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Article: Mechanisms associated with IL-6-induced up-regulation of Jak3 and its role in monocytic differentiation

TitleMechanisms associated with IL-6-induced up-regulation of Jak3 and its role in monocytic differentiation
Authors
Issue Date2004
PublisherAmerican Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/
Citation
Blood, 2004, v. 103 n. 11, p. 4093-4101 How to Cite?
AbstractWe report here that Janus kinase 3 (Jak3) is a primary response gene for interleukin-6 (IL-6) in macrophage differentiation, and ectopic overexpression of Jak3 accelerates monocytic differentiation of normal mouse bone marrow cells stimulated with cytokines. Furthermore, we show that incubation of normal mouse bone marrow cells with a JAK3-specific inhibitor results in profound inhibition of myeloid colony formation in response to granulocyte-macrophage colony-stimulating factor or the combination of stem cell factor, IL-3, and IL-6. In addition, mutagenesis of the Jak3 promoter has revealed that Sp1 binding sites within a -67 to -85 element and a signal transducer and activator of transcription (Stat) binding site at position -44 to -53 are critical for activation of Jak3 transcription in murine M1 myeloid leukemia cells stimulated with IL-6. Electrophoretic mobility shift assay (EMSA) analysis has demonstrated that Sp1 can bind to the -67 to -85 element and Stat3 can bind to the -44 to -53 STAT site in IL-6-stimulated M1 cells. Additionally, ectopic overexpression of Stat3 enhanced Jak3 promoter activity in M1 cells. This mechanism of activation of the murine Jak3 promoter in myeloid cells is distinct from a recently reported mechanism of activation of the human JAK3 promoter in activated T cells. © 2004 by The American Society of Hematology.
Persistent Identifierhttp://hdl.handle.net/10722/68109
ISSN
2015 Impact Factor: 11.841
2015 SCImago Journal Rankings: 6.395
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMangan, JKen_HK
dc.contributor.authorRane, SGen_HK
dc.contributor.authorKang, ADen_HK
dc.contributor.authorAmanullah, Aen_HK
dc.contributor.authorWong, BCen_HK
dc.contributor.authorReddy, EPen_HK
dc.date.accessioned2010-09-06T06:01:25Z-
dc.date.available2010-09-06T06:01:25Z-
dc.date.issued2004en_HK
dc.identifier.citationBlood, 2004, v. 103 n. 11, p. 4093-4101en_HK
dc.identifier.issn0006-4971en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68109-
dc.description.abstractWe report here that Janus kinase 3 (Jak3) is a primary response gene for interleukin-6 (IL-6) in macrophage differentiation, and ectopic overexpression of Jak3 accelerates monocytic differentiation of normal mouse bone marrow cells stimulated with cytokines. Furthermore, we show that incubation of normal mouse bone marrow cells with a JAK3-specific inhibitor results in profound inhibition of myeloid colony formation in response to granulocyte-macrophage colony-stimulating factor or the combination of stem cell factor, IL-3, and IL-6. In addition, mutagenesis of the Jak3 promoter has revealed that Sp1 binding sites within a -67 to -85 element and a signal transducer and activator of transcription (Stat) binding site at position -44 to -53 are critical for activation of Jak3 transcription in murine M1 myeloid leukemia cells stimulated with IL-6. Electrophoretic mobility shift assay (EMSA) analysis has demonstrated that Sp1 can bind to the -67 to -85 element and Stat3 can bind to the -44 to -53 STAT site in IL-6-stimulated M1 cells. Additionally, ectopic overexpression of Stat3 enhanced Jak3 promoter activity in M1 cells. This mechanism of activation of the murine Jak3 promoter in myeloid cells is distinct from a recently reported mechanism of activation of the human JAK3 promoter in activated T cells. © 2004 by The American Society of Hematology.en_HK
dc.languageengen_HK
dc.publisherAmerican Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/en_HK
dc.relation.ispartofBlooden_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBone Marrow Cells - cytology - drug effects - physiologyen_HK
dc.subject.meshCell Differentiation - drug effects - physiologyen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshDNA Mutational Analysisen_HK
dc.subject.meshDNA-Binding Proteins - metabolismen_HK
dc.subject.meshElectrophoretic Mobility Shift Assayen_HK
dc.subject.meshGene Deletionen_HK
dc.subject.meshGene Expression - physiologyen_HK
dc.subject.meshGranulocyte-Macrophage Colony-Stimulating Factor - pharmacologyen_HK
dc.subject.meshInterleukin-6 - pharmacologyen_HK
dc.subject.meshJanus Kinase 3en_HK
dc.subject.meshMacrophages - cytology - drug effects - physiologyen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Inbred C57BLen_HK
dc.subject.meshMonocytes - cytology - drug effects - physiologyen_HK
dc.subject.meshPromoter Regions, Genetic - physiologyen_HK
dc.subject.meshProtein-Tyrosine Kinases - geneticsen_HK
dc.subject.meshSTAT3 Transcription Factoren_HK
dc.subject.meshSp1 Transcription Factor - metabolismen_HK
dc.subject.meshStem Cells - cytology - physiologyen_HK
dc.subject.meshTrans-Activators - metabolismen_HK
dc.subject.meshUp-Regulation - drug effects - physiologyen_HK
dc.titleMechanisms associated with IL-6-induced up-regulation of Jak3 and its role in monocytic differentiationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-4971&volume=103&issue=11&spage=4093&epage=101&date=2004&atitle=Mechanisms+associated+with+IL-6-induced+up-regulation+of+Jak3+and+its+role+in+monocytic+differentiation.en_HK
dc.identifier.emailWong, BC:bcwwong@hkucc.hku.hken_HK
dc.identifier.authorityWong, BC=rp00369en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1182/blood-2003-06-2165en_HK
dc.identifier.pmid14976041-
dc.identifier.scopuseid_2-s2.0-2542480822en_HK
dc.identifier.hkuros88746en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-2542480822&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume103en_HK
dc.identifier.issue11en_HK
dc.identifier.spage4093en_HK
dc.identifier.epage4101en_HK
dc.identifier.isiWOS:000221657600024-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridMangan, JK=7005389031en_HK
dc.identifier.scopusauthoridRane, SG=7005884921en_HK
dc.identifier.scopusauthoridKang, AD=8084356400en_HK
dc.identifier.scopusauthoridAmanullah, A=7005005869en_HK
dc.identifier.scopusauthoridWong, BC=35733052400en_HK
dc.identifier.scopusauthoridReddy, EP=7101612004en_HK

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