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Article: 3′ Splicing variants of ret receptor tyrosine kinase are differentially expressed in mouse embryos and in adult mice
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Title3′ Splicing variants of ret receptor tyrosine kinase are differentially expressed in mouse embryos and in adult mice
 
AuthorsLee, KY1
Samy, ET1 2
Sham, MH1
Tam, PKH1
Lui, VCH1
 
Keywords3′ Splicing variants
Differential expression
Embryogenesis
Ret protooncogene
 
Issue Date2003
 
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbaexp
 
CitationBiochimica Et Biophysica Acta - Gene Structure And Expression, 2003, v. 1627 n. 1, p. 26-38 [How to Cite?]
DOI: http://dx.doi.org/10.1016/S0167-4781(03)00068-X
 
AbstractThe RET protooncogene encodes for a transmembrane receptor tyrosine kinase and plays a crucial role in nephrogenesis and the enteric nervous system (ENS) development. Alternative splicing at the 3′ end of the RET gene generates 3′ splicing variants that encode RET 9, RET 51 and RET 43 isoforms. It has been hypothesized that these isoforms perform distinct functions and that their expressions are differentially regulated during mammalian development. To gain an insight into the expression patterns of various ret isoforms during embryogenesis, we investigate the temporal and spatial expressions of ret gene in mouse embryos and in adult mice. We characterized the 3′ end of the mouse ret gene and localized the alternatively spliced exons. Using 3′ rapid amplification of cDNA ends (3′ RACE) and reverse transcription-polymerase chain reaction (RT-PCR), ret 9 and ret 51 transcripts were identified in both mouse embryos and adult mouse tissues. However, the ret 43 transcript was not. Using in situ hybridization, we showed that ret 9 was the dominant ret encoding transcript in mouse embryos. Transcripts of ret 9 were detected in all cranial ganglia; in the sensory and autonomic ganglia of the trunk; in a subset of neurons of the dorsal root ganglion (DRG); in the motor neurons of the spinal cord; in the developing lung and excretory systems; in the enteric neuroblasts of the ENS; and in the thyroid lobes. In contrast, ret 51 expression was weak and restricted to the motor column of the spinal cord, the DRG, the enteric neuroblasts, the lung bud and the kidney. In adult mice, ret 9 expression was relatively widespread in many organs while that of ret 51 was rather restricted. Our data indicated that ret isoforms are temporally and spatially regulated in mouse embryos and adult mouse. © 2003 Elsevier Science B.V. All rights reserved.
 
ISSN0167-4781
 
DOIhttp://dx.doi.org/10.1016/S0167-4781(03)00068-X
 
ISI Accession Number IDWOS:000183223600004
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorLee, KY
 
dc.contributor.authorSamy, ET
 
dc.contributor.authorSham, MH
 
dc.contributor.authorTam, PKH
 
dc.contributor.authorLui, VCH
 
dc.date.accessioned2010-09-06T06:02:48Z
 
dc.date.available2010-09-06T06:02:48Z
 
dc.date.issued2003
 
dc.description.abstractThe RET protooncogene encodes for a transmembrane receptor tyrosine kinase and plays a crucial role in nephrogenesis and the enteric nervous system (ENS) development. Alternative splicing at the 3′ end of the RET gene generates 3′ splicing variants that encode RET 9, RET 51 and RET 43 isoforms. It has been hypothesized that these isoforms perform distinct functions and that their expressions are differentially regulated during mammalian development. To gain an insight into the expression patterns of various ret isoforms during embryogenesis, we investigate the temporal and spatial expressions of ret gene in mouse embryos and in adult mice. We characterized the 3′ end of the mouse ret gene and localized the alternatively spliced exons. Using 3′ rapid amplification of cDNA ends (3′ RACE) and reverse transcription-polymerase chain reaction (RT-PCR), ret 9 and ret 51 transcripts were identified in both mouse embryos and adult mouse tissues. However, the ret 43 transcript was not. Using in situ hybridization, we showed that ret 9 was the dominant ret encoding transcript in mouse embryos. Transcripts of ret 9 were detected in all cranial ganglia; in the sensory and autonomic ganglia of the trunk; in a subset of neurons of the dorsal root ganglion (DRG); in the motor neurons of the spinal cord; in the developing lung and excretory systems; in the enteric neuroblasts of the ENS; and in the thyroid lobes. In contrast, ret 51 expression was weak and restricted to the motor column of the spinal cord, the DRG, the enteric neuroblasts, the lung bud and the kidney. In adult mice, ret 9 expression was relatively widespread in many organs while that of ret 51 was rather restricted. Our data indicated that ret isoforms are temporally and spatially regulated in mouse embryos and adult mouse. © 2003 Elsevier Science B.V. All rights reserved.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationBiochimica Et Biophysica Acta - Gene Structure And Expression, 2003, v. 1627 n. 1, p. 26-38 [How to Cite?]
DOI: http://dx.doi.org/10.1016/S0167-4781(03)00068-X
 
dc.identifier.doihttp://dx.doi.org/10.1016/S0167-4781(03)00068-X
 
dc.identifier.epage38
 
dc.identifier.hkuros88339
 
dc.identifier.isiWOS:000183223600004
 
dc.identifier.issn0167-4781
 
dc.identifier.issue1
 
dc.identifier.openurl
 
dc.identifier.pmid12759189
 
dc.identifier.scopuseid_2-s2.0-0038004062
 
dc.identifier.spage26
 
dc.identifier.urihttp://hdl.handle.net/10722/68251
 
dc.identifier.volume1627
 
dc.languageeng
 
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbaexp
 
dc.publisher.placeNetherlands
 
dc.relation.ispartofBiochimica et Biophysica Acta - Gene Structure and Expression
 
dc.relation.referencesReferences in Scopus
 
dc.rightsB B A - Gene Structure and Expression. Copyright © Elsevier BV.
 
dc.subject.meshAlternative Splicing
 
dc.subject.meshAmino Acid Sequence
 
dc.subject.meshAnimals
 
dc.subject.meshBase Sequence
 
dc.subject.meshIn Situ Hybridization
 
dc.subject.meshMice
 
dc.subject.meshMolecular Sequence Data
 
dc.subject.meshOrgan Specificity
 
dc.subject.meshProtein Isoforms - genetics
 
dc.subject.meshProto-Oncogene Proteins - genetics - metabolism
 
dc.subject.meshProto-Oncogene Proteins c-ret
 
dc.subject.meshRNA, Messenger - metabolism
 
dc.subject.meshReceptor Protein-Tyrosine Kinases - genetics - metabolism
 
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction
 
dc.subject3′ Splicing variants
 
dc.subjectDifferential expression
 
dc.subjectEmbryogenesis
 
dc.subjectRet protooncogene
 
dc.title3′ Splicing variants of ret receptor tyrosine kinase are differentially expressed in mouse embryos and in adult mice
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. University of Virginia Medical School