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Article: G-box binding coincides with increased Solanum melongena cysteine proteinase expression in senescent fruits and circadian-regulated leaves

TitleG-box binding coincides with increased Solanum melongena cysteine proteinase expression in senescent fruits and circadian-regulated leaves
Authors
KeywordsCis-elements
Clock
DNase I footprinting
Eggplant
Ethylene
Issue Date2003
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0167-4412
Citation
Plant Molecular Biology, 2003, v. 51 n. 1, p. 9-19 How to Cite?
AbstractWe have previously shown that SmCP, the gene encoding Solanum melongena cysteine proteinase, is expressed during developmental events associated with programmed cell death (PCD) suggesting its involvement in protein degradation during these events (Xu and Chye, Plant Journal 17 (1999) 321-327). Here, we investigated the regulation of SmCP expression and showed that it is ethylene-inducible and is under circadian control. This circadian rhythm is entrained by light/dark (LD) cycling with peak expression in the late light period, as opposed to that in early light for rbcS, suggesting that protein degradation and photosynthesis are temporally separated by circadian control. Northern blot analysis shows that the pattern of ethylene induction of SmCP is consistent with our previous observation of its significantly increased expression at leaf senescence and fruit ripening when endogenous ethylene is abundant. To further understand SmCP regulation, we have cloned the SmCP promoter and identified a G-box (CACGTG) at -85/-80 by DNase I footprinting analysis of the -221/+17 region. Its specific interaction with nuclear proteins in S. melongena leaves and fruits was confirmed by competitive electrophoretic mobility shift assays using oligonucleotides containing the G-box and mutant derivatives. G-box binding activity was stronger in senescent than young fruits. In circadian-regulated leaves, stronger binding activity coincided with peak circadian expression of SmCP. This correlation between binding activity and expression suggests that G-box binding factors enhance SmCP transcription and that the G-box likely plays a role in circadian regulation of genes affected by LD cycling.
Persistent Identifierhttp://hdl.handle.net/10722/68119
ISSN
2015 Impact Factor: 3.905
2015 SCImago Journal Rankings: 1.915
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorXu, ZFen_HK
dc.contributor.authorChye, MLen_HK
dc.contributor.authorLi, HYen_HK
dc.contributor.authorXu, FXen_HK
dc.contributor.authorYao, KMen_HK
dc.date.accessioned2010-09-06T06:01:31Z-
dc.date.available2010-09-06T06:01:31Z-
dc.date.issued2003en_HK
dc.identifier.citationPlant Molecular Biology, 2003, v. 51 n. 1, p. 9-19en_HK
dc.identifier.issn0167-4412en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68119-
dc.description.abstractWe have previously shown that SmCP, the gene encoding Solanum melongena cysteine proteinase, is expressed during developmental events associated with programmed cell death (PCD) suggesting its involvement in protein degradation during these events (Xu and Chye, Plant Journal 17 (1999) 321-327). Here, we investigated the regulation of SmCP expression and showed that it is ethylene-inducible and is under circadian control. This circadian rhythm is entrained by light/dark (LD) cycling with peak expression in the late light period, as opposed to that in early light for rbcS, suggesting that protein degradation and photosynthesis are temporally separated by circadian control. Northern blot analysis shows that the pattern of ethylene induction of SmCP is consistent with our previous observation of its significantly increased expression at leaf senescence and fruit ripening when endogenous ethylene is abundant. To further understand SmCP regulation, we have cloned the SmCP promoter and identified a G-box (CACGTG) at -85/-80 by DNase I footprinting analysis of the -221/+17 region. Its specific interaction with nuclear proteins in S. melongena leaves and fruits was confirmed by competitive electrophoretic mobility shift assays using oligonucleotides containing the G-box and mutant derivatives. G-box binding activity was stronger in senescent than young fruits. In circadian-regulated leaves, stronger binding activity coincided with peak circadian expression of SmCP. This correlation between binding activity and expression suggests that G-box binding factors enhance SmCP transcription and that the G-box likely plays a role in circadian regulation of genes affected by LD cycling.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0167-4412en_HK
dc.relation.ispartofPlant Molecular Biologyen_HK
dc.subjectCis-elementsen_HK
dc.subjectClocken_HK
dc.subjectDNase I footprintingen_HK
dc.subjectEggplanten_HK
dc.subjectEthyleneen_HK
dc.subject.meshAgingen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshCircadian Rhythm - physiologyen_HK
dc.subject.meshCysteine Endopeptidases - geneticsen_HK
dc.subject.meshDNA Primersen_HK
dc.subject.meshGene Expression Regulation, Enzymologicen_HK
dc.subject.meshGene Expression Regulation, Planten_HK
dc.subject.meshGenomic Libraryen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshPlant Leaves - growth & development - physiologyen_HK
dc.subject.meshPromoter Regions, Geneticen_HK
dc.subject.meshRNA, Messenger - geneticsen_HK
dc.subject.meshSeeds - physiologyen_HK
dc.subject.meshSequence Alignmenten_HK
dc.subject.meshSequence Homology, Nucleic Aciden_HK
dc.subject.meshSolanum - enzymology - genetics - growth & developmenten_HK
dc.titleG-box binding coincides with increased Solanum melongena cysteine proteinase expression in senescent fruits and circadian-regulated leavesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0167-4412&volume=51&spage=9&epage=19&date=2003&atitle=G-box+binding+coincides+with+increased+solanum+melongena+cysteine+proteinase+expression+in+senescent+fruits+and+circadian-regulated+leavesen_HK
dc.identifier.emailChye, ML: mlchye@hkucc.hku.hken_HK
dc.identifier.emailYao, KM: kmyao@hku.hken_HK
dc.identifier.authorityChye, ML=rp00687en_HK
dc.identifier.authorityYao, KM=rp00344en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1023/A:1020859518877en_HK
dc.identifier.pmid12602887-
dc.identifier.scopuseid_2-s2.0-0037278686en_HK
dc.identifier.hkuros84835en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037278686&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume51en_HK
dc.identifier.issue1en_HK
dc.identifier.spage9en_HK
dc.identifier.epage19en_HK
dc.identifier.isiWOS:000178667200002-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridXu, ZF=7405425506en_HK
dc.identifier.scopusauthoridChye, ML=7003905460en_HK
dc.identifier.scopusauthoridLi, HY=22953303900en_HK
dc.identifier.scopusauthoridXu, FX=7401695108en_HK
dc.identifier.scopusauthoridYao, KM=7403234578en_HK

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