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Article: Axon routing at the optic chiasm after enzymatic removal of chondroitin sulfate in mouse embryos

TitleAxon routing at the optic chiasm after enzymatic removal of chondroitin sulfate in mouse embryos
Authors
Issue Date2000
PublisherThe Company of Biologists Ltd. The Journal's web site is located at http://dev.biologists.org
Citation
Development, 2000, v. 127 n. 12, p. 2673-2683 How to Cite?
AbstractThe effects of removing chondroitin sulfate from chondroitin sulfate proteoglycan molecules on guidance of retinal ganglion cell axons at the optic chiasm were investigated in a brain slice preparation of mouse embryos of embryonic day 13 to 15. Slices were grown for 5 hours and growth of dye-labeled axons was traced through the chiasm. After continuous enzymatic digestion of the chondroitin sulfate proteoglycans with chondroitinase ABC, which removes the glycosaminoglycan chains, navigation of retinal axons was disrupted. At embryonic day 13, before the uncrossed projection forms in normal development, many axons deviated from their normal course, crossing the midline at aberrant positions and invading the ventral diencephalon. In slices from embryonic day 14 embryos, axons that would normally form the uncrossed projection at this stage failed to turn into the ipsilateral optic tract. In embryonic day 15 slices, enzyme treatment caused a reduction of the uncrossed projection that develops at this stage. Growth cones in enzyme-treated slices showed a significant increase in the size both before and after they crossed the midline. This indicates that responses of retinal axons to guidance signals at the chiasm have changed after removal of the chondroitin sulfate epitope. We concluded that the chondroitin sulfate moieties of the proteoglycans are involved in patterning the early phase of axonal growth across the midline and at a later stage controlling the axon divergence at the chiasm.
Persistent Identifierhttp://hdl.handle.net/10722/68280
ISSN
2015 Impact Factor: 6.059
2015 SCImago Journal Rankings: 5.239
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChung, KYen_HK
dc.contributor.authorTaylor, JSHen_HK
dc.contributor.authorShum, DKYen_HK
dc.contributor.authorChan, SOen_HK
dc.date.accessioned2010-09-06T06:03:05Z-
dc.date.available2010-09-06T06:03:05Z-
dc.date.issued2000en_HK
dc.identifier.citationDevelopment, 2000, v. 127 n. 12, p. 2673-2683en_HK
dc.identifier.issn0950-1991en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68280-
dc.description.abstractThe effects of removing chondroitin sulfate from chondroitin sulfate proteoglycan molecules on guidance of retinal ganglion cell axons at the optic chiasm were investigated in a brain slice preparation of mouse embryos of embryonic day 13 to 15. Slices were grown for 5 hours and growth of dye-labeled axons was traced through the chiasm. After continuous enzymatic digestion of the chondroitin sulfate proteoglycans with chondroitinase ABC, which removes the glycosaminoglycan chains, navigation of retinal axons was disrupted. At embryonic day 13, before the uncrossed projection forms in normal development, many axons deviated from their normal course, crossing the midline at aberrant positions and invading the ventral diencephalon. In slices from embryonic day 14 embryos, axons that would normally form the uncrossed projection at this stage failed to turn into the ipsilateral optic tract. In embryonic day 15 slices, enzyme treatment caused a reduction of the uncrossed projection that develops at this stage. Growth cones in enzyme-treated slices showed a significant increase in the size both before and after they crossed the midline. This indicates that responses of retinal axons to guidance signals at the chiasm have changed after removal of the chondroitin sulfate epitope. We concluded that the chondroitin sulfate moieties of the proteoglycans are involved in patterning the early phase of axonal growth across the midline and at a later stage controlling the axon divergence at the chiasm.en_HK
dc.languageengen_HK
dc.publisherThe Company of Biologists Ltd. The Journal's web site is located at http://dev.biologists.orgen_HK
dc.relation.ispartofDevelopmenten_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAxons - physiologyen_HK
dc.subject.meshChondroitin ABC Lyaseen_HK
dc.subject.meshChondroitin Sulfate Proteoglycans - physiologyen_HK
dc.subject.meshChondroitin Sulfates - physiologyen_HK
dc.subject.meshDiencephalon - embryologyen_HK
dc.subject.meshEmbryonic and Fetal Developmenten_HK
dc.subject.meshGestational Ageen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Inbred C57BLen_HK
dc.subject.meshOptic Chiasm - embryologyen_HK
dc.subject.meshRetinal Ganglion Cells - physiologyen_HK
dc.titleAxon routing at the optic chiasm after enzymatic removal of chondroitin sulfate in mouse embryosen_HK
dc.typeArticleen_HK
dc.identifier.emailShum, DKY:shumdkhk@hkucc.hku.hken_HK
dc.identifier.authorityShum, DKY=rp00321en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.pmid10821765-
dc.identifier.scopuseid_2-s2.0-0033917707en_HK
dc.identifier.hkuros53307en_HK
dc.identifier.hkuros61135-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033917707&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume127en_HK
dc.identifier.issue12en_HK
dc.identifier.spage2673en_HK
dc.identifier.epage2683en_HK
dc.identifier.isiWOS:000087948900015-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridChung, KY=7404085795en_HK
dc.identifier.scopusauthoridTaylor, JSH=35571124800en_HK
dc.identifier.scopusauthoridShum, DKY=7004824447en_HK
dc.identifier.scopusauthoridChan, SO=7404256181en_HK

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