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Article: CDX-1 and CDX-2 are expressed in human colonic mucosa and are down-regulated in patients with Hirschsprung's disease associated enterocolitis

TitleCDX-1 and CDX-2 are expressed in human colonic mucosa and are down-regulated in patients with Hirschsprung's disease associated enterocolitis
Authors
KeywordsCaudal type homeobox gene
Human colon
Issue Date2001
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbadis
Citation
Biochimica Et Biophysica Acta - Molecular Basis Of Disease, 2001, v. 1537 n. 2, p. 89-100 How to Cite?
AbstractCaudal type homeobox gene-1 and -2 (CDX-1 and CDX-2), homologues of the Drosophila homeobox gene caudal, encode transcription factors in endoderm derived tissues of the intestine. CDX genes control proliferation and differentiation of intestinal mucosal cells and colon cancer cells. Hirschsprung's Disease (HD) or congenital intestinal aganglionosis, a major developmental anomaly of intestine, which causes functional intestinal obstruction, is frequently associated with enterocolitis. Aetiology of HD-associated enterocolitis (HDEC) remains obscure. Reduction of gut mucosal enteroendocrine cells, and inefficient transfer of the secretory immunoglobulin A across the gut mucosal cell were shown to be associated with enterocolitis in HD patients suggesting that mucosa may directly involve in the pathophysiology of HDEC. This study aims to ascertain whether the CDX-1 and CDX-2 genes, that control the proliferation and differentiation of mucosal cells, play a role in HDEC. Using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridisation, we analysed the expression of CDX-1 and CDX-2 genes in colon specimens of normal controls, necrotising enterocolitis (NEC) infants, and HD patients with and without enterocolitis. We showed for the first time that CDX-1 and CDX-2 genes were expressed in the colonic mucosal epithelium in normal, NEC and in HD infants. However, the expressions of both genes were reduced in patients with HDEC. Our findings suggest that reduced expression of CDX-1 and CDX-2 genes in mucosa may be associated with the development of HDEC. © 2001 Elsevier Science B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/68166
ISSN
2015 Impact Factor: 5.158
2015 SCImago Journal Rankings: 2.718
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLui, VCHen_HK
dc.contributor.authorLi, Len_HK
dc.contributor.authorSham, MHen_HK
dc.contributor.authorTam, PKHen_HK
dc.date.accessioned2010-09-06T06:01:59Z-
dc.date.available2010-09-06T06:01:59Z-
dc.date.issued2001en_HK
dc.identifier.citationBiochimica Et Biophysica Acta - Molecular Basis Of Disease, 2001, v. 1537 n. 2, p. 89-100en_HK
dc.identifier.issn0925-4439en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68166-
dc.description.abstractCaudal type homeobox gene-1 and -2 (CDX-1 and CDX-2), homologues of the Drosophila homeobox gene caudal, encode transcription factors in endoderm derived tissues of the intestine. CDX genes control proliferation and differentiation of intestinal mucosal cells and colon cancer cells. Hirschsprung's Disease (HD) or congenital intestinal aganglionosis, a major developmental anomaly of intestine, which causes functional intestinal obstruction, is frequently associated with enterocolitis. Aetiology of HD-associated enterocolitis (HDEC) remains obscure. Reduction of gut mucosal enteroendocrine cells, and inefficient transfer of the secretory immunoglobulin A across the gut mucosal cell were shown to be associated with enterocolitis in HD patients suggesting that mucosa may directly involve in the pathophysiology of HDEC. This study aims to ascertain whether the CDX-1 and CDX-2 genes, that control the proliferation and differentiation of mucosal cells, play a role in HDEC. Using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridisation, we analysed the expression of CDX-1 and CDX-2 genes in colon specimens of normal controls, necrotising enterocolitis (NEC) infants, and HD patients with and without enterocolitis. We showed for the first time that CDX-1 and CDX-2 genes were expressed in the colonic mucosal epithelium in normal, NEC and in HD infants. However, the expressions of both genes were reduced in patients with HDEC. Our findings suggest that reduced expression of CDX-1 and CDX-2 genes in mucosa may be associated with the development of HDEC. © 2001 Elsevier Science B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbadisen_HK
dc.relation.ispartofBiochimica et Biophysica Acta - Molecular Basis of Diseaseen_HK
dc.rightsBiochimica et Biophysica Acta. Copyright © Elsevier BV.en_HK
dc.subjectCaudal type homeobox geneen_HK
dc.subjectHuman colonen_HK
dc.subject.meshChild, Preschoolen_HK
dc.subject.meshCloning, Molecularen_HK
dc.subject.meshColon - metabolismen_HK
dc.subject.meshDown-Regulationen_HK
dc.subject.meshEnterocolitis - etiology - metabolismen_HK
dc.subject.meshEpithelium - metabolismen_HK
dc.subject.meshGene Expression Regulationen_HK
dc.subject.meshHirschsprung Disease - complications - metabolismen_HK
dc.subject.meshHomeodomain Proteins - genetics - metabolismen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshIn Situ Hybridizationen_HK
dc.subject.meshInfanten_HK
dc.subject.meshInfant, Newbornen_HK
dc.subject.meshIntestinal Mucosa - metabolismen_HK
dc.subject.meshRNA, Messenger - analysisen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshTrans-Activatorsen_HK
dc.titleCDX-1 and CDX-2 are expressed in human colonic mucosa and are down-regulated in patients with Hirschsprung's disease associated enterocolitisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0925-4439&volume=1537&issue=2&spage=89&epage=100&date=2001&atitle=CDX-1+and+CDX-2+are+expressed+in+human+colonic+mucosa+and+are+down-regulated+in+patients+with+Hirschsprung%27s+disease+associated+enterocolitisen_HK
dc.identifier.emailLui, VCH: vchlui@hkucc.hku.hken_HK
dc.identifier.emailSham, MH: mhsham@hkucc.hku.hken_HK
dc.identifier.emailTam, PKH: paultam@hkucc.hku.hken_HK
dc.identifier.authorityLui, VCH=rp00363en_HK
dc.identifier.authoritySham, MH=rp00380en_HK
dc.identifier.authorityTam, PKH=rp00060en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0925-4439(01)00056-4en_HK
dc.identifier.pmid11566252-
dc.identifier.scopuseid_2-s2.0-0035964966en_HK
dc.identifier.hkuros63602en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035964966&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1537en_HK
dc.identifier.issue2en_HK
dc.identifier.spage89en_HK
dc.identifier.epage100en_HK
dc.identifier.isiWOS:000171408700001-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLui, VCH=7004231344en_HK
dc.identifier.scopusauthoridLi, L=7501448457en_HK
dc.identifier.scopusauthoridSham, MH=7003729109en_HK
dc.identifier.scopusauthoridTam, PKH=7202539421en_HK

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