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Article: Identification and Characterization of Human VCY2-Interacting Protein: VCY2IP-1, a Microtubule-Associated Protein-Like Protein

TitleIdentification and Characterization of Human VCY2-Interacting Protein: VCY2IP-1, a Microtubule-Associated Protein-Like Protein
Authors
KeywordsTestis
Issue Date2004
PublisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/
Citation
Biology Of Reproduction, 2004, v. 70 n. 3, p. 775-784 How to Cite?
AbstractVCY2 is a testis-specific protein that locates in a frequently deleted azoospermia factor c region on chromosome Yq. Although its genomic structure has been characterized, the function of VCY2 is still unknown. To gain insight regarding the likely function of VCY2, we investigated the proteins that interact with VCY2 using the yeast two-hybrid system. We identified a novel VCY2 interaction partner, named VCY2IP-1, that encodes an open reading frame of 1059 amino acids. The amino acid sequence of VCY2IP-1 shows 59.3% and 41.9% homology to two human microtubule-associated proteins (MAPs), MAP1B and MAP1A, respectively. VCY2IP-1 has an extensive homology to the N-terminus and C-terminus regions of MAP1B and MAP1A, placing it within a large family of MAPs. We mapped VCY2IP-1 to chromosome 19p13.11. The VCY2IP-1 gene spans 15 kilobases (kb) and consists of seven exons. Northern blot analysis identified a single, intense band of approximately 3.2-kb VCY2IP-1 transcript, predominantly expressed in human testis. In situ hybridization of human testicular sections showed the localization of VCY2IP-1 transcripts in germ cells, and reverse transcription-polymerase chain reaction analysis demonstrated the presence of VCY2 and VCY2IP-1 transcripts in human ejaculated spermatozoa. Our expression data support the involvement of VCY2 and VCY2IP-1 in spermatogenesis. Based on the high homology of VCY2IP-1 with MAPs, we propose the involvement of VCY2 in the cytoskeletal network via interaction with VCY2IP-1.
Persistent Identifierhttp://hdl.handle.net/10722/68065
ISSN
2015 Impact Factor: 3.471
2015 SCImago Journal Rankings: 1.646
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, EYMen_HK
dc.contributor.authorTse, JYMen_HK
dc.contributor.authorYao, KMen_HK
dc.contributor.authorLui, VCHen_HK
dc.contributor.authorTam, PCen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2010-09-06T06:01:00Z-
dc.date.available2010-09-06T06:01:00Z-
dc.date.issued2004en_HK
dc.identifier.citationBiology Of Reproduction, 2004, v. 70 n. 3, p. 775-784en_HK
dc.identifier.issn0006-3363en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68065-
dc.description.abstractVCY2 is a testis-specific protein that locates in a frequently deleted azoospermia factor c region on chromosome Yq. Although its genomic structure has been characterized, the function of VCY2 is still unknown. To gain insight regarding the likely function of VCY2, we investigated the proteins that interact with VCY2 using the yeast two-hybrid system. We identified a novel VCY2 interaction partner, named VCY2IP-1, that encodes an open reading frame of 1059 amino acids. The amino acid sequence of VCY2IP-1 shows 59.3% and 41.9% homology to two human microtubule-associated proteins (MAPs), MAP1B and MAP1A, respectively. VCY2IP-1 has an extensive homology to the N-terminus and C-terminus regions of MAP1B and MAP1A, placing it within a large family of MAPs. We mapped VCY2IP-1 to chromosome 19p13.11. The VCY2IP-1 gene spans 15 kilobases (kb) and consists of seven exons. Northern blot analysis identified a single, intense band of approximately 3.2-kb VCY2IP-1 transcript, predominantly expressed in human testis. In situ hybridization of human testicular sections showed the localization of VCY2IP-1 transcripts in germ cells, and reverse transcription-polymerase chain reaction analysis demonstrated the presence of VCY2 and VCY2IP-1 transcripts in human ejaculated spermatozoa. Our expression data support the involvement of VCY2 and VCY2IP-1 in spermatogenesis. Based on the high homology of VCY2IP-1 with MAPs, we propose the involvement of VCY2 in the cytoskeletal network via interaction with VCY2IP-1.en_HK
dc.languageengen_HK
dc.publisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/en_HK
dc.relation.ispartofBiology of Reproductionen_HK
dc.subjectTestisen_HK
dc.subject.meshAmino Acid Sequenceen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshChromosomes, Human, Pair 19en_HK
dc.subject.meshGene Libraryen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunoprecipitationen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMicrotubule-Associated Proteins - genetics - metabolismen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshProteins - metabolismen_HK
dc.subject.meshRNA, Messenger - analysisen_HK
dc.subject.meshSequence Homology, Amino Aciden_HK
dc.subject.meshSpermatozoa - physiologyen_HK
dc.subject.meshTestis - physiologyen_HK
dc.subject.meshTwo-Hybrid System Techniquesen_HK
dc.subject.meshYeasts - physiologyen_HK
dc.titleIdentification and Characterization of Human VCY2-Interacting Protein: VCY2IP-1, a Microtubule-Associated Protein-Like Proteinen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-3363&volume=70&spage=775&epage=784&date=2004&atitle=Identification+and+characterization+of+human+VCY2-interacting+protein:+VCY2IP-1,+a+microtubule-associated+protein-like+proteinen_HK
dc.identifier.emailWong, EYM: elainewg@hku.hken_HK
dc.identifier.emailYao, KM: kmyao@hku.hken_HK
dc.identifier.emailLui, VCH: vchlui@hku.hken_HK
dc.identifier.emailYeung, WSB: wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityWong, EYM=rp01718en_HK
dc.identifier.authorityYao, KM=rp00344en_HK
dc.identifier.authorityLui, VCH=rp00363en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1095/biolreprod.103.018531en_HK
dc.identifier.pmid14627543-
dc.identifier.scopuseid_2-s2.0-1242345152en_HK
dc.identifier.hkuros106314en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-1242345152&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume70en_HK
dc.identifier.issue3en_HK
dc.identifier.spage775en_HK
dc.identifier.epage784en_HK
dc.identifier.isiWOS:000189150400030-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWong, EYM=36719382700en_HK
dc.identifier.scopusauthoridTse, JYM=7102607314en_HK
dc.identifier.scopusauthoridYao, KM=7403234578en_HK
dc.identifier.scopusauthoridLui, VCH=7004231344en_HK
dc.identifier.scopusauthoridTam, PC=7202539419en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK
dc.identifier.citeulike9922670-

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