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Article: Phosphatidylinositol 3-kinase and protein kinase C are required for the inhibition of caspase activity by epidermal growth factor

TitlePhosphatidylinositol 3-kinase and protein kinase C are required for the inhibition of caspase activity by epidermal growth factor
Authors
Issue Date1999
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/febslet
Citation
Febs Letters, 1999, v. 444 n. 1, p. 90-96 How to Cite?
AbstractThe mechanism by which growth factors exert an anti-apoptotic function on many cell types is not well understood. This issue is addressed in relation to epidermal growth factor (EGF) which inhibits apoptosis induced by staurosporine or wortmannin in an epithelial tumour cell line (CNE-2). The presence of EGF substantially reduced the in vitro Ac-DEVD-AMC hydrolytic activity and almost completely suppressed the intracellular cleavage of poly(ADP-ribose) polymerase in staurosporine- or wortmannin-treated cells. Staurosporine but not wortmannin caused the intracellular proteolytic processing of pro-caspase-3 and this event was transiently inhibited by EGF. Staurosporine-induced apoptosis was not inhibited by EGF in the presence of wortmannin or LY294002. Similarly, EGF failed to inhibit wortmannin-induced apoptosis in the presence of staurosporine, chelerythrine chloride or Go6850. These results suggest that phosphatidylinositol 3-kinase and protein kinase C play a role in the survival function of EGF but the reduction of cellular caspase activity cannot be satisfactorily explained by a lack of pro-caspase-3 activation.
Persistent Identifierhttp://hdl.handle.net/10722/68283
ISSN
2015 Impact Factor: 3.519
2015 SCImago Journal Rankings: 2.026
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLan, Len_HK
dc.contributor.authorWong, NSen_HK
dc.date.accessioned2010-09-06T06:03:06Z-
dc.date.available2010-09-06T06:03:06Z-
dc.date.issued1999en_HK
dc.identifier.citationFebs Letters, 1999, v. 444 n. 1, p. 90-96en_HK
dc.identifier.issn0014-5793en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68283-
dc.description.abstractThe mechanism by which growth factors exert an anti-apoptotic function on many cell types is not well understood. This issue is addressed in relation to epidermal growth factor (EGF) which inhibits apoptosis induced by staurosporine or wortmannin in an epithelial tumour cell line (CNE-2). The presence of EGF substantially reduced the in vitro Ac-DEVD-AMC hydrolytic activity and almost completely suppressed the intracellular cleavage of poly(ADP-ribose) polymerase in staurosporine- or wortmannin-treated cells. Staurosporine but not wortmannin caused the intracellular proteolytic processing of pro-caspase-3 and this event was transiently inhibited by EGF. Staurosporine-induced apoptosis was not inhibited by EGF in the presence of wortmannin or LY294002. Similarly, EGF failed to inhibit wortmannin-induced apoptosis in the presence of staurosporine, chelerythrine chloride or Go6850. These results suggest that phosphatidylinositol 3-kinase and protein kinase C play a role in the survival function of EGF but the reduction of cellular caspase activity cannot be satisfactorily explained by a lack of pro-caspase-3 activation.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/febsleten_HK
dc.relation.ispartofFEBS Lettersen_HK
dc.rightsF E B S Letters. Copyright © Elsevier BV.en_HK
dc.subject.meshAlkaloidsen_HK
dc.subject.meshAndrostadienes - antagonists & inhibitors - pharmacologyen_HK
dc.subject.meshApoptosis - drug effectsen_HK
dc.subject.meshBenzophenanthridinesen_HK
dc.subject.meshBlotting, Westernen_HK
dc.subject.meshCaspase 3en_HK
dc.subject.meshCaspases - antagonists & inhibitors - metabolismen_HK
dc.subject.meshChromatin - drug effects - metabolism - ultrastructureen_HK
dc.subject.meshChromones - pharmacologyen_HK
dc.subject.meshDNA Fragmentation - drug effectsen_HK
dc.subject.meshEnzyme Precursors - metabolismen_HK
dc.subject.meshEpidermal Growth Factor - pharmacologyen_HK
dc.subject.meshFlow Cytometryen_HK
dc.subject.meshHumansen_HK
dc.subject.meshIndoles - pharmacologyen_HK
dc.subject.meshMaleimides - pharmacologyen_HK
dc.subject.meshMolecular Weighten_HK
dc.subject.meshMorpholines - pharmacologyen_HK
dc.subject.meshPhenanthridines - pharmacologyen_HK
dc.subject.meshPhosphatidylinositol 3-Kinases - antagonists & inhibitors - metabolismen_HK
dc.subject.meshPoly(ADP-ribose) Polymerases - metabolismen_HK
dc.subject.meshProtein Kinase C - antagonists & inhibitors - metabolismen_HK
dc.subject.meshStaurosporine - antagonists & inhibitors - pharmacologyen_HK
dc.subject.meshTetradecanoylphorbol Acetate - pharmacologyen_HK
dc.subject.meshTumor Cells, Cultureden_HK
dc.titlePhosphatidylinositol 3-kinase and protein kinase C are required for the inhibition of caspase activity by epidermal growth factoren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0014-5793&volume=444&issue=1&spage=90&epage=96&date=1999&atitle=Phosphatidylinositol+3-kinase+and+protein+kinase+C+are+required+for+the+inhibition+of+caspase+activity+by+epidermal+growth+factoren_HK
dc.identifier.emailWong, NS:nswong@hkucc.hku.hken_HK
dc.identifier.authorityWong, NS=rp00340en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0014-5793(99)00032-0en_HK
dc.identifier.pmid10037154-
dc.identifier.scopuseid_2-s2.0-0033004572en_HK
dc.identifier.hkuros43135en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033004572&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume444en_HK
dc.identifier.issue1en_HK
dc.identifier.spage90en_HK
dc.identifier.epage96en_HK
dc.identifier.isiWOS:000078616200018-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLan, L=36905093300en_HK
dc.identifier.scopusauthoridWong, NS=7202836641en_HK

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