen_HK
dc.subjectStaurosporineen_HK
dc.subjectTransferrin receptoren_HK
dc.titleEffects of protein kinase modulators on transferrin receptor expression in human leukaemic HL-60 cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0014-5793&volume=365&spage=137&epage=140&date=1995&atitle=Effects+of+protein+kinase+modulators+on+transferrin+receptor+expression+in+human+leukaemic+HL-60+cellsen_HK
dc.identifier.emailLok, CN: cnlok@hku.hken_HK
dc.identifier.emailChan, KFJ: kfjchan@hkucc.hku.hken_HK
dc.identifier.authorityLok, CN=rp00752en_HK
dc.identifier.authorityChan, KFJ=rp00378en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/0014-5793(95)00442-Cen_HK
dc.identifier.pmid7781766-
dc.identifier.scopuseid_2-s2.0-0029031380en_HK
dc.identifier.hkuros5931en_HK
dc.identifier.volume365en_HK
dc.identifier.issue2-3en_HK
dc.identifier.spage137en_HK
dc.identifier.epage140en_HK
dc.identifier.isiWOS:A1995RB21600008-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLok, CN=7006410829en_HK
dc.identifier.scopusauthoridChan, KFJ=7406035590en_HK
dc.identifier.scopusauthoridLoh, TT=36849142100en_HK

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