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Article: Mesenchymal stem cell-based repair of articular cartilage with polyglycolic acid-hydroxyapatite biphasic scaffold

TitleMesenchymal stem cell-based repair of articular cartilage with polyglycolic acid-hydroxyapatite biphasic scaffold
Authors
KeywordsCartilage
Hydroxyapatite
MSC (mesenchymal stem cell)
Polyglycolic acid
Tissue engineering
Issue Date2008
PublisherWichtig Editore srl. The Journal's web site is located at http://www.artificial-organs.com
Citation
International Journal Of Artificial Organs, 2008, v. 31 n. 6, p. 480-489 How to Cite?
AbstractThis study investigates the capacity of a composite scaffold composed of polyglycolic acid-hydroxyapatite (PGA-HA) and autologous mesenchymal stem cells (MSCs) to promote repair of osteochondral defects. MSCs from culture-expanded rabbits were seeded onto a PGA and HA scaffold. After a 72-hour co-culture period, the cell-adhered PGA and HA were joined together, forming an MSCs-PGA-HA composite. Full-thickness cartilage defects in the intercondylar fossa of the femur were then implanted with the MSC-PGA-HA composite, the PGA-HA scaffold only, or they were left empty (n=20). Animals were sacrificed 16 or 32 weeks after surgery and the gross appearance of the defects was evaluated. The specimens were examined histologically for morphologic features, and stained immunohistochemically for type 2 collagen. Specimens of the MSCs-PGA-HA composite implantation group demonstrated hyaline cartilage and a complete subchondral bone formation. At 16 weeks post-implantation, significant integration of the newly formed tissue with surrounding normal cartilage and subchondral bone was observed when compared to the two control groups. At 32 weeks, no sign of progressive degeneration of the newly formed tissue was found. A significant difference in histological grading score was found compared with the control groups. The novel MSCs-seeded, PGA-HA biphasic graft facilitated both articular cartilage and subchondral bone regeneration in an animal model and might serve as a new approach for clinical applications. © Wichtig Editore, 2008.
Persistent Identifierhttp://hdl.handle.net/10722/68127
ISSN
2015 Impact Factor: 1.005
2015 SCImago Journal Rankings: 0.585
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhou, XZen_HK
dc.contributor.authorLeung, VYen_HK
dc.contributor.authorDong, QRen_HK
dc.contributor.authorCheung, KMen_HK
dc.contributor.authorChan, Den_HK
dc.contributor.authorLu, WWen_HK
dc.date.accessioned2010-09-06T06:01:36Z-
dc.date.available2010-09-06T06:01:36Z-
dc.date.issued2008en_HK
dc.identifier.citationInternational Journal Of Artificial Organs, 2008, v. 31 n. 6, p. 480-489en_HK
dc.identifier.issn0391-3988en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68127-
dc.description.abstractThis study investigates the capacity of a composite scaffold composed of polyglycolic acid-hydroxyapatite (PGA-HA) and autologous mesenchymal stem cells (MSCs) to promote repair of osteochondral defects. MSCs from culture-expanded rabbits were seeded onto a PGA and HA scaffold. After a 72-hour co-culture period, the cell-adhered PGA and HA were joined together, forming an MSCs-PGA-HA composite. Full-thickness cartilage defects in the intercondylar fossa of the femur were then implanted with the MSC-PGA-HA composite, the PGA-HA scaffold only, or they were left empty (n=20). Animals were sacrificed 16 or 32 weeks after surgery and the gross appearance of the defects was evaluated. The specimens were examined histologically for morphologic features, and stained immunohistochemically for type 2 collagen. Specimens of the MSCs-PGA-HA composite implantation group demonstrated hyaline cartilage and a complete subchondral bone formation. At 16 weeks post-implantation, significant integration of the newly formed tissue with surrounding normal cartilage and subchondral bone was observed when compared to the two control groups. At 32 weeks, no sign of progressive degeneration of the newly formed tissue was found. A significant difference in histological grading score was found compared with the control groups. The novel MSCs-seeded, PGA-HA biphasic graft facilitated both articular cartilage and subchondral bone regeneration in an animal model and might serve as a new approach for clinical applications. © Wichtig Editore, 2008.en_HK
dc.languageengen_HK
dc.publisherWichtig Editore srl. The Journal's web site is located at http://www.artificial-organs.comen_HK
dc.relation.ispartofInternational Journal of Artificial Organsen_HK
dc.subjectCartilageen_HK
dc.subjectHydroxyapatiteen_HK
dc.subjectMSC (mesenchymal stem cell)en_HK
dc.subjectPolyglycolic aciden_HK
dc.subjectTissue engineeringen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBiocompatible Materialsen_HK
dc.subject.meshCartilage, Articular - cytology - physiologyen_HK
dc.subject.meshChondrogenesis - physiologyen_HK
dc.subject.meshCoculture Techniquesen_HK
dc.subject.meshDurapatite - pharmacologyen_HK
dc.subject.meshImmunoenzyme Techniquesen_HK
dc.subject.meshKnee Jointen_HK
dc.subject.meshMesenchymal Stem Cell Transplantationen_HK
dc.subject.meshMesenchymal Stem Cells - drug effects - physiologyen_HK
dc.subject.meshMicroscopy, Electron, Scanningen_HK
dc.subject.meshPolyglycolic Acid - pharmacologyen_HK
dc.subject.meshRabbitsen_HK
dc.subject.meshTissue Engineering - methodsen_HK
dc.subject.meshTissue Scaffoldsen_HK
dc.titleMesenchymal stem cell-based repair of articular cartilage with polyglycolic acid-hydroxyapatite biphasic scaffolden_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0391-3988&volume=6&spage=480&epage=9&date=2008&atitle=Mesenchymal+stem+cell-based+repair+of+articular+cartilage+with+polyglycolic+acid-hydroxyapatite+biphasic+scaffolden_HK
dc.identifier.emailLeung, VY: vicleung@hku.hken_HK
dc.identifier.emailCheung, KM: cheungmc@hku.hken_HK
dc.identifier.emailChan, D: chand@hkucc.hku.hken_HK
dc.identifier.emailLu, WW: wwlu@hku.hken_HK
dc.identifier.authorityLeung, VY=rp01764en_HK
dc.identifier.authorityCheung, KM=rp00387en_HK
dc.identifier.authorityChan, D=rp00540en_HK
dc.identifier.authorityLu, WW=rp00411en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid18609500-
dc.identifier.scopuseid_2-s2.0-49649129685en_HK
dc.identifier.hkuros151260en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-49649129685&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume31en_HK
dc.identifier.issue6en_HK
dc.identifier.spage480en_HK
dc.identifier.epage489en_HK
dc.identifier.isiWOS:000258458100003-
dc.publisher.placeItalyen_HK
dc.identifier.scopusauthoridZhou, XZ=7410093508en_HK
dc.identifier.scopusauthoridLeung, VY=35337438900en_HK
dc.identifier.scopusauthoridDong, QR=7201748862en_HK
dc.identifier.scopusauthoridCheung, KM=7402406754en_HK
dc.identifier.scopusauthoridChan, D=7402216545en_HK
dc.identifier.scopusauthoridLu, WW=55484358100en_HK

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