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Article: Expression of chondroitin sulfate proteoglycans in the chiasm of mouse embryos

TitleExpression of chondroitin sulfate proteoglycans in the chiasm of mouse embryos
Authors
Issue Date2000
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31248
Citation
Journal Of Comparative Neurology, 2000, v. 417 n. 2, p. 153-163 How to Cite?
AbstractChondroitin sulfate (CS) proteoglycans have been implicated as molecules that are involved in axon guidance in the developing neural pathways. The spatiotemporal expression of CS was investigated in the developing retinofugal pathway in mouse embryos by using the CS-56 antibody. Immunoreactive CS was detected in inner regions of the retina as early as embryonic day 11 (E11). Its expression in subsequent stages of development followed a centrifugal, receding gradient that appeared to correlate with the sequence of axogenesis in the retina. In the chiasm, immunoreactive CS was expressed at E12, before the arrival of retinal axons. When the retinal axons navigated in the chiasm at E13-E14, immunoreactive CS remained at a low level in the optic fiber layer of the chiasm but was observed prominently in the caudal parts of the ventral diencephalon. This pattern followed closely the array of stage-specific-embryonic-antigen-1-positive neurons in the ventral diencephalon, with a V-shaped configuration that bordered the posterior boundary of the retinal axons, and a rostral raphe extension that ran across the decussating axons in the chiasm. Thus, the CS epitope is implicated in patterning the course of early retinal axons and in regulating axon divergence in the chiasm. At the lateral region of the chiasm, where the retinal axons cross the midline and approach the optic tract, a CS- immunopositive region coincided with the region in which active sorting of dorsal retinal axons from ventral retinal axons occurs. Moreover, at the threshold of the optic tract, the immunoreactive CS was restricted only to the deep part of the optic fiber layer, suggesting an inhibitory role of the CS epitope in repelling newly arrived axons to superficial regions of the optic tract during the development of chronotopic order at this part of the retinofugal pathway.
Persistent Identifierhttp://hdl.handle.net/10722/68194
ISSN
2015 Impact Factor: 3.331
2015 SCImago Journal Rankings: 2.345
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChung, KYen_HK
dc.contributor.authorShum, DKYen_HK
dc.contributor.authorChan, SOen_HK
dc.date.accessioned2010-09-06T06:02:15Z-
dc.date.available2010-09-06T06:02:15Z-
dc.date.issued2000en_HK
dc.identifier.citationJournal Of Comparative Neurology, 2000, v. 417 n. 2, p. 153-163en_HK
dc.identifier.issn0021-9967en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68194-
dc.description.abstractChondroitin sulfate (CS) proteoglycans have been implicated as molecules that are involved in axon guidance in the developing neural pathways. The spatiotemporal expression of CS was investigated in the developing retinofugal pathway in mouse embryos by using the CS-56 antibody. Immunoreactive CS was detected in inner regions of the retina as early as embryonic day 11 (E11). Its expression in subsequent stages of development followed a centrifugal, receding gradient that appeared to correlate with the sequence of axogenesis in the retina. In the chiasm, immunoreactive CS was expressed at E12, before the arrival of retinal axons. When the retinal axons navigated in the chiasm at E13-E14, immunoreactive CS remained at a low level in the optic fiber layer of the chiasm but was observed prominently in the caudal parts of the ventral diencephalon. This pattern followed closely the array of stage-specific-embryonic-antigen-1-positive neurons in the ventral diencephalon, with a V-shaped configuration that bordered the posterior boundary of the retinal axons, and a rostral raphe extension that ran across the decussating axons in the chiasm. Thus, the CS epitope is implicated in patterning the course of early retinal axons and in regulating axon divergence in the chiasm. At the lateral region of the chiasm, where the retinal axons cross the midline and approach the optic tract, a CS- immunopositive region coincided with the region in which active sorting of dorsal retinal axons from ventral retinal axons occurs. Moreover, at the threshold of the optic tract, the immunoreactive CS was restricted only to the deep part of the optic fiber layer, suggesting an inhibitory role of the CS epitope in repelling newly arrived axons to superficial regions of the optic tract during the development of chronotopic order at this part of the retinofugal pathway.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31248en_HK
dc.relation.ispartofJournal of Comparative Neurologyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshChondroitin Sulfate Proteoglycans - metabolismen_HK
dc.subject.meshDiencephalon - embryologyen_HK
dc.subject.meshEmbryo, Mammalian - metabolism - physiologyen_HK
dc.subject.meshEmbryonic and Fetal Developmenten_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshMice - embryologyen_HK
dc.subject.meshMicroscopy, Confocalen_HK
dc.subject.meshOptic Chiasm - embryologyen_HK
dc.subject.meshRetina - embryologyen_HK
dc.titleExpression of chondroitin sulfate proteoglycans in the chiasm of mouse embryosen_HK
dc.typeArticleen_HK
dc.identifier.emailShum, DKY:shumdkhk@hkucc.hku.hken_HK
dc.identifier.authorityShum, DKY=rp00321en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/(SICI)1096-9861(20000207)417:2<153::AID-CNE2>3.0.CO;2-Den_HK
dc.identifier.pmid10660894-
dc.identifier.scopuseid_2-s2.0-0034615039en_HK
dc.identifier.hkuros53317en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034615039&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume417en_HK
dc.identifier.issue2en_HK
dc.identifier.spage153en_HK
dc.identifier.epage163en_HK
dc.identifier.isiWOS:000084633500002-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChung, KY=7404085795en_HK
dc.identifier.scopusauthoridShum, DKY=7004824447en_HK
dc.identifier.scopusauthoridChan, SO=7404256181en_HK

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