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Article: Direct interaction of microtubule- and actin-based transport motors

TitleDirect interaction of microtubule- and actin-based transport motors
Authors
Issue Date1999
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/nature
Citation
Nature, 1999, v. 397 n. 6716, p. 267-270 How to Cite?
AbstractThe microtubule network is thought to be used for long-range transport of cellular components in animal cells whereas the actin network is proposed to be used for short-range transport, although the mechanism(s) by which this transport is coordinated is poorly understood. For example, in sea urchins long-range Ca2+ -regulated transport of exocytotic vesicles requires a microtubule-based motor, whereas an actin-based motor is used for short- range transport. In neurons, microtubule-based kinesin motor proteins are used for long-range vesicular transport but microtubules do not extend into the neuronal termini, where actin filaments form the cytoskeletal framework, and kinesins are rapidly degraded upon their arrival in neuronal termini, indicating that vesicles may have to be transferred from microtubules to actin tracks to reach their-final destination. Here we show that an actin- based vesicle-transport motor, MyoVA (ref. 6), can interact directly with a microtubule-based transport motor, KhcU. As would be expected if these complexes were functional, they also contain kinesin light chains and the localization of MyoVA and KhcU overlaps in the cell. These results indicate that cellular transport is, in part, coordinated through the direct interaction of different motor molecules.
Persistent Identifierhttp://hdl.handle.net/10722/68236
ISSN
2015 Impact Factor: 38.138
2015 SCImago Journal Rankings: 21.936
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHuang, JDen_HK
dc.contributor.authorBrady, STen_HK
dc.contributor.authorRichards, BWen_HK
dc.contributor.authorStenoien, Den_HK
dc.contributor.authorResau, JHen_HK
dc.contributor.authorCopeland, NGen_HK
dc.contributor.authorJenkins, NAen_HK
dc.date.accessioned2010-09-06T06:02:39Z-
dc.date.available2010-09-06T06:02:39Z-
dc.date.issued1999en_HK
dc.identifier.citationNature, 1999, v. 397 n. 6716, p. 267-270en_HK
dc.identifier.issn0028-0836en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68236-
dc.description.abstractThe microtubule network is thought to be used for long-range transport of cellular components in animal cells whereas the actin network is proposed to be used for short-range transport, although the mechanism(s) by which this transport is coordinated is poorly understood. For example, in sea urchins long-range Ca2+ -regulated transport of exocytotic vesicles requires a microtubule-based motor, whereas an actin-based motor is used for short- range transport. In neurons, microtubule-based kinesin motor proteins are used for long-range vesicular transport but microtubules do not extend into the neuronal termini, where actin filaments form the cytoskeletal framework, and kinesins are rapidly degraded upon their arrival in neuronal termini, indicating that vesicles may have to be transferred from microtubules to actin tracks to reach their-final destination. Here we show that an actin- based vesicle-transport motor, MyoVA (ref. 6), can interact directly with a microtubule-based transport motor, KhcU. As would be expected if these complexes were functional, they also contain kinesin light chains and the localization of MyoVA and KhcU overlaps in the cell. These results indicate that cellular transport is, in part, coordinated through the direct interaction of different motor molecules.en_HK
dc.languageengen_HK
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/natureen_HK
dc.relation.ispartofNatureen_HK
dc.subject.meshActins - genetics - physiologyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBiological Transporten_HK
dc.subject.meshBrain - metabolismen_HK
dc.subject.meshCell Lineen_HK
dc.subject.meshEscherichia colien_HK
dc.subject.meshFluorescent Antibody Techniqueen_HK
dc.subject.meshIntermediate Filament Proteins - genetics - physiologyen_HK
dc.subject.meshKinesin - genetics - physiologyen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMicrotubules - physiologyen_HK
dc.subject.meshMolecular Motor Proteins - physiologyen_HK
dc.subject.meshMyosin Heavy Chainsen_HK
dc.subject.meshMyosin Type Ven_HK
dc.subject.meshPrecipitin Testsen_HK
dc.subject.meshProtein Bindingen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRecombinant Fusion Proteins - genetics - metabolismen_HK
dc.subject.meshSaccharomyces cerevisiae - geneticsen_HK
dc.titleDirect interaction of microtubule- and actin-based transport motorsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0028-0836&volume=397&spage=267&epage=270&date=1999&atitle=Direct+interaction+of+microtubule-+and+actin-based+transport+motorsen_HK
dc.identifier.emailHuang, JD:jdhuang@hkucc.hku.hken_HK
dc.identifier.authorityHuang, JD=rp00451en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1038/16722en_HK
dc.identifier.pmid9930703en_HK
dc.identifier.scopuseid_2-s2.0-0033590558en_HK
dc.identifier.hkuros43030en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033590558&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume397en_HK
dc.identifier.issue6716en_HK
dc.identifier.spage267en_HK
dc.identifier.epage270en_HK
dc.identifier.isiWOS:000078184800056-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridHuang, JD=8108660600en_HK
dc.identifier.scopusauthoridBrady, ST=16214595400en_HK
dc.identifier.scopusauthoridRichards, BW=7202100377en_HK
dc.identifier.scopusauthoridStenoien, D=6603375764en_HK
dc.identifier.scopusauthoridResau, JH=16158717400en_HK
dc.identifier.scopusauthoridCopeland, NG=35374759300en_HK
dc.identifier.scopusauthoridJenkins, NA=35379887700en_HK

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