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Article: Expression and protein-binding studies of the EEN gene family, new interacting partners for dynamin, synaptojanin and huntingtin proteins

TitleExpression and protein-binding studies of the EEN gene family, new interacting partners for dynamin, synaptojanin and huntingtin proteins
Authors
KeywordsEndocytosis
Endophilin family
SH3 domain
Issue Date2000
PublisherPortland Press Ltd. The Journal's web site is located at http://www.biochemj.org
Citation
Biochemical Journal, 2000, v. 348 n. 2, p. 447-458 How to Cite?
AbstractEEN, identified initially as a fusion partner to the mixed-lineage leukaemia gene in human leukaemia, and its related members, EEN-B1 and EEN-B2, have recently been shown to interact with two endocytic molecules, dynamin and synaptojanin, as well as with the huntingtin protein. In the present study, we show that the expression of the EEN gene-family members is differentially regulated. Multiple-spliced variants were identified for EEN-B2. In the brain, EEN-B1 and EEN-B2 mRNA are preferentially expressed in the cerebellar Purkinje and granule cells, dentate gyrus cells, hippocampal pyramidal neurons and cerebral granule cells. The expression patterns of EEN-B1 and EEN-B2 mRNA in the brain overlap with those of dynamin-I/III, synaptojanin-I and huntingtin, whereas the ubiquitous expression of EEN is consistent with that of dynamin-II. In testes, members of the EEN family are co-expressed with testis-type dynamin and huntingtin in Sertoli cells and germ cells respectively. Our results on the overlapping expression patterns are consistent with the proposed interaction of EEN family members with dynamin, synaptojanin and huntingtin protein in vivo. Although all three EEN family members bind to dynamin and synaptojanin, EEN-B1 has the highest affinity for binding, followed by EEN and EEN-B2. We also demonstrate that amphiphysin, a major synaptojanin-binding protein in brain, can compete with the EEN family for binding to synaptojanin and dynamin. We propose that recruitment of the EEN family by dynamin/synaptojanin to clathrin-coated pits can be regulated by amphiphysin.
Persistent Identifierhttp://hdl.handle.net/10722/68128
ISSN
2015 Impact Factor: 3.562
2015 SCImago Journal Rankings: 2.582
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSo, CWen_HK
dc.contributor.authorSham, MHen_HK
dc.contributor.authorChew, SLen_HK
dc.contributor.authorCheung, Nen_HK
dc.contributor.authorSo, CKCen_HK
dc.contributor.authorChung, SKen_HK
dc.contributor.authorCaldas, Cen_HK
dc.contributor.authorWiedemann, LMen_HK
dc.contributor.authorChan, LCen_HK
dc.date.accessioned2010-09-06T06:01:37Z-
dc.date.available2010-09-06T06:01:37Z-
dc.date.issued2000en_HK
dc.identifier.citationBiochemical Journal, 2000, v. 348 n. 2, p. 447-458en_HK
dc.identifier.issn0264-6021en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68128-
dc.description.abstractEEN, identified initially as a fusion partner to the mixed-lineage leukaemia gene in human leukaemia, and its related members, EEN-B1 and EEN-B2, have recently been shown to interact with two endocytic molecules, dynamin and synaptojanin, as well as with the huntingtin protein. In the present study, we show that the expression of the EEN gene-family members is differentially regulated. Multiple-spliced variants were identified for EEN-B2. In the brain, EEN-B1 and EEN-B2 mRNA are preferentially expressed in the cerebellar Purkinje and granule cells, dentate gyrus cells, hippocampal pyramidal neurons and cerebral granule cells. The expression patterns of EEN-B1 and EEN-B2 mRNA in the brain overlap with those of dynamin-I/III, synaptojanin-I and huntingtin, whereas the ubiquitous expression of EEN is consistent with that of dynamin-II. In testes, members of the EEN family are