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Article: Hyaluronans: Crystallization-promoting activity and HPLC analysis of urinary excretion

TitleHyaluronans: Crystallization-promoting activity and HPLC analysis of urinary excretion
Authors
Issue Date1999
PublisherAmerican Society of Nephrology. The Journal's web site is located at http://www.jasn.org
Citation
Journal Of The American Society Of Nephrology, 1999, v. 10 SUPPL. 14, p. S397-S403 How to Cite?
AbstractThis work follows from our earlier report of a crystallization-promoting population in the leading electrophoretic fractions of pooled urinary glycosaminoglycan extracts of renal stone formers (GAG(SF)), but not in those of healthy individuals (GAG(H)). Preliminary HPLC analysis of disaccharide products of the fractions indicated the presence of chondroitin sulfates (CS) and hyaluronan (HA). Because both commercial CS and urinary CS of healthy individuals showed a basal crystallization-promoting property, we hypothesized that the observed property was due to HA excreted by SF and not by healthy individuals. Defined quantities of a commercial preparation of reference HA (pig skin, 40 to 60 kD) were first tested in urine ultrafiltrate (prepared at pH 5.3, 1250 mosmol/kg and nominal cutoff of 10 kD) by a freezing procedure to assess the crystallization-promoting property. The reference HA moderately promoted crystallization, yielding fewer crystals (5 to 10 times those in neat urine ultrafiltrate) than similar hexuronate concentrations of the HA-containing GAG(SF) population (25 to 30 times those in neat urine ultrafiltrate) reported earlier. Urinary GAG were then recovered individually from random urine samples of SF and healthy individuals. Products of sequential digestion by Streptomyces hyaluronidase and then chondroitinase ABC were analyzed by HPLC. Both GAG(SF) and GAG(H) indicated the presence of HA. Taken together, these results suggest that the crystallization-promoting property of HA(SF) does not generally apply to all HA preparations and that although healthy individuals also excrete HA, HA(H) does not share the property of HA(SF).
Persistent Identifierhttp://hdl.handle.net/10722/68026
ISSN
2015 Impact Factor: 8.491
2015 SCImago Journal Rankings: 4.699
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorShum, DKYen_HK
dc.contributor.authorGohel, MDIen_HK
dc.contributor.authorTam, PCen_HK
dc.date.accessioned2010-09-06T06:00:38Z-
dc.date.available2010-09-06T06:00:38Z-
dc.date.issued1999en_HK
dc.identifier.citationJournal Of The American Society Of Nephrology, 1999, v. 10 SUPPL. 14, p. S397-S403en_HK
dc.identifier.issn1046-6673en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68026-
dc.description.abstractThis work follows from our earlier report of a crystallization-promoting population in the leading electrophoretic fractions of pooled urinary glycosaminoglycan extracts of renal stone formers (GAG(SF)), but not in those of healthy individuals (GAG(H)). Preliminary HPLC analysis of disaccharide products of the fractions indicated the presence of chondroitin sulfates (CS) and hyaluronan (HA). Because both commercial CS and urinary CS of healthy individuals showed a basal crystallization-promoting property, we hypothesized that the observed property was due to HA excreted by SF and not by healthy individuals. Defined quantities of a commercial preparation of reference HA (pig skin, 40 to 60 kD) were first tested in urine ultrafiltrate (prepared at pH 5.3, 1250 mosmol/kg and nominal cutoff of 10 kD) by a freezing procedure to assess the crystallization-promoting property. The reference HA moderately promoted crystallization, yielding fewer crystals (5 to 10 times those in neat urine ultrafiltrate) than similar hexuronate concentrations of the HA-containing GAG(SF) population (25 to 30 times those in neat urine ultrafiltrate) reported earlier. Urinary GAG were then recovered individually from random urine samples of SF and healthy individuals. Products of sequential digestion by Streptomyces hyaluronidase and then chondroitinase ABC were analyzed by HPLC. Both GAG(SF) and GAG(H) indicated the presence of HA. Taken together, these results suggest that the crystallization-promoting property of HA(SF) does not generally apply to all HA preparations and that although healthy individuals also excrete HA, HA(H) does not share the property of HA(SF).en_HK
dc.languageengen_HK
dc.publisherAmerican Society of Nephrology. The Journal's web site is located at http://www.jasn.orgen_HK
dc.relation.ispartofJournal of the American Society of Nephrologyen_HK
dc.rightsJournal of the American Society of Nephrology. Copyright © Lippincott Williams & Wilkins.en_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshChromatography, High Pressure Liquiden_HK
dc.subject.meshCrystallizationen_HK
dc.subject.meshHumansen_HK
dc.subject.meshHyaluronic Acid - urineen_HK
dc.subject.meshKidney Calculi - etiologyen_HK
dc.subject.meshSwineen_HK
dc.titleHyaluronans: Crystallization-promoting activity and HPLC analysis of urinary excretionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1046-6673&volume=10&spage=S397&epage=S403&date=1999&atitle=Hyaluronans:+crystallization-promoting+activity+and+HPLC+analysis+of+urinary+excretionen_HK
dc.identifier.emailShum, DKY:shumdkhk@hkucc.hku.hken_HK
dc.identifier.authorityShum, DKY=rp00321en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid10541272-
dc.identifier.scopuseid_2-s2.0-0032724370en_HK
dc.identifier.hkuros47875en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032724370&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume10en_HK
dc.identifier.issueSUPPL. 14en_HK
dc.identifier.spageS397en_HK
dc.identifier.epageS403en_HK
dc.identifier.isiWOS:000083411800018-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridShum, DKY=7004824447en_HK
dc.identifier.scopusauthoridGohel, MDI=7004532182en_HK
dc.identifier.scopusauthoridTam, PC=7202539419en_HK

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