File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Decellularization of chondrocyte-encapsulated collagen microspheres: A three-dimensional model to study the effects of acellular matrix on stem cell fate

TitleDecellularization of chondrocyte-encapsulated collagen microspheres: A three-dimensional model to study the effects of acellular matrix on stem cell fate
Authors
Issue Date2009
PublisherMary Ann Liebert, Inc. Publishers. The Journal's web site is located at http://www.liebertpub.com/publication.aspx?pub_id=261
Citation
Tissue Engineering - Part C: Methods, 2009, v. 15 n. 4, p. 697-706 How to Cite?
AbstractExtracellular matrix (ECM) partially constitutes the stem cell niche. Reconstituting the ECM niche in a three-dimensional (3D) configuration will significantly enhance our understanding of how stem cells interact with and respond to the ECM niche. In this study, we aimed to reconstitute a glycosaminoglycan (GAG)-rich ECM using a microencapsulation technology, produce acellular matrix using a decellularization technique, and investigate the effect of acellular matrix on stem cell fate by repopulating the matrix with human mesenchymal stem cells (hMSCs). We demonstrated that porcine chondrocytes were able to deposit a GAG-rich ECM within the 3D collagen microsphere. All decellularization treatment groups resulted in significant removal of chondrocyte nuclei, but acellular matrix was only achieved using 2% sodium deoxycholate. Nevertheless, decellularization resulted in significant loss in GAG content in almost all treatment groups, and the 2% sodium deoxycholate group was able to preserve about 40% of the GAGs compared with the control group. We further demonstrated that hMSCs seeded onto the decellularized microspheres were able to survive and penetrate into the centre, while hMSCs seeded in the acellular matrix showed positive immunostaining against sox9, indicating that they may be differentiating toward the chondrogenic lineage without the need to supplement the chondrogenic differentiation medium. © 2009 Mary Ann Liebert, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/68157
ISSN
2015 SCImago Journal Rankings: 1.141
ISI Accession Number ID
Funding AgencyGrant Number
AOSpineAOSBRC-07-06
Innovation and Technology Commission
Hong Kong GovernmentGHP/050/06
University Research Committee
University of Hong Kong10206799
Strategic Research Theme on Development, Growth and Reproduction
Funding Information:

This work was supported by grants from AOSpine (AOSBRC-07-06); the Innovation and Technology Commission, the Hong Kong Government (GHP/050/06); the University Research Committee, the University of Hong Kong (10206799); and the Strategic Research Theme on Development, Growth and Reproduction. The authors thank Ms. Vicki Geall for editing the manuscript.

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorCheng, HWen_HK
dc.contributor.authorTsui, YKen_HK
dc.contributor.authorCheung, KMCen_HK
dc.contributor.authorChan, Den_HK
dc.contributor.authorChan, BPen_HK
dc.date.accessioned2010-09-06T06:01:54Z-
dc.date.available2010-09-06T06:01:54Z-
dc.date.issued2009en_HK
dc.identifier.citationTissue Engineering - Part C: Methods, 2009, v. 15 n. 4, p. 697-706en_HK
dc.identifier.issn1937-3384en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68157-
dc.description.abstractExtracellular matrix (ECM) partially constitutes the stem cell niche. Reconstituting the ECM niche in a three-dimensional (3D) configuration will significantly enhance our understanding of how stem cells interact with and respond to the ECM niche. In this study, we aimed to reconstitute a glycosaminoglycan (GAG)-rich ECM using a microencapsulation technology, produce acellular matrix using a decellularization technique, and investigate the effect of acellular matrix on stem cell fate by repopulating the matrix with human mesenchymal stem cells (hMSCs). We demonstrated that porcine chondrocytes were able to deposit a GAG-rich ECM within the 3D collagen microsphere. All decellularization treatment groups resulted in significant removal of chondrocyte nuclei, but acellular matrix was only achieved using 2% sodium deoxycholate. Nevertheless, decellularization resulted in significant loss in GAG content in almost all treatment groups, and the 2% sodium deoxycholate group was able to preserve about 40% of the GAGs compared with the control group. We further demonstrated that hMSCs seeded onto the decellularized microspheres were able to survive and penetrate into the centre, while hMSCs seeded in the acellular matrix showed positive immunostaining against sox9, indicating that they may be differentiating toward the chondrogenic lineage without the need to supplement the chondrogenic differentiation medium. © 2009 Mary Ann Liebert, Inc.en_HK
dc.languageengen_HK
dc.publisherMary Ann Liebert, Inc. Publishers. The Journal's web site is located at http://www.liebertpub.com/publication.aspx?pub_id=261en_HK
dc.relation.ispartofTissue Engineering - Part C: Methodsen_HK
dc.rightsThis is a copy of an article published in the Tissue Engineering. Part C. Methods © 2009 copyright Mary Ann Liebert, Inc.; Tissue Engineering. Part C. Methods is available online at: http://www.liebertonline.com.-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshCell Lineage - drug effects-
dc.subject.meshChondrocytes - cytology - drug effects-
dc.subject.meshCollagen - pharmacology-
dc.subject.meshExtracellular Matrix - drug effects - metabolism-
dc.subject.meshMicrospheres-
dc.titleDecellularization of chondrocyte-encapsulated collagen microspheres: A three-dimensional model to study the effects of acellular matrix on stem cell fateen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1937-3384&volume=15&issue=4&spage=697&epage=706&date=2009&atitle=Decellularization+of+chondrocyte-encapsulated+collagen+microspheres:+a+three-dimensional+model+to+study+the+effects+of+acellular+matrix+on+stem+cell+fateen_HK
dc.identifier.emailCheung, KMC:cheungmc@hku.hken_HK
dc.identifier.emailChan, D:chand@hkucc.hku.hken_HK
dc.identifier.emailChan, BP:bpchan@hkucc.hku.hken_HK
dc.identifier.authorityCheung, KMC=rp00387en_HK
dc.identifier.authorityChan, D=rp00540en_HK
dc.identifier.authorityChan, BP=rp00087en_HK
dc.description.naturepublished_or_final_versionen_US
dc.identifier.doi10.1089/ten.tec.2008.0635en_HK
dc.identifier.pmid19260803-
dc.identifier.scopuseid_2-s2.0-72249108768en_HK
dc.identifier.hkuros169431en_HK
dc.identifier.hkuros180120-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-72249108768&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume15en_HK
dc.identifier.issue4en_HK
dc.identifier.spage697en_HK
dc.identifier.epage706en_HK
dc.identifier.isiWOS:000272609100017-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectCollagen biomaterial and bone marrow derived mesenchymal stem cell (MSCs) based therapy - Second generation tissue engineering solutions for cartilage repair-
dc.identifier.scopusauthoridCheng, HW=35794981100en_HK
dc.identifier.scopusauthoridTsui, YK=7006760586en_HK
dc.identifier.scopusauthoridCheung, KMC=7402406754en_HK
dc.identifier.scopusauthoridChan, D=7402216545en_HK
dc.identifier.scopusauthoridChan, BP=7201530390en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats