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Article: Transcriptional regulation of transferrin receptor expression during phorbol-ester-induced HL-60 cell differentiation. Evidence for a negative regulatory role of the phorbol-ester-responsive element-like sequence

TitleTranscriptional regulation of transferrin receptor expression during phorbol-ester-induced HL-60 cell differentiation. Evidence for a negative regulatory role of the phorbol-ester-responsive element-like sequence
Authors
Keywords4β-phorbol 12-myristate 13-acetate
HL-60 cells
phorbol-ester-responsive element
transcriptional regulation
transferrin receptor
Issue Date1996
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/EJB
Citation
European Journal Of Biochemistry, 1996, v. 236 n. 2, p. 614-619 How to Cite?
AbstractThe mechanism involved in the regulation of transferrin receptor (TfR) expression during phorbol-ester-induced HL-60 cell differentiation was investigated. The mRNA of the TfR was constitutively expressed in proliferating HL-60 cells. Treatment of the cells with phorbol 12-myristate 13-acetate (PMA) for 24 h resulted in a gradual decrease in the expression of the TfR mRNA. Nuclear run-on assays revealed that the transcription of the TfR gene was inhibited by prior treatment of cells with PMA. The effect of PMA on the binding of nuclear proteins to the TfR gene promoter region was then investigated. Based on sequence similarity and previous footprinting data, the promoter region of the TfR gene seems to contain a sequence like that of the phorbol-ester-responsive element (TRE). Our results showed that the binding of nuclear extracts to the TfR gene promoter region containing the TRE-like sequence was increased in PMA-treated cells. This binding activity could be abolished by prior incubation of the nuclear extracts with a synthetic oligonucleotide containing the consensus TRE sequence. In vitro transcription assays revealed that prior incubation of the nuclear extracts of PMA-treated cells with excess consensus TRE oligonucleotide enhanced the gene transcription driven by the TfR gene promoter. These findings suggest that the TRE-like element may play a role in the inhibition of TfR gene transcription.
Persistent Identifierhttp://hdl.handle.net/10722/68218
ISSN
2006 Impact Factor: 3.579
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLok, CNen_HK
dc.contributor.authorChan, KFJen_HK
dc.contributor.authorLoh, TTen_HK
dc.date.accessioned2010-09-06T06:02:29Z-
dc.date.available2010-09-06T06:02:29Z-
dc.date.issued1996en_HK
dc.identifier.citationEuropean Journal Of Biochemistry, 1996, v. 236 n. 2, p. 614-619en_HK
dc.identifier.issn0014-2956en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68218-
dc.description.abstractThe mechanism involved in the regulation of transferrin receptor (TfR) expression during phorbol-ester-induced HL-60 cell differentiation was investigated. The mRNA of the TfR was constitutively expressed in proliferating HL-60 cells. Treatment of the cells with phorbol 12-myristate 13-acetate (PMA) for 24 h resulted in a gradual decrease in the expression of the TfR mRNA. Nuclear run-on assays revealed that the transcription of the TfR gene was inhibited by prior treatment of cells with PMA. The effect of PMA on the binding of nuclear proteins to the TfR gene promoter region was then investigated. Based on sequence similarity and previous footprinting data, the promoter region of the TfR gene seems to contain a sequence like that of the phorbol-ester-responsive element (TRE). Our results showed that the binding of nuclear extracts to the TfR gene promoter region containing the TRE-like sequence was increased in PMA-treated cells. This binding activity could be abolished by prior incubation of the nuclear extracts with a synthetic oligonucleotide containing the consensus TRE sequence. In vitro transcription assays revealed that prior incubation of the nuclear extracts of PMA-treated cells with excess consensus TRE oligonucleotide enhanced the gene transcription driven by the TfR gene promoter. These findings suggest that the TRE-like element may play a role in the inhibition of TfR gene transcription.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/EJBen_HK
dc.relation.ispartofEuropean Journal of Biochemistryen_HK
dc.rightsEuropean Journal of Biochemistry. Copyright © Blackwell Publishing Ltd.en_HK
dc.subject4β-phorbol 12-myristate 13-acetateen_HK
dc.subjectHL-60 cellsen_HK
dc.subjectphorbol-ester-responsive elementen_HK
dc.subjecttranscriptional regulationen_HK
dc.subjecttransferrin receptoren_HK
dc.titleTranscriptional regulation of transferrin receptor expression during phorbol-ester-induced HL-60 cell differentiation. Evidence for a negative regulatory role of the phorbol-ester-responsive element-like sequenceen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0014-2956&volume=236&spage=614&epage=619&date=1996&atitle=Transcriptional+regulation+of+transferrin+receptor+expression+during+phorbol-ester-induced+HL-60+cell+differentiationen_HK
dc.identifier.emailLok, CN: cnlok@hku.hken_HK
dc.identifier.emailChan, KFJ: kfjchan@hkucc.hku.hken_HK
dc.identifier.authorityLok, CN=rp00752en_HK
dc.identifier.authorityChan, KFJ=rp00378en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1111/j.1432-1033.1996.00614.x-
dc.identifier.pmid8612636-
dc.identifier.scopuseid_2-s2.0-0029877060en_HK
dc.identifier.hkuros11533en_HK
dc.identifier.volume236en_HK
dc.identifier.issue2en_HK
dc.identifier.spage614en_HK
dc.identifier.epage619en_HK
dc.identifier.isiWOS:A1996TY99000036-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLok, CN=7006410829en_HK
dc.identifier.scopusauthoridChan, KFJ=7406035590en_HK
dc.identifier.scopusauthoridLoh, TT=36849142100en_HK

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