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Article: Mapping heparanase expression in the spinal cord of adult rats

TitleMapping heparanase expression in the spinal cord of adult rats
Authors
KeywordsAstrocytes
Heparan sulfate proteoglycans
Neurons
NG2-expressing glia
Oligodendrocytes
Syndecan
Issue Date2006
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31248
Citation
Journal Of Comparative Neurology, 2006, v. 494 n. 2, p. 345-357 How to Cite?
AbstractThis work addresses the cellular localization of heparanase and its colocalization with syndecan-3, a transmembrane heparan sulfate proteoglycan in spinal cords of adult rats. Reverse transcriptase/polymerase chain reaction (RT-PCR) and in situ hybridization for the heparanase transcript revealed expression in neurons and white matter glia. This was confirmed by immunohistochemistry showing cytoplasmic localization of the heparanase protein. Double immunofluorescence for heparanase and syndecan-3 revealed colocalization of the proteins in cell bodies of neurons and oligodendrocytes, suggestive of constitutive expression in these cell types. In contrast, only subpopulations of astrocytes and NG2-expressing glia in the white matter expressed heparanase, and these did not show expression of syndecan-3. Cultures of astrocytes further evidenced upregulation of heparanase expression with TGF-β1 treatment, but no accompanying upregulation of syndecan-3 was detectable. These first findings of heparanase expression in the adult cord therefore provide the cellular basis for understanding functional interactions of heparanase and syndecan-3 in the normal neural network or otherwise in glial reactions to spinal cord injury. © 2005 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/68044
ISSN
2015 Impact Factor: 3.331
2015 SCImago Journal Rankings: 2.345
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhang, Yen_HK
dc.contributor.authorYeung, MNen_HK
dc.contributor.authorLiu, Jen_HK
dc.contributor.authorChau, CHen_HK
dc.contributor.authorChan, YSen_HK
dc.contributor.authorShum, DKYen_HK
dc.date.accessioned2010-09-06T06:00:48Z-
dc.date.available2010-09-06T06:00:48Z-
dc.date.issued2006en_HK
dc.identifier.citationJournal Of Comparative Neurology, 2006, v. 494 n. 2, p. 345-357en_HK
dc.identifier.issn0021-9967en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68044-
dc.description.abstractThis work addresses the cellular localization of heparanase and its colocalization with syndecan-3, a transmembrane heparan sulfate proteoglycan in spinal cords of adult rats. Reverse transcriptase/polymerase chain reaction (RT-PCR) and in situ hybridization for the heparanase transcript revealed expression in neurons and white matter glia. This was confirmed by immunohistochemistry showing cytoplasmic localization of the heparanase protein. Double immunofluorescence for heparanase and syndecan-3 revealed colocalization of the proteins in cell bodies of neurons and oligodendrocytes, suggestive of constitutive expression in these cell types. In contrast, only subpopulations of astrocytes and NG2-expressing glia in the white matter expressed heparanase, and these did not show expression of syndecan-3. Cultures of astrocytes further evidenced upregulation of heparanase expression with TGF-β1 treatment, but no accompanying upregulation of syndecan-3 was detectable. These first findings of heparanase expression in the adult cord therefore provide the cellular basis for understanding functional interactions of heparanase and syndecan-3 in the normal neural network or otherwise in glial reactions to spinal cord injury. © 2005 Wiley-Liss, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31248en_HK
dc.relation.ispartofJournal of Comparative Neurologyen_HK
dc.rightsThe Journal of Comparative Neurology. Copyright © John Wiley & Sons, Inc.en_HK
dc.subjectAstrocytesen_HK
dc.subjectHeparan sulfate proteoglycansen_HK
dc.subjectNeuronsen_HK
dc.subjectNG2-expressing gliaen_HK
dc.subjectOligodendrocytesen_HK
dc.subjectSyndecanen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAstrocytes - cytology - metabolismen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGlucuronidase - genetics - metabolismen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshIn Situ Hybridizationen_HK
dc.subject.meshMembrane Glycoproteins - metabolismen_HK
dc.subject.meshNeurons - cytology - metabolismen_HK
dc.subject.meshProteoglycans - metabolismen_HK
dc.subject.meshRNA, Messenger - metabolismen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.subject.meshSpinal Cord - cytology - enzymologyen_HK
dc.subject.meshSyndecan-3en_HK
dc.titleMapping heparanase expression in the spinal cord of adult ratsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9967&volume=494&spage=345&epage=357&date=2006&atitle=Mapping+heparanase+expression+in+the+spinal+cord+of+adult+ratsen_HK
dc.identifier.emailChau, CH: mchchau@hkucc.hku.hken_HK
dc.identifier.emailChan, YS: yschan@hkucc.hku.hken_HK
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hken_HK
dc.identifier.authorityChau, CH=rp00398en_HK
dc.identifier.authorityChan, YS=rp00318en_HK
dc.identifier.authorityShum, DKY=rp00321en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/cne.20811en_HK
dc.identifier.pmid16320243-
dc.identifier.scopuseid_2-s2.0-29144462669en_HK
dc.identifier.hkuros137641en_HK
dc.identifier.hkuros123913-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-29144462669&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume494en_HK
dc.identifier.issue2en_HK
dc.identifier.spage345en_HK
dc.identifier.epage357en_HK
dc.identifier.isiWOS:000234026900010-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridZhang, Y=22995994100en_HK
dc.identifier.scopusauthoridYeung, MN=10043540800en_HK
dc.identifier.scopusauthoridLiu, J=36071959600en_HK
dc.identifier.scopusauthoridChau, CH=10040110500en_HK
dc.identifier.scopusauthoridChan, YS=7403676627en_HK
dc.identifier.scopusauthoridShum, DKY=7004824447en_HK

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