File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Osteogenic behavior of alginate encapsulated bone marrow stromal cells: An in vitro study

TitleOsteogenic behavior of alginate encapsulated bone marrow stromal cells: An in vitro study
Authors
Issue Date2008
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0957-4530
Citation
Journal Of Materials Science: Materials In Medicine, 2008, v. 19 n. 5, p. 2113-2119 How to Cite?
AbstractSodium alginate is a useful polymer for the encapsulation and immobilization of a variety of cells in tissue engineering because it is biocompatible, biodegradable and easy to process into injectable microbeads. Despite these properties, little is known of the efficacy of calcium cross-linked alginate gel beads as a biodegradable scaffold for osteogenic cell proliferation and differentiation. In this study, we investigated the ability of rabbit derived bone marrow cells (BMCs) to proliferate and differentiate in alginate microbeads and compared them with BMCs cultured in poly-l-lysine (PLL) coated microbeads and on conventional 2D plastic surfaces. Results show that levels of proliferation and differentiation in microbeads and on tissue culture plastics were comparable. Cell proliferation in microbeads however diminished after fortification with a coating layer of PLL. Maximum cell numbers observed were, 3.32 × 105 ± 1.72 × 103; 3.11 × 105 ± 1.52 × 103 and 3.28 × 10 5 ± 1.21 × 103 for the uncoated, PLL coated and plastic surface groups respectively. Alkaline phosphatase and protein expressions reflected the stage of cell differentiation. We conclude that calcium cross-linked alginate microbeads can act as a scaffold for BMC proliferation and osteogenic differentiation and has potential for use as 3D degradable scaffold. © 2007 Springer Science+Business Media, LLC.
Persistent Identifierhttp://hdl.handle.net/10722/68206
ISSN
2015 Impact Factor: 2.272
2013 SCImago Journal Rankings: 0.897
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorAbbah, SAen_HK
dc.contributor.authorLu, WWen_HK
dc.contributor.authorChan, Den_HK
dc.contributor.authorCheung, KMCen_HK
dc.contributor.authorLiu, WGen_HK
dc.contributor.authorZhao, Fen_HK
dc.contributor.authorLi, ZYen_HK
dc.contributor.authorLeong, JCYen_HK
dc.contributor.authorLuk, KDKen_HK
dc.date.accessioned2010-09-06T06:02:22Z-
dc.date.available2010-09-06T06:02:22Z-
dc.date.issued2008en_HK
dc.identifier.citationJournal Of Materials Science: Materials In Medicine, 2008, v. 19 n. 5, p. 2113-2119en_HK
dc.identifier.issn0957-4530en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68206-
dc.description.abstractSodium alginate is a useful polymer for the encapsulation and immobilization of a variety of cells in tissue engineering because it is biocompatible, biodegradable and easy to process into injectable microbeads. Despite these properties, little is known of the efficacy of calcium cross-linked alginate gel beads as a biodegradable scaffold for osteogenic cell proliferation and differentiation. In this study, we investigated the ability of rabbit derived bone marrow cells (BMCs) to proliferate and differentiate in alginate microbeads and compared them with BMCs cultured in poly-l-lysine (PLL) coated microbeads and on conventional 2D plastic surfaces. Results show that levels of proliferation and differentiation in microbeads and on tissue culture plastics were comparable. Cell proliferation in microbeads however diminished after fortification with a coating layer of PLL. Maximum cell numbers observed were, 3.32 × 105 ± 1.72 × 103; 3.11 × 105 ± 1.52 × 103 and 3.28 × 10 5 ± 1.21 × 103 for the uncoated, PLL coated and plastic surface groups respectively. Alkaline phosphatase and protein expressions reflected the stage of cell differentiation. We conclude that calcium cross-linked alginate microbeads can act as a scaffold for BMC proliferation and osteogenic differentiation and has potential for use as 3D degradable scaffold. © 2007 Springer Science+Business Media, LLC.en_HK
dc.languageengen_HK
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0957-4530en_HK
dc.relation.ispartofJournal of Materials Science: Materials in Medicineen_HK
dc.subject.meshAlginates - chemistryen_HK
dc.subject.meshAlkaline Phosphatase - metabolismen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBiocompatible Materials - chemistryen_HK
dc.subject.meshBone Marrow Cells - cytologyen_HK
dc.subject.meshCalcium - chemistryen_HK
dc.subject.meshCell Differentiationen_HK
dc.subject.meshCell Proliferationen_HK
dc.subject.meshCell Survivalen_HK
dc.subject.meshCulture Media, Serum-Free - chemistryen_HK
dc.subject.meshGlucuronic Acid - chemistryen_HK
dc.subject.meshHexuronic Acids - chemistryen_HK
dc.subject.meshOsteogenesisen_HK
dc.subject.meshPlasticsen_HK
dc.subject.meshPolylysine - chemistryen_HK
dc.subject.meshRabbitsen_HK
dc.subject.meshTissue Engineering - methodsen_HK
dc.titleOsteogenic behavior of alginate encapsulated bone marrow stromal cells: An in vitro studyen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0957-4530&volume=19&issue=5&spage=2113&epage=9&date=2008&atitle=Osteogenic+behavior+of+alginate+encapsulated+bone+marrow+stromal+cells:+an+in+vitro+studyen_HK
dc.identifier.emailLu, WW:wwlu@hku.hken_HK
dc.identifier.emailChan, D:chand@hkucc.hku.hken_HK
dc.identifier.emailCheung, KMC:cheungmc@hku.hken_HK
dc.identifier.emailLuk, KDK:hcm21000@hku.hken_HK
dc.identifier.authorityLu, WW=rp00411en_HK
dc.identifier.authorityChan, D=rp00540en_HK
dc.identifier.authorityCheung, KMC=rp00387en_HK
dc.identifier.authorityLuk, KDK=rp00333en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s10856-006-0013-1en_HK
dc.identifier.pmid17136608en_HK
dc.identifier.scopuseid_2-s2.0-41149166123en_HK
dc.identifier.hkuros144254en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-41149166123&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume19en_HK
dc.identifier.issue5en_HK
dc.identifier.spage2113en_HK
dc.identifier.epage2119en_HK
dc.identifier.isiWOS:000254202300031-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridAbbah, SA=14032930600en_HK
dc.identifier.scopusauthoridLu, WW=7404215221en_HK
dc.identifier.scopusauthoridChan, D=7402216545en_HK
dc.identifier.scopusauthoridCheung, KMC=7402406754en_HK
dc.identifier.scopusauthoridLiu, WG=35269689700en_HK
dc.identifier.scopusauthoridZhao, F=36040646100en_HK
dc.identifier.scopusauthoridLi, ZY=35784563200en_HK
dc.identifier.scopusauthoridLeong, JCY=35560782200en_HK
dc.identifier.scopusauthoridLuk, KDK=7201921573en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats