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Article: Segmental regulation of Hoxb-3 by kreisler

TitleSegmental regulation of Hoxb-3 by kreisler
Authors
Issue Date1997
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/nature
Citation
Nature, 1997, v. 387 n. 6629, p. 191-195 How to Cite?
AbstractHox genes control regional identity during segmentation of the vertebrate hindbrain into rhombomeres. Here we use transgenic analysis to investigate the upstream mechanisms for regulation of Hoxb-3 in rhombomere(r)5. We identified enhancers from the mouse and chick genes sufficient for r5-restricted expression. Sequence comparisons revealed two blocks of similarity (of 19 and 45 base pairs), which each contain in vitro binding sites for the kreisler protein (Krml1), a Maf/b-Zip protein expressed in r5 and r6 (ref. 4). Both sites are required for r5 activity, suggesting that Hoxb-3 is a direct target of kreisler. Multimers of the 19-base-pair (bp) block recreate a Krml1-like pattern in r5/r6, but the 45-bp block mediates expression only in r5. Therefore elements within the 45-bp block restrict the response to Krml1. We identified additional sequences that contain an Ets-related activation site, required for both the activation and restriction to r5. These studies demonstrate that Krml1 directly activates expression of Hoxb-3 in r5 in combination with an Ets-related activation site, and suggest that kreisler plays a primary role in regulating segmental identity through Hox genes.
Persistent Identifierhttp://hdl.handle.net/10722/68120
ISSN
2015 Impact Factor: 38.138
2015 SCImago Journal Rankings: 21.936
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorManzanares, Men_HK
dc.contributor.authorCordes, Sen_HK
dc.contributor.authorKwan, CTen_HK
dc.contributor.authorSham, MHen_HK
dc.contributor.authorBarsh, GSen_HK
dc.contributor.authorKrumlauf, Ren_HK
dc.date.accessioned2010-09-06T06:01:32Z-
dc.date.available2010-09-06T06:01:32Z-
dc.date.issued1997en_HK
dc.identifier.citationNature, 1997, v. 387 n. 6629, p. 191-195en_HK
dc.identifier.issn0028-0836en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68120-
dc.description.abstractHox genes control regional identity during segmentation of the vertebrate hindbrain into rhombomeres. Here we use transgenic analysis to investigate the upstream mechanisms for regulation of Hoxb-3 in rhombomere(r)5. We identified enhancers from the mouse and chick genes sufficient for r5-restricted expression. Sequence comparisons revealed two blocks of similarity (of 19 and 45 base pairs), which each contain in vitro binding sites for the kreisler protein (Krml1), a Maf/b-Zip protein expressed in r5 and r6 (ref. 4). Both sites are required for r5 activity, suggesting that Hoxb-3 is a direct target of kreisler. Multimers of the 19-base-pair (bp) block recreate a Krml1-like pattern in r5/r6, but the 45-bp block mediates expression only in r5. Therefore elements within the 45-bp block restrict the response to Krml1. We identified additional sequences that contain an Ets-related activation site, required for both the activation and restriction to r5. These studies demonstrate that Krml1 directly activates expression of Hoxb-3 in r5 in combination with an Ets-related activation site, and suggest that kreisler plays a primary role in regulating segmental identity through Hox genes.en_HK
dc.languageengen_HK
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/natureen_HK
dc.relation.ispartofNatureen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAvian Proteinsen_HK
dc.subject.meshBinding Sitesen_HK
dc.subject.meshChick Embryoen_HK
dc.subject.meshDNA-Binding Proteins - genetics - metabolismen_HK
dc.subject.meshEnhancer Elements, Geneticen_HK
dc.subject.meshGene Expression Regulation, Developmentalen_HK
dc.subject.meshGenes, Homeoboxen_HK
dc.subject.meshHomeodomain Proteins - geneticsen_HK
dc.subject.meshLeucine Zippersen_HK
dc.subject.meshMafB Transcription Factoren_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Transgenicen_HK
dc.subject.meshOncogene Proteinsen_HK
dc.subject.meshProto-Oncogene Proteins - genetics - metabolismen_HK
dc.subject.meshProto-Oncogene Proteins c-etsen_HK
dc.subject.meshRhombencephalon - embryology - metabolismen_HK
dc.subject.meshSequence Deletionen_HK
dc.subject.meshTranscription Factors - genetics - metabolismen_HK
dc.subject.meshUp-Regulationen_HK
dc.subject.meshXenopus Proteinsen_HK
dc.titleSegmental regulation of Hoxb-3 by kreisleren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0028-0836&volume=387&spage=191&epage=195&date=1997&atitle=Segmental+regulation+of+Hoxb-3+by+Kreisleren_HK
dc.identifier.emailSham, MH:mhsham@hkucc.hku.hken_HK
dc.identifier.authoritySham, MH=rp00380en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1038/387191a0en_HK
dc.identifier.pmid9144291-
dc.identifier.scopuseid_2-s2.0-0030906395en_HK
dc.identifier.hkuros25878en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030906395&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume387en_HK
dc.identifier.issue6629en_HK
dc.identifier.spage191en_HK
dc.identifier.epage195en_HK
dc.identifier.isiWOS:A1997WX94500057-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridManzanares, M=7003611983en_HK
dc.identifier.scopusauthoridCordes, S=7005622768en_HK
dc.identifier.scopusauthoridKwan, CT=7201421142en_HK
dc.identifier.scopusauthoridSham, MH=7003729109en_HK
dc.identifier.scopusauthoridBarsh, GS=35394756500en_HK
dc.identifier.scopusauthoridKrumlauf, R=8874137700en_HK

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