File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: A multi PDZ-domain protein Pdzd2 contributes to functional expression of sensory neuron-specific sodium channel NaV1.8

TitleA multi PDZ-domain protein Pdzd2 contributes to functional expression of sensory neuron-specific sodium channel NaV1.8
Authors
Issue Date2009
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ymcne
Citation
Molecular And Cellular Neuroscience, 2009, v. 42 n. 3, p. 219-225 How to Cite?
AbstractThe voltage-gated sodium channel NaV1.8 is expressed exclusively in nociceptive sensory neurons and plays an important role in pain pathways. NaV1.8 cannot be functionally expressed in non-neuronal cells even in the presence of β-subunits. We have previously identified Pdzd2, a multi PDZ-domain protein, as a potential interactor for NaV1.8. Here we report that Pdzd2 binds directly to the intracellular loops of NaV1.8 and NaV1.7. The endogenous NaV1.8 current in sensory neurons is inhibited by antisense- and siRNA-mediated downregulation of Pdzd2. However, no marked change in pain behaviours is observed in Pdzd2-decificent mice. This may be due to compensatory upregulation of p11, another regulatory factor for NaV1.8, in dorsal root ganglia of Pdzd2-deficient mice. These findings reveal that Pdzd2 and p11 play collaborative roles in regulation of NaV1.8 expression in sensory neurons. © 2009 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/68025
ISSN
2015 Impact Factor: 3.597
2015 SCImago Journal Rankings: 2.342
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Wellcome Trust
HKSAR, China
University Grants Committee of the University of HongHKU 7474/04M
AoE/M-04/04
Funding Information:

We thank the Wellcome Trust (DS, MB, WP, JW, KO) and Research Grants Council, HKSAR, China and the University Grants Committee of the University of Hong (KMY: HKU 7474/04M, KC: AoE/M-04/04) for their generous support. We also thank Y. Hata (Tokyo Medical and Dental University) for pCIneo-Papin.

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorShao, Den_HK
dc.contributor.authorBaker, MDen_HK
dc.contributor.authorAbrahamsen, Ben_HK
dc.contributor.authorRugiero, Fen_HK
dc.contributor.authorMalikHall, Men_HK
dc.contributor.authorPoon, WYLen_HK
dc.contributor.authorCheah, KSEen_HK
dc.contributor.authorYao, KMen_HK
dc.contributor.authorWood, JNen_HK
dc.contributor.authorOkuse, Ken_HK
dc.date.accessioned2010-09-06T06:00:37Z-
dc.date.available2010-09-06T06:00:37Z-
dc.date.issued2009en_HK
dc.identifier.citationMolecular And Cellular Neuroscience, 2009, v. 42 n. 3, p. 219-225en_HK
dc.identifier.issn1044-7431en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68025-
dc.description.abstractThe voltage-gated sodium channel NaV1.8 is expressed exclusively in nociceptive sensory neurons and plays an important role in pain pathways. NaV1.8 cannot be functionally expressed in non-neuronal cells even in the presence of β-subunits. We have previously identified Pdzd2, a multi PDZ-domain protein, as a potential interactor for NaV1.8. Here we report that Pdzd2 binds directly to the intracellular loops of NaV1.8 and NaV1.7. The endogenous NaV1.8 current in sensory neurons is inhibited by antisense- and siRNA-mediated downregulation of Pdzd2. However, no marked change in pain behaviours is observed in Pdzd2-decificent mice. This may be due to compensatory upregulation of p11, another regulatory factor for NaV1.8, in dorsal root ganglia of Pdzd2-deficient mice. These findings reveal that Pdzd2 and p11 play collaborative roles in regulation of NaV1.8 expression in sensory neurons. © 2009 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ymcneen_HK
dc.relation.ispartofMolecular and Cellular Neuroscienceen_HK
dc.subject.meshAdaptor Proteins, Signal Transducing - genetics - metabolism-
dc.subject.meshNerve Tissue Proteins - genetics - metabolism-
dc.subject.meshPDZ Domains-
dc.subject.meshSensory Receptor Cells - cytology - metabolism-
dc.subject.meshSodium Channels - genetics - metabolism-
dc.titleA multi PDZ-domain protein Pdzd2 contributes to functional expression of sensory neuron-specific sodium channel NaV1.8en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1044-7431&volume=42&issue=3&spage=219&epage=225&date=2009&atitle=A+multi+PDZ-domain+protein+Pdzd2+contributes+to+functional+expression+of+sensory+neuron-specific+sodium+channel+Nav1.8en_HK
dc.identifier.emailCheah, KSE:hrmbdkc@hku.hken_HK
dc.identifier.emailYao, KM:kmyao@hku.hken_HK
dc.identifier.authorityCheah, KSE=rp00342en_HK
dc.identifier.authorityYao, KM=rp00344en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1016/j.mcn.2009.07.003en_HK
dc.identifier.pmid19607921-
dc.identifier.pmcidPMC2764382-
dc.identifier.scopuseid_2-s2.0-69249224518en_HK
dc.identifier.hkuros163791en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-69249224518&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume42en_HK
dc.identifier.issue3en_HK
dc.identifier.spage219en_HK
dc.identifier.epage225en_HK
dc.identifier.isiWOS:000273497600006-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectInvestigating the functional involvement of PDZD2 in the regulation of pancreatic beta cell gene expression and function-
dc.relation.projectDevelopmental genomics and skeletal research-
dc.identifier.scopusauthoridShao, D=7006765448en_HK
dc.identifier.scopusauthoridBaker, MD=34567991000en_HK
dc.identifier.scopusauthoridAbrahamsen, B=7003987415en_HK
dc.identifier.scopusauthoridRugiero, F=12766057300en_HK
dc.identifier.scopusauthoridMalikHall, M=6507366190en_HK
dc.identifier.scopusauthoridPoon, WYL=7103025533en_HK
dc.identifier.scopusauthoridCheah, KSE=35387746200en_HK
dc.identifier.scopusauthoridYao, KM=7403234578en_HK
dc.identifier.scopusauthoridWood, JN=7404353149en_HK
dc.identifier.scopusauthoridOkuse, K=6603069995en_HK
dc.identifier.citeulike5337443-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats