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Article: Melatonin attenuates rat carotid chemoreceptor response to hypercapnic acidosis

TitleMelatonin attenuates rat carotid chemoreceptor response to hypercapnic acidosis
Authors
KeywordsCarotid chemoreceptor
Circadian
Hypercapnia
Iype I cells
Melatonin
Melatonin receptors
pH
Issue Date2004
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JPI
Citation
Journal Of Pineal Research, 2004, v. 36 n. 1, p. 49-57 How to Cite?
AbstractRespiratory activity is under circadian modulation and the physiological mechanisms may involve the pineal secretory product, melatonin, and the carotid chemoreceptor. We hypothesized that melatonin modulates the carotid chemoreceptor response to hypercapnic acidosis. To determine whether the effect of melatonin on the chemoreceptor response to hypercapnic acidosis is mediated by melatonin receptors in the chemosensitive cells, cytosolic calcium ([Ca 2+]i) was measured by spectrofluorometry in fura-2-loaded glomus cells dissociated from rat carotid bodies. Melatonin (0.01-10 nM) per se did not change the [Ca2+]i levels of the glomus cells but it concentration-dependently attenuated the peak [Ca2+]i response to hypercapnic acidosis in the glomus cells. In addition, the [Ca 2+]i response was attenuated by 2-iodomelatonin, an agonist of melatonin receptors. The melatonin-induced attenuation of the [Ca2+]i response to hypercapnic acidosis was abolished by pretreatment with an non-selective mt1/MT2 antagonist, luzindole, and by MT2 antagonists, 4-phenyl-2-propionamidotetraline or DH97. In situ hybridization study with antisense mt1 and MT 2 receptor mRNA oligonucleotide probes showed an expression of mt1 and MT2 receptors in the rat carotid body. Also, melatonin attenuated the carotid afferent response to hypercapnic acidosis in single- or pauci-fibers recorded from the sinus nerve in isolated carotid bodies superfused with bicarbonate-buffer saline. Results suggest that an activation of the melatonin receptors expressed in the glomus cells of the rat carotid body reduces the chemoreceptor response to hypercapnic acidosis. This modulation may play a physiological role in the influence of the circadian rhythms on the chemoreflex.
Persistent Identifierhttp://hdl.handle.net/10722/67979
ISSN
2015 Impact Factor: 9.314
2015 SCImago Journal Rankings: 2.655
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTjong, YWen_HK
dc.contributor.authorChen, Yen_HK
dc.contributor.authorLiong, ECen_HK
dc.contributor.authorIp, SFen_HK
dc.contributor.authorTipoe, GLen_HK
dc.contributor.authorFung, MLen_HK
dc.date.accessioned2010-09-06T06:00:01Z-
dc.date.available2010-09-06T06:00:01Z-
dc.date.issued2004en_HK
dc.identifier.citationJournal Of Pineal Research, 2004, v. 36 n. 1, p. 49-57en_HK
dc.identifier.issn0742-3098en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67979-
dc.description.abstractRespiratory activity is under circadian modulation and the physiological mechanisms may involve the pineal secretory product, melatonin, and the carotid chemoreceptor. We hypothesized that melatonin modulates the carotid chemoreceptor response to hypercapnic acidosis. To determine whether the effect of melatonin on the chemoreceptor response to hypercapnic acidosis is mediated by melatonin receptors in the chemosensitive cells, cytosolic calcium ([Ca 2+]i) was measured by spectrofluorometry in fura-2-loaded glomus cells dissociated from rat carotid bodies. Melatonin (0.01-10 nM) per se did not change the [Ca2+]i levels of the glomus cells but it concentration-dependently attenuated