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Article: VCY2 protein interacts with the HECT domain of ubiquitin-protein ligase E3A

TitleVCY2 protein interacts with the HECT domain of ubiquitin-protein ligase E3A
Authors
KeywordsTestis
UBE3A
Ubiquitin-protein ligase
Ubiquitination
VCY2
Yeast two-hybrid
Issue Date2002
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2002, v. 296 n. 5, p. 1104-1111 How to Cite?
AbstractVCY2 locates in the AZFc region on chromosome Yq and is frequently deleted in infertile men with severe oligozoospermia or azoospermia. VCY2 is a testis-specific protein with unknown function. This study was to identify the protein that interacts with VCY2. We used the full-length VCY2 as bait to screen the human testis cDNA library using yeast two-hybrid approach. We identified a number of positive-interacting clones that encode ubiquitin-protein ligase E3A (UBE3A). UBE3A contains a HECT domain that binds VCY2. The specificity of the interaction was confirmed by co-immunoprecipitation and yeast mating. Northern blot analyses revealed two UBE3A transcripts 1.4 and 2 kb that were abundantly expressed in human testis. We also showed that both VCY2 and UBE3A mRNAs were expressed in ejaculated human spermatozoa, indicating that both genes localize in the germ cell compartment. These data suggest that UBE3A ubiquitination may be required for VCY2 function. © 2002 Elsevier Science (USA). All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/68242
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, EYMen_HK
dc.contributor.authorTse, JYMen_HK
dc.contributor.authorYao, KMen_HK
dc.contributor.authorTam, PCen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2010-09-06T06:02:43Z-
dc.date.available2010-09-06T06:02:43Z-
dc.date.issued2002en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 2002, v. 296 n. 5, p. 1104-1111en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/68242-
dc.description.abstractVCY2 locates in the AZFc region on chromosome Yq and is frequently deleted in infertile men with severe oligozoospermia or azoospermia. VCY2 is a testis-specific protein with unknown function. This study was to identify the protein that interacts with VCY2. We used the full-length VCY2 as bait to screen the human testis cDNA library using yeast two-hybrid approach. We identified a number of positive-interacting clones that encode ubiquitin-protein ligase E3A (UBE3A). UBE3A contains a HECT domain that binds VCY2. The specificity of the interaction was confirmed by co-immunoprecipitation and yeast mating. Northern blot analyses revealed two UBE3A transcripts 1.4 and 2 kb that were abundantly expressed in human testis. We also showed that both VCY2 and UBE3A mRNAs were expressed in ejaculated human spermatozoa, indicating that both genes localize in the germ cell compartment. These data suggest that UBE3A ubiquitination may be required for VCY2 function. © 2002 Elsevier Science (USA). All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.subjectTestisen_HK
dc.subjectUBE3Aen_HK
dc.subjectUbiquitin-protein ligaseen_HK
dc.subjectUbiquitinationen_HK
dc.subjectVCY2en_HK
dc.subjectYeast two-hybriden_HK
dc.subject.meshAmino Acid Sequenceen_HK
dc.subject.meshHumansen_HK
dc.subject.meshInfertility, Male - geneticsen_HK
dc.subject.meshLigases - chemistry - genetics - metabolismen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshPrecipitin Testsen_HK
dc.subject.meshProtein Structure, Tertiaryen_HK
dc.subject.meshProteins - genetics - metabolism - physiologyen_HK
dc.subject.meshRNA, Messenger - biosynthesisen_HK
dc.subject.meshSaccharomyces cerevisiae - genetics - growth & developmenten_HK
dc.subject.meshSequence Analysis, DNAen_HK
dc.subject.meshSequence Homology, Amino Aciden_HK
dc.subject.meshSpermatozoa - metabolismen_HK
dc.subject.meshTestis - metabolismen_HK
dc.subject.meshTissue Distributionen_HK
dc.subject.meshTwo-Hybrid System Techniquesen_HK
dc.subject.meshUbiquitin-Protein Ligasesen_HK
dc.subject.meshUbiquitins - metabolismen_HK
dc.titleVCY2 protein interacts with the HECT domain of ubiquitin-protein ligase E3Aen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-291X&volume=296&issue=5&spage=1104&epage=1111&date=2002&atitle=VCY2+protein+interacts+with+the+HECT+domain+of+ubiquitin-protein+ligase+E3Aen_HK
dc.identifier.emailWong, EYM: elainewg@hku.hken_HK
dc.identifier.emailYao, KM: kmyao@hku.hken_HK
dc.identifier.emailYeung, WSB: wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityWong, EYM=rp01718en_HK
dc.identifier.authorityYao, KM=rp00344en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0006-291X(02)02040-5en_HK
dc.identifier.pmid12207887-
dc.identifier.scopuseid_2-s2.0-0036386064en_HK
dc.identifier.hkuros74934en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036386064&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume296en_HK
dc.identifier.issue5en_HK
dc.identifier.spage1104en_HK
dc.identifier.epage1111en_HK
dc.identifier.isiWOS:000177987600013-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWong, EYM=36719382700en_HK
dc.identifier.scopusauthoridTse, JYM=7102607314en_HK
dc.identifier.scopusauthoridYao, KM=7403234578en_HK
dc.identifier.scopusauthoridTam, PC=7202539419en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK

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