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Article: Expression of collagen and aggrecan genes in normal and osteoarthritic murine knee joints

TitleExpression of collagen and aggrecan genes in normal and osteoarthritic murine knee joints
Authors
Issue Date2002
PublisherWB Saunders Co Ltd. The Journal's web site is located at http://www.elsevier.com/locate/joca
Citation
Osteoarthritis And Cartilage, 2002, v. 10 n. 1, p. 51-61 How to Cite?
AbstractObjective: The STR/ort mouse strain develops osteoarthritis (OA) of the medial tibial cartilage whilst CBA mice do not develop this disease. We investigated whether changes occur in the expression of genes encoding major extracellular matrix proteins in the connective tissue of the murine knee joint in OA. Design: Expression of the genes encoding collagens II (Col2α1), X (Col10α1), α2(XI) (Col11α2) and aggrecan (Agc) was detected in skeletally mature and immature male mice of the CBA and STR/ort strains by in situ hybridization. Results: Col2α1 was expressed by chondrocytes of the tibial and patella-femoral cartilage and by the meniscal cartilage in all young mice (4-9 weeks) but only in the patella-femoral cartilage in older mice of both strains (36-45 weeks). In contrast Col2α1 was expressed by growth plate chondrocytes of both species at all ages. Similarly, Col2α1 transcripts were detected in cruciate ligament cells in both strains at all ages. Col10α1 transcripts were detected in cruciate ligament cells in both strains at all ages. Col10α1 expression was evident in the hypertrophic chondrocytes in the growth plate of young CBA and STR mice, but was not active in these cells in mature animals. However, Col10α1 was transcribed in articular chondrocytes of the tibia, meniscal and patella-femoral cartilages of all ages, in normal and osteoarthritic mice. Transcripts were also present in ligament of some mature animals. Col11α2 followed a similar pattern of expression in CBA cartilages to Col2α1, being active in adult growth plate but generally inactive in adult articular cartilages. Young CBA and STR/ort mice expressed Col11α2 in articular cartilage and very strongly throughout the growth plate. Agc expression was detected in all articular cartilages at all ages in both strains. Interestingly, transcripts for all four genes were absent in tibial articular chondrocytes located close to osteoarthritic lesions in STR/ort mice, indicating that these cells are unable to synthesize matrix proteins. Adult STR/ort mice also showed evidence of tissue remodeling around the periphery of the knee joint. Cells in remodeling areas actively transcribed Col2α1, Col10α1, Col11α2 and Agc. Conclusion: It is unlikely that OA develops in STR/ort mice because of failure to express major proteins in joint tissue. However, once lesions develop in articular cartilage neighbouring chondrocytes fail to express genes encoding several matrix proteins. © 2002 OsteoArthritis Research Society International.
Persistent Identifierhttp://hdl.handle.net/10722/68033
ISSN
2015 Impact Factor: 4.535
2015 SCImago Journal Rankings: 1.901
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChambers, MGen_HK
dc.contributor.authorKuffner, Ten_HK
dc.contributor.authorCowan, SKen_HK
dc.contributor.authorCheah, KSEen_HK
dc.contributor.authorMason, RMen_HK
dc.date.accessioned2010-09-06T06:00:42Z-
dc.date.available2010-09-06T06:00:42Z-
dc.date.issued2002en_HK
dc.identifier.citationOsteoarthritis And Cartilage, 2002, v. 10 n. 1, p. 51-61en_HK
dc.identifier.issn1063-4584en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68033-
