Article: 3′ Splicing variants of ret receptor tyrosine kinase are differentially expressed in mouse embryos and in adult mice

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Title3′ Splicing variants of ret receptor tyrosine kinase are differentially expressed in mouse embryos and in adult mice
AuthorsLee, KY1
Samy, ET1 2
Sham, MH1
Tam, PKH1
Lui, VCH1
Keywords3′ Splicing variants
Differential expression
Embryogenesis
Ret protooncogene
Issue Date2003
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbaexp
CitationBiochimica Et Biophysica Acta - Gene Structure And Expression, 2003, v. 1627 n. 1, p. 26-38 [How to Cite?]
DOI: http://dx.doi.org/10.1016/S0167-4781(03)00068-X
AbstractThe RET protooncogene encodes for a transmembrane receptor tyrosine kinase and plays a crucial role in nephrogenesis and the enteric nervous system (ENS) development. Alternative splicing at the 3′ end of the RET gene generates 3′ splicing variants that encode RET 9, RET 51 and RET 43 isoforms. It has been hypothesized that these isoforms perform distinct functions and that their expressions are differentially regulated during mammalian development. To gain an insight into the expression patterns of various ret isoforms during embryogenesis, we investigate the temporal and spatial expressions of ret gene in mouse embryos and in adult mice. We characterized the 3′ end of the mouse ret gene and localized the alternatively spliced exons. Using 3′ rapid amplification of cDNA ends (3′ RACE) and reverse transcription-polymerase chain reaction (RT-PCR), ret 9 and ret 51 transcripts were identified in both mouse embryos and adult mouse tissues. However, the ret 43 transcript was not. Using in situ hybridization, we showed that ret 9 was the dominant ret encoding transcript in mouse embryos. Transcripts of ret 9 were detected in all cranial ganglia; in the sensory and autonomic ganglia of the trunk; in a subset of neurons of the dorsal root ganglion (DRG); in the motor neurons of the spinal cord; in the developing lung and excretory systems; in the enteric neuroblasts of the ENS; and in the thyroid lobes. In contrast, ret 51 expression was weak and restricted to the motor column of the spinal cord, the DRG, the enteric neuroblasts, the lung bud and the kidney. In adult mice, ret 9 expression was relatively widespread in many organs while that of ret 51 was rather restricted. Our data indicated that ret isoforms are temporally and spatially regulated in mouse embryos and adult mouse. © 2003 Elsevier Science B.V. All rights reserved.
ISSN0167-4781
DOIhttp://dx.doi.org/10.1016/S0167-4781(03)00068-X
ISI Accession Number IDWOS:000183223600004
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorLee, KY
dc.contributor.authorSamy, ET
dc.contributor.authorSham, MH
dc.contributor.authorTam, PKH
dc.contributor.authorLui, VCH
dc.date.accessioned2010-09-06T06:02:48Z
dc.date.available2010-09-06T06:02:48Z
dc.date.issued2003
dc.description.abstractThe RET protooncogene encodes for a transmembrane receptor tyrosine kinase and plays a crucial role in nephrogenesis and the enteric nervous system (ENS) development. Alternative splicing at the 3′ end of the RET gene generates 3′ splicing variants that encode RET 9, RET 51 and RET 43 isoforms. It has been hypothesized that these isoforms perform distinct functions and that their expressions are differentially regulated during mammalian development. To gain an insight into the expression patterns of various ret isoforms during embryogenesis, we investigate the temporal and spatial expressions of ret gene in mouse embryos and in adult mice. We characterized the 3′ end of the mouse ret gene and localized the alternatively spliced exons. Using 3′ rapid amplification of cDNA ends (3′ RACE) and reverse transcription-polymerase chain reaction (RT-PCR), ret 9 and ret 51 transcripts were identified in both mouse embryos and adult mouse tissues. However, the ret 43 transcript was not. Using in situ hybridization, we showed that ret 9 was the dominant ret encoding transcript in mouse embryos. Transcripts of ret 9 were detected in all cranial ganglia; in the sensory and autonomic ganglia of the trunk; in a subset of neurons of the dorsal root ganglion (DRG); in the motor neurons of the spinal cord; in the developing lung and excretory systems; in the enteric neuroblasts of the ENS; and in the thyroid lobes. In contrast, ret 51 expression was weak and restricted to the motor column of the spinal cord, the DRG, the enteric neuroblasts, the lung bud and the kidney. In adult mice, ret 9 expression was relatively widespread in many organs while that of ret 51 was rather restricted. Our data indicated that ret isoforms are temporally and spatially regulated in mouse embryos and adult mouse. © 2003 Elsevier Science B.V. All rights reserved.
dc.description.naturelink_to_subscribed_fulltext
dc.identifier.citationBiochimica Et Biophysica Acta - Gene Structure And Expression, 2003, v. 1627 n. 1, p. 26-38 [How to Cite?]
DOI: http://dx.doi.org/10.1016/S0167-4781(03)00068-X
dc.identifier.doihttp://dx.doi.org/10.1016/S0167-4781(03)00068-X
dc.identifier.epage38
dc.identifier.hkuros88339
dc.identifier.isiWOS:000183223600004
dc.identifier.issn0167-4781
dc.identifier.issue1
dc.identifier.openurl
dc.identifier.pmid12759189
dc.identifier.scopuseid_2-s2.0-0038004062
dc.identifier.spage26
dc.identifier.urihttp://hdl.handle.net/10722/68251
dc.identifier.volume1627
dc.languageeng
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbaexp
dc.publisher.placeNetherlands
dc.relation.ispartofBiochimica et Biophysica Acta - Gene Structure and Expression
dc.relation.referencesReferences in Scopus
dc.rightsB B A - Gene Structure and Expression. Copyright © Elsevier BV.
dc.subject.meshAlternative Splicing
dc.subject.meshAmino Acid Sequence
dc.subject.meshAnimals
dc.subject.meshBase Sequence
dc.subject.meshIn Situ Hybridization
dc.subject.meshMice
dc.subject.meshMolecular Sequence Data
dc.subject.meshOrgan Specificity
dc.subject.meshProtein Isoforms - genetics
dc.subject.meshProto-Oncogene Proteins - genetics - metabolism
dc.subject.meshProto-Oncogene Proteins c-ret
dc.subject.meshRNA, Messenger - metabolism
dc.subject.meshReceptor Protein-Tyrosine Kinases - genetics - metabolism
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction
dc.subject3′ Splicing variants
dc.subjectDifferential expression
dc.subjectEmbryogenesis
dc.subjectRet protooncogene
dc.title3′ Splicing variants of ret receptor tyrosine kinase are differentially expressed in mouse embryos and in adult mice
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. University of Virginia Medical School