co-expressed with testis-type dynamin and huntingtin in Sertoli cells and germ cells respectively. Our results on the overlapping expression patterns are consistent with the proposed interaction of EEN family members with dynamin, synaptojanin and huntingtin protein in vivo. Although all three EEN family members bind to dynamin and synaptojanin, EEN-B1 has the highest affinity for binding, followed by EEN and EEN-B2. We also demonstrate that amphiphysin, a major synaptojanin-binding protein in brain, can compete with the EEN family for binding to synaptojanin and dynamin. We propose that recruitment of the EEN family by dynamin/synaptojanin to clathrin-coated pits can be regulated by amphiphysin.en_HK
dc.languageengen_HK
dc.publisherPortland Press Ltd. The Journal's web site is located at http://www.biochemj.orgen_HK
dc.relation.ispartofBiochemical Journalen_HK
dc.subjectEndocytosisen_HK
dc.subjectEndophilin familyen_HK
dc.subjectSH3 domainen_HK
dc.subject.meshAlternative Splicingen_HK
dc.subject.meshAmino Acid Sequenceen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshBrain - metabolismen_HK
dc.subject.meshCloning, Molecularen_HK
dc.subject.meshDynamin Ien_HK
dc.subject.meshDynamin IIIen_HK
dc.subject.meshDynaminsen_HK
dc.subject.meshEmbryonic and Fetal Developmenten_HK
dc.subject.meshEnzyme Inhibitors - metabolismen_HK
dc.subject.meshGTP Phosphohydrolases - metabolismen_HK
dc.subject.meshGene Expression Regulation, Developmentalen_HK
dc.subject.meshGenetic Variationen_HK
dc.subject.meshHumansen_HK
dc.subject.meshHuntington Disease - geneticsen_HK
dc.subject.meshIntracellular Signaling Peptides and Proteinsen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshMultigene Familyen_HK
dc.subject.meshNerve Tissue Proteins - metabolismen_HK
dc.subject.meshNeurons - metabolismen_HK
dc.subject.meshNuclear Proteins - metabolismen_HK
dc.subject.meshPhosphoric Monoester Hydrolases - metabolismen_HK
dc.subject.meshProtein Bindingen_HK
dc.subject.meshProteins - genetics - metabolismen_HK
dc.subject.meshRecombinant Fusion Proteins - metabolismen_HK
dc.subject.meshTranscription, Geneticen_HK
dc.titleExpression and protein-binding studies of the EEN gene family, new interacting partners for dynamin, synaptojanin and huntingtin proteinsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0264-6021&volume=348&spage=447&epage=458&date=2000&atitle=Expression+and+protein-binding+studies+of+the+EEN+gene+family,+new+interacting+partners+for+dynamin,+synaptojanin+and+huntingtin+proteinsen_HK
dc.identifier.emailSham, MH:mhsham@hkucc.hku.hken_HK
dc.identifier.emailChung, SK:skchung@hkucc.hku.hken_HK
dc.identifier.emailChan, LC:chanlc@hkucc.hku.hken_HK
dc.identifier.authoritySham, MH=rp00380en_HK
dc.identifier.authorityChung, SK=rp00381en_HK
dc.identifier.authorityChan, LC=rp00373en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1042/0264-6021:3480447en_HK
dc.identifier.pmid10816441-
dc.identifier.pmcidPMC1221085-
dc.identifier.scopuseid_2-s2.0-0034212806en_HK
dc.identifier.hkuros50825en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034212806&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume348en_HK
dc.identifier.issue2en_HK
dc.identifier.spage447en_HK
dc.identifier.epage458en_HK
dc.identifier.isiWOS:000087713800026-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridSo, CW=7102919928en_HK
dc.identifier.scopusauthoridSham, MH=7003729109en_HK
dc.identifier.scopusauthoridChew, SL=7202524605en_HK
dc.identifier.scopusauthoridCheung, N=36803314200en_HK
dc.identifier.scopusauthoridSo, CKC=7102919975en_HK
dc.identifier.scopusauthoridChung, SK=7404292976en_HK
dc.identifier.scopusauthoridCaldas, C=7006688992en_HK
dc.identifier.scopusauthoridWiedemann, LM=35467788500en_HK
dc.identifier.scopusauthoridChan, LC=7403540707en_HK

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