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Article: 3′ Splicing variants of ret receptor tyrosine kinase are differentially expressed in mouse embryos and in adult mice

Title3′ Splicing variants of ret receptor tyrosine kinase are differentially expressed in mouse embryos and in adult mice
Authors
Keywords3′ Splicing variants
Differential expression
Embryogenesis
Ret protooncogene
Issue Date2003
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbaexp
Citation
Biochimica Et Biophysica Acta - Gene Structure And Expression, 2003, v. 1627 n. 1, p. 26-38 How to Cite?
Abstract
The RET protooncogene encodes for a transmembrane receptor tyrosine kinase and plays a crucial role in nephrogenesis and the enteric nervous system (ENS) development. Alternative splicing at the 3′ end of the RET gene generates 3′ splicing variants that encode RET 9, RET 51 and RET 43 isoforms. It has been hypothesized that these isoforms perform distinct functions and that their expressions are differentially regulated during mammalian development. To gain an insight into the expression patterns of various ret isoforms during embryogenesis, we investigate the temporal and spatial expressions of ret gene in mouse embryos and in adult mice. We characterized the 3′ end of the mouse ret gene and localized the alternatively spliced exons. Using 3′ rapid amplification of cDNA ends (3′ RACE) and reverse transcription-polymerase chain reaction (RT-PCR), ret 9 and ret 51 transcripts were identified in both mouse embryos and adult mouse tissues. However, the ret 43 transcript was not. Using in situ hybridization, we showed that ret 9 was the dominant ret encoding transcript in mouse embryos. Transcripts of ret 9 were detected in all cranial ganglia; in the sensory and autonomic ganglia of the trunk; in a subset of neurons of the dorsal root ganglion (DRG); in the motor neurons of the spinal cord; in the developing lung and excretory systems; in the enteric neuroblasts of the ENS; and in the thyroid lobes. In contrast, ret 51 expression was weak and restricted to the motor column of the spinal cord, the DRG, the enteric neuroblasts, the lung bud and the kidney. In adult mice, ret 9 expression was relatively widespread in many organs while that of ret 51 was rather restricted. Our data indicated that ret isoforms are temporally and spatially regulated in mouse embryos and adult mouse. © 2003 Elsevier Science B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/68251
ISSN
ISI Accession Number ID
References

 

Author Affiliations
  1. The University of Hong Kong
  2. University of Virginia Medical School
DC FieldValueLanguage
dc.contributor.authorLee, KYen_HK
dc.contributor.authorSamy, ETen_HK
dc.contributor.authorSham, MHen_HK
dc.contributor.authorTam, PKHen_HK
dc.contributor.authorLui, VCHen_HK
dc.date.accessioned2010-09-06T06:02:48Z-
dc.date.available2010-09-06T06:02:48Z-
dc.date.issued2003en_HK
dc.identifier.citationBiochimica Et Biophysica Acta - Gene Structure And Expression, 2003, v. 1627 n. 1, p. 26-38en_HK
dc.identifier.issn0167-4781en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68251-
dc.description.abstractThe RET protooncogene encodes for a transmembrane receptor tyrosine kinase and plays a crucial role in nephrogenesis and the enteric nervous system (ENS) development. Alternative splicing at the 3′ end of the RET gene generates 3′ splicing variants that encode RET 9, RET 51 and RET 43 isoforms. It has been hypothesized that these isoforms perform distinct functions and that their expressions are differentially regulated during mammalian development. To gain an insight into the expression patterns of various ret isoforms during embryogenesis, we investigate the temporal and spatial expressions of ret gene in mouse embryos and in adult mice. We characterized the 3′ end of the mouse ret gene and localized the alternatively spliced exons. Using 3′ rapid amplification of cDNA ends (3′ RACE) and reverse transcription-polymerase chain reaction (RT-PCR), ret 9 and ret 51 transcripts were identified in both mouse embryos and adult mouse tissues. However, the ret 43 transcript was not. Using in situ hybridization, we showed that ret 9 was the dominant ret encoding transcript in mouse embryos. Transcripts of ret 9 were detected in all cranial ganglia; in the sensory and autonomic ganglia of the trunk; in a subset of neurons of the dorsal root ganglion (DRG); in the motor neurons of the spinal cord; in the developing lung and excretory systems; in the enteric neuroblasts of the ENS; and in the thyroid lobes. In contrast, ret 51 expression was weak and restricted to the motor column of the spinal cord, the DRG, the enteric neuroblasts, the lung bud and the kidney. In adult mice, ret 9 expression was relatively widespread in many organs while that of ret 51 was rather restricted. Our data indicated that ret isoforms are temporally and spatially regulated in mouse embryos and adult mouse. © 2003 Elsevier Science B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbaexpen_HK
dc.relation.ispartofBiochimica et Biophysica Acta - Gene Structure and Expressionen_HK
dc.rightsB B A - Gene Structure and Expression. Copyright © Elsevier BV.en_HK
dc.subject3′ Splicing variantsen_HK
dc.subjectDifferential expressionen_HK
dc.subjectEmbryogenesisen_HK
dc.subjectRet protooncogeneen_HK
dc.subject.meshAlternative Splicingen_HK
dc.subject.meshAmino Acid Sequenceen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshIn Situ Hybridizationen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshOrgan Specificityen_HK
dc.subject.meshProtein Isoforms - geneticsen_HK
dc.subject.meshProto-Oncogene Proteins - genetics - metabolismen_HK
dc.subject.meshProto-Oncogene Proteins c-reten_HK
dc.subject.meshRNA, Messenger - metabolismen_HK
dc.subject.meshReceptor Protein-Tyrosine Kinases - genetics - metabolismen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.title3′ Splicing variants of ret receptor tyrosine kinase are differentially expressed in mouse embryos and in adult miceen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0167-4781&volume=1627&spage=26&epage=38&date=2003&atitle=3%27+Splicing+variants+of+ret+receptor+tyrosine+kinase+are+differentially+expressed+in+mouse+embryos+and+in+adult+miceen_HK
dc.identifier.emailSham, MH: mhsham@hkucc.hku.hken_HK
dc.identifier.emailTam, PKH: paultam@hkucc.hku.hken_HK
dc.identifier.emailLui, VCH: vchlui@hkucc.hku.hken_HK
dc.identifier.authoritySham, MH=rp00380en_HK
dc.identifier.authorityTam, PKH=rp00060en_HK
dc.identifier.authorityLui, VCH=rp00363en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0167-4781(03)00068-Xen_HK
dc.identifier.pmid12759189en_HK
dc.identifier.scopuseid_2-s2.0-0038004062en_HK
dc.identifier.hkuros88339en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0038004062&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1627en_HK
dc.identifier.issue1en_HK
dc.identifier.spage26en_HK
dc.identifier.epage38en_HK
dc.identifier.isiWOS:000183223600004-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLee, KY=10340983900en_HK
dc.identifier.scopusauthoridSamy, ET=6602891563en_HK
dc.identifier.scopusauthoridSham, MH=7003729109en_HK
dc.identifier.scopusauthoridTam, PKH=7202539421en_HK
dc.identifier.scopusauthoridLui, VCH=7004231344en_HK

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