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Article: Developmental expression of NMDA and AMPA receptor subunits in vestibular nuclear neurons that encode gravity-related horizontal orientations

TitleDevelopmental expression of NMDA and AMPA receptor subunits in vestibular nuclear neurons that encode gravity-related horizontal orientations
Authors
Keywordsc-fos
Glutamate receptor
Otolith organ
Vestibular nucleus
Issue Date2008
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31248
Citation
Journal Of Comparative Neurology, 2008, v. 508 n. 2, p. 343-364 How to Cite?
AbstractWe examined the expression profile of subunits of ionotropic glutamate receptors [N-methyl-D-aspartate (NMDA) and α-amino-3-hydroxy-5-methyl-4- isoxazole-proprionate (AMPA)] during postnatal development of connectivity in the rat vestibular nucleus. Vestibular nuclear neurons were functionally activated by constant velocity off-vertical axis rotation, a strategy to stimulate otolith organs in the inner ear. These neurons indicated Fos expression as a result. By immunodetection for Fos, otolith-related neurons that expressed NMDA/AMPA receptor subunits were identified as early as P7, and these neurons were found to increase progressively up to adulthood. Although there was developmental invariance in the percentage of Fos-immunoreactive neurons expressing the NR1, NR2A, GluR1, or GluR2/3 subunits, those expressing the NR2B subunit decreased from P14 onward, and those expressing the GluR4 subunit decreased in adults. These double-immunohistochemical data were corroborated by combined immuno-/hybridization histochemical data obtained from Fos-immunoreactive neurons expressing NR2B mRNA or GluR4 mRNA. The staining of both NR2B and GluR4 in the cytoplasm of these neurons decreased upon maturation. The percentage of Fos-immunoreactive neurons expressing the other ionotropic glutamate receptor subunits (viz. NR1, NR2A, GluR1, and GluR2/3) remained relatively constant throughout postnatal maturation. Triple immunofluorescence further demonstrated coexpression of NR1 and NR2 subunits in Fos-immunoreactive neurons. Coexpression of NR1 subunit with each of the GluR subunits was also observed among the Fos-immunoreactive neurons. Taken together, the different expression profiles of ionotropic glutamate receptor subunits constitute the histological basis for glutamatergic neurotransmission in the maturation of central vestibular connectivity for the coding of gravity-related horizontal head movements. © 2008 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/68263
ISSN
2015 Impact Factor: 3.331
2015 SCImago Journal Rankings: 2.345
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTse, YCen_HK
dc.contributor.authorLai, CHen_HK
dc.contributor.authorLai, SKen_HK
dc.contributor.authorLiu, JXen_HK
dc.contributor.authorYung, KKLen_HK
dc.contributor.authorShum, DKYen_HK
dc.contributor.authorChan, YSen_HK
dc.date.accessioned2010-09-06T06:02:54Z-
dc.date.available2010-09-06T06:02:54Z-
dc.date.issued2008en_HK
dc.identifier.citationJournal Of Comparative Neurology, 2008, v. 508 n. 2, p. 343-364en_HK
dc.identifier.issn0021-9967en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68263-
dc.description.abstractWe examined the expression profile of subunits of ionotropic glutamate receptors [N-methyl-D-aspartate (NMDA) and α-amino-3-hydroxy-5-methyl-4- isoxazole-proprionate (AMPA)] during postnatal development of connectivity in the rat vestibular nucleus. Vestibular nuclear neurons were functionally activated by constant velocity off-vertical axis rotation, a strategy to stimulate otolith organs in the inner ear. These neurons indicated Fos expression as a result. By immunodetection for Fos, otolith-related neurons that expressed NMDA/AMPA receptor subunits were identified as early as P7, and these neurons were found to increase progressively up to adulthood. Although there was developmental invariance in the percentage of Fos-immunoreactive neurons expressing the NR1, NR2A, GluR1, or GluR2/3 subunits, those expressing the NR2B subunit decreased from P14 onward, and those expressing the GluR4 subunit decreased in adults. These double-immunohistochemical data were corroborated by combined immuno-/hybridization histochemical data obtained from Fos-immunoreactive neurons expressing NR2B mRNA or GluR4 mRNA. The staining of both NR2B and GluR4 in the cytoplasm of these neurons decreased upon maturation. The percentage of Fos-immunoreactive neurons expressing the other ionotropic glutamate receptor subunits (viz. NR1, NR2A, GluR1, and GluR2/3) remained relatively constant throughout postnatal maturation. Triple immunofluorescence further demonstrated coexpression of NR1 and NR2 subunits in Fos-immunoreactive neurons. Coexpression of NR1 subunit with each of the GluR subunits was also observed among the Fos-immunoreactive neurons. Taken together, the different expression profiles of ionotropic glutamate receptor subunits constitute the histological basis for glutamatergic neurotransmission in the maturation of central vestibular connectivity for the coding of gravity-related horizontal head movements. © 2008 Wiley-Liss, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31248en_HK
dc.relation.ispartofJournal of Comparative Neurologyen_HK
dc.subjectc-fosen_HK
dc.subjectGlutamate receptoren_HK
dc.subjectOtolith organen_HK
dc.subjectVestibular nucleusen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAnimals, Newbornen_HK
dc.subject.meshCell Count - methodsen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGene Expression Regulation, Developmental - physiologyen_HK
dc.subject.meshMaleen_HK
dc.subject.meshNeurons - physiologyen_HK
dc.subject.meshOncogene Proteins v-fos - genetics - metabolismen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.subject.meshReceptors, AMPA - genetics - metabolismen_HK
dc.subject.meshReceptors, N-Methyl-D-Aspartate - genetics - metabolismen_HK
dc.subject.meshVestibular Nuclei - cytologyen_HK
dc.titleDevelopmental expression of NMDA and AMPA receptor subunits in vestibular nuclear neurons that encode gravity-related horizontal orientationsen_HK
dc.typeArticleen_HK
dc.identifier.emailLai, CH: chlaib@hku.hken_HK
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hken_HK
dc.identifier.emailChan, YS: yschan@hkucc.hku.hken_HK
dc.identifier.authorityLai, CH=rp00396en_HK
dc.identifier.authorityShum, DKY=rp00321en_HK
dc.identifier.authorityChan, YS=rp00318en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/cne.21688en_HK
dc.identifier.pmid18335497-
dc.identifier.scopuseid_2-s2.0-42449142688en_HK
dc.identifier.hkuros149589en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-42449142688&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume508en_HK
dc.identifier.issue2en_HK
dc.identifier.spage343en_HK
dc.identifier.epage364en_HK
dc.identifier.eissn1096-9861-
dc.identifier.isiWOS:000254963600009-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridTse, YC=7005116869en_HK
dc.identifier.scopusauthoridLai, CH=7403086597en_HK
dc.identifier.scopusauthoridLai, SK=7402937165en_HK
dc.identifier.scopusauthoridLiu, JX=8345637700en_HK
dc.identifier.scopusauthoridYung, KKL=13605496000en_HK
dc.identifier.scopusauthoridShum, DKY=7004824447en_HK
dc.identifier.scopusauthoridChan, YS=7403676627en_HK

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