dc.description.abstractObjective: The STR/ort mouse strain develops osteoarthritis (OA) of the medial tibial cartilage whilst CBA mice do not develop this disease. We investigated whether changes occur in the expression of genes encoding major extracellular matrix proteins in the connective tissue of the murine knee joint in OA. Design: Expression of the genes encoding collagens II (Col2α1), X (Col10α1), α2(XI) (Col11α2) and aggrecan (Agc) was detected in skeletally mature and immature male mice of the CBA and STR/ort strains by in situ hybridization. Results: Col2α1 was expressed by chondrocytes of the tibial and patella-femoral cartilage and by the meniscal cartilage in all young mice (4-9 weeks) but only in the patella-femoral cartilage in older mice of both strains (36-45 weeks). In contrast Col2α1 was expressed by growth plate chondrocytes of both species at all ages. Similarly, Col2α1 transcripts were detected in cruciate ligament cells in both strains at all ages. Col10α1 transcripts were detected in cruciate ligament cells in both strains at all ages. Col10α1 expression was evident in the hypertrophic chondrocytes in the growth plate of young CBA and STR mice, but was not active in these cells in mature animals. However, Col10α1 was transcribed in articular chondrocytes of the tibia, meniscal and patella-femoral cartilages of all ages, in normal and osteoarthritic mice. Transcripts were also present in ligament of some mature animals. Col11α2 followed a similar pattern of expression in CBA cartilages to Col2α1, being active in adult growth plate but generally inactive in adult articular cartilages. Young CBA and STR/ort mice expressed Col11α2 in articular cartilage and very strongly throughout the growth plate. Agc expression was detected in all articular cartilages at all ages in both strains. Interestingly, transcripts for all four genes were absent in tibial articular chondrocytes located close to osteoarthritic lesions in STR/ort mice, indicating that these cells are unable to synthesize matrix proteins. Adult STR/ort mice also showed evidence of tissue remodeling around the periphery of the knee joint. Cells in remodeling areas actively transcribed Col2α1, Col10α1, Col11α2 and Agc. Conclusion: It is unlikely that OA develops in STR/ort mice because of failure to express major proteins in joint tissue. However, once lesions develop in articular cartilage neighbouring chondrocytes fail to express genes encoding several matrix proteins. © 2002 OsteoArthritis Research Society International.en_HK
dc.languageengen_HK
dc.publisherWB Saunders Co Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jocaen_HK
dc.relation.ispartofOsteoarthritis and Cartilageen_HK
dc.subject.meshAggrecansen_HK
dc.subject.meshAging - physiologyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCartilage, Articular - metabolismen_HK
dc.subject.meshCollagen - metabolismen_HK
dc.subject.meshCollagen Type II - metabolismen_HK
dc.subject.meshCollagen Type X - metabolismen_HK
dc.subject.meshCollagen Type XI - metabolismen_HK
dc.subject.meshExtracellular Matrix Proteinsen_HK
dc.subject.meshGene Expressionen_HK
dc.subject.meshIn Situ Hybridizationen_HK
dc.subject.meshLectins, C-Typeen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Inbred CBAen_HK
dc.subject.meshOsteoarthritis, Knee - metabolismen_HK
dc.subject.meshProteoglycans - metabolismen_HK
dc.titleExpression of collagen and aggrecan genes in normal and osteoarthritic murine knee jointsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1063-4584&volume=10&spage=51&epage=61&date=2002&atitle=Expression+of+collagen+and+aggrecan+genes+in+normal+and+osteoarthritic+murine+knee+jointsen_HK
dc.identifier.emailCheah, KSE:hrmbdkc@hku.hken_HK
dc.identifier.authorityCheah, KSE=rp00342en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1053/joca.2001.0481en_HK
dc.identifier.pmid11795983-
dc.identifier.scopuseid_2-s2.0-0036151195en_HK
dc.identifier.hkuros72858en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036151195&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume10en_HK
dc.identifier.issue1en_HK
dc.identifier.spage51en_HK
dc.identifier.epage61en_HK
dc.identifier.isiWOS:000173602600007-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridChambers, MG=7102115532en_HK
dc.identifier.scopusauthoridKuffner, T=6603038707en_HK
dc.identifier.scopusauthoridCowan, SK=7004660335en_HK
dc.identifier.scopusauthoridCheah, KSE=35387746200en_HK
dc.identifier.scopusauthoridMason, RM=7403491209en_HK

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