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Article: Overexpression of soluble TRAIL induces apoptosis in human lung adenocarcinoma and inhibits growth of tumor xenografts in nude mice

TitleOverexpression of soluble TRAIL induces apoptosis in human lung adenocarcinoma and inhibits growth of tumor xenografts in nude mice
Authors
Issue Date2005
PublisherAmerican Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/
Citation
Cancer Research, 2005, v. 65 n. 5, p. 1687-1692 How to Cite?
AbstractRecombinant adeno-associated virus 2/5 (rAAV2/5), a hybrid rAAV-2 with AAV-5 capsid, seems to be a very efficient delivery vector for the transduction of the lung adenocarcinoma cell line A549. Infection of the A549 cell line with a rAAV2/5 vector encoding the extracellular domain of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL, amino acids 114-281) resulted in secretion of soluble TRAIL (sTRAIL) and induction of apoptosis in these cells. rAAV2/5-sTRAIL mediated delivery and stable expression of sTRAIL resulted in the presence of the trimeric form of sTRAIL in sera of nude mice that were implanted with s.c. or orthotopic A549 tumors. The rAAV2/5-sTRAIL transduction of the tumors resulted in a statistically significant reduction in tumor growth and prolonged survival of the tumor-bearing animals. Primary cell culture, histologic examination of the tumors, and serum analyses showed the absence of detectable TRAIL-induced toxicity in normal tissues including the liver. The successful inhibition of lung cancer growth and the absence of detectable toxicity suggest a putative role for rAAV2/5-sTRAIL114.281 in the therapy of lung cancer. ©2005 American Association for Cancer Research.
Persistent Identifierhttp://hdl.handle.net/10722/68279
ISSN
2015 Impact Factor: 8.556
2015 SCImago Journal Rankings: 5.372
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorShi, Jen_HK
dc.contributor.authorZheng, Den_HK
dc.contributor.authorLiu, Yen_HK
dc.contributor.authorMai, HSen_HK
dc.contributor.authorTam, Pen_HK
dc.contributor.authorFarzaneh, Fen_HK
dc.contributor.authorXu, Ren_HK
dc.date.accessioned2010-09-06T06:03:04Z-
dc.date.available2010-09-06T06:03:04Z-
dc.date.issued2005en_HK
dc.identifier.citationCancer Research, 2005, v. 65 n. 5, p. 1687-1692en_HK
dc.identifier.issn0008-5472en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68279-
dc.description.abstractRecombinant adeno-associated virus 2/5 (rAAV2/5), a hybrid rAAV-2 with AAV-5 capsid, seems to be a very efficient delivery vector for the transduction of the lung adenocarcinoma cell line A549. Infection of the A549 cell line with a rAAV2/5 vector encoding the extracellular domain of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL, amino acids 114-281) resulted in secretion of soluble TRAIL (sTRAIL) and induction of apoptosis in these cells. rAAV2/5-sTRAIL mediated delivery and stable expression of sTRAIL resulted in the presence of the trimeric form of sTRAIL in sera of nude mice that were implanted with s.c. or orthotopic A549 tumors. The rAAV2/5-sTRAIL transduction of the tumors resulted in a statistically significant reduction in tumor growth and prolonged survival of the tumor-bearing animals. Primary cell culture, histologic examination of the tumors, and serum analyses showed the absence of detectable TRAIL-induced toxicity in normal tissues including the liver. The successful inhibition of lung cancer growth and the absence of detectable toxicity suggest a putative role for rAAV2/5-sTRAIL114.281 in the therapy of lung cancer. ©2005 American Association for Cancer Research.en_HK
dc.languageengen_HK
dc.publisherAmerican Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/en_HK
dc.relation.ispartofCancer Researchen_HK
dc.subject.meshAdenocarcinoma - pathology - therapyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshApoptosisen_HK
dc.subject.meshApoptosis Regulatory Proteinsen_HK
dc.subject.meshDependovirus - geneticsen_HK
dc.subject.meshGene Therapyen_HK
dc.subject.meshGreen Fluorescent Proteins - metabolismen_HK
dc.subject.meshHumansen_HK
dc.subject.meshLiver - drug effectsen_HK
dc.subject.meshLung Neoplasms - pathology - therapyen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMembrane Glycoproteins - genetics - metabolismen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Inbred BALB Cen_HK
dc.subject.meshMice, Nudeen_HK
dc.subject.meshSurvival Rateen_HK
dc.subject.meshTNF-Related Apoptosis-Inducing Liganden_HK
dc.subject.meshTransduction, Geneticen_HK
dc.subject.meshTransfectionen_HK
dc.subject.meshTransplantation, Heterologousen_HK
dc.subject.meshTumor Cells, Cultureden_HK
dc.subject.meshTumor Necrosis Factor-alpha - genetics - metabolismen_HK
dc.titleOverexpression of soluble TRAIL induces apoptosis in human lung adenocarcinoma and inhibits growth of tumor xenografts in nude miceen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0008-5472&volume=65&issue=5&spage=1687&epage=1692&date=2005&atitle=Overexpression+of+soluble+TRAIL+induces+apoptosis+in+human+lung+adenocarcinoma+and+inhibits+growth+of+tumor+xenografts+in+nude+mice.en_HK
dc.identifier.emailMai, HS:mhsham@hkucc.hku.hken_HK
dc.identifier.emailTam, P:paultam@hkucc.hku.hken_HK
dc.identifier.authorityMai, HS=rp00380en_HK
dc.identifier.authorityTam, P=rp00060en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1158/0008-5472.CAN-04-2749en_HK
dc.identifier.pmid15753363-
dc.identifier.scopuseid_2-s2.0-16444381759en_HK
dc.identifier.hkuros103174en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-16444381759&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume65en_HK
dc.identifier.issue5en_HK
dc.identifier.spage1687en_HK
dc.identifier.epage1692en_HK
dc.identifier.isiWOS:000227294600011-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridShi, J=7404495444en_HK
dc.identifier.scopusauthoridZheng, D=7202567084en_HK
dc.identifier.scopusauthoridLiu, Y=8232975800en_HK
dc.identifier.scopusauthoridMai, HS=7003729109en_HK
dc.identifier.scopusauthoridTam, P=7202539421en_HK
dc.identifier.scopusauthoridFarzaneh, F=7006545549en_HK
dc.identifier.scopusauthoridXu, R=7402813857en_HK

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