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Article: Single-stranded oligonucleotide-mediated gene repair in mammalian cells has a mechanism distinct from homologous recombination repair

TitleSingle-stranded oligonucleotide-mediated gene repair in mammalian cells has a mechanism distinct from homologous recombination repair
Authors
Issue Date2006
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2006, v. 350 n. 3, p. 568-573 How to Cite?
AbstractSingle-stranded DNA oligonucleotide (SSO)-mediated gene repair has great potentials for gene therapy and functional genomic studies. However, its underlying mechanism remains unclear. Previous studies from other groups have suggested that DNA damage response via the ATM/ATR pathway may be involved in this process. In this study, we measured the effect of two ATM/ATR inhibitors caffeine and pentoxifylline on the correction efficiency in SSO-mediated gene repair. We also checked their effect on double-stranded break (DSB)-induced homologous recombination repair (HRR) as a control, which is well known to be dependent on the ATM/ATR pathway. We found these inhibitors could completely inhibit DSB-induced HRR, but could only partially inhibit SSO-mediated process, indicating SSO-mediated gene repair is not dependent on the ATM/ATR pathway. Furthermore, we found that thymidine treatment promotes SSO-mediated gene repair, but inhibits DSB-induced HRR. Collectively, our results demonstrate that SSO-mediated and DSB-induced gene repairs have distinct mechanisms. © 2006 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/68186
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWang, Zen_HK
dc.contributor.authorZhou, ZJen_HK
dc.contributor.authorLiu, DPen_HK
dc.contributor.authorHuang, JDen_HK
dc.date.accessioned2010-09-06T06:02:10Z-
dc.date.available2010-09-06T06:02:10Z-
dc.date.issued2006en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 2006, v. 350 n. 3, p. 568-573en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/68186-
dc.description.abstractSingle-stranded DNA oligonucleotide (SSO)-mediated gene repair has great potentials for gene therapy and functional genomic studies. However, its underlying mechanism remains unclear. Previous studies from other groups have suggested that DNA damage response via the ATM/ATR pathway may be involved in this process. In this study, we measured the effect of two ATM/ATR inhibitors caffeine and pentoxifylline on the correction efficiency in SSO-mediated gene repair. We also checked their effect on double-stranded break (DSB)-induced homologous recombination repair (HRR) as a control, which is well known to be dependent on the ATM/ATR pathway. We found these inhibitors could completely inhibit DSB-induced HRR, but could only partially inhibit SSO-mediated process, indicating SSO-mediated gene repair is not dependent on the ATM/ATR pathway. Furthermore, we found that thymidine treatment promotes SSO-mediated gene repair, but inhibits DSB-induced HRR. Collectively, our results demonstrate that SSO-mediated and DSB-induced gene repairs have distinct mechanisms. © 2006 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.subject.meshCell Lineen_HK
dc.subject.meshDNA Breaks, Single-Strandeden_HK
dc.subject.meshDNA Repair - physiologyen_HK
dc.subject.meshDNA, Single-Stranded - metabolismen_HK
dc.subject.meshDNA-Binding Proteins - metabolismen_HK
dc.subject.meshHeLa Cellsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshKidney - physiologyen_HK
dc.subject.meshRecombination, Genetic - physiologyen_HK
dc.titleSingle-stranded oligonucleotide-mediated gene repair in mammalian cells has a mechanism distinct from homologous recombination repairen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-291X&volume=350 &issue=3&spage=568&epage=573&date=2006&atitle=Single-Stranded+Oligonucleotide-mediated+gene+repair+in+mammalian+cells+has+a+mechanism+distinct+from+homologous+recombination+repair.+en_HK
dc.identifier.emailZhou, ZJ:zhongjun@hkucc.hku.hken_HK
dc.identifier.emailHuang, JD:jdhuang@hkucc.hku.hken_HK
dc.identifier.authorityZhou, ZJ=rp00503en_HK
dc.identifier.authorityHuang, JD=rp00451en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.bbrc.2006.09.078en_HK
dc.identifier.pmid17026965-
dc.identifier.scopuseid_2-s2.0-33749658767en_HK
dc.identifier.hkuros127230en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33749658767&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume350en_HK
dc.identifier.issue3en_HK
dc.identifier.spage568en_HK
dc.identifier.epage573en_HK
dc.identifier.isiWOS:000241584300011-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWang, Z=40162656400en_HK
dc.identifier.scopusauthoridZhou, ZJ=8631856300en_HK
dc.identifier.scopusauthoridLiu, DP=21934191400en_HK
dc.identifier.scopusauthoridHuang, JD=8108660600en_HK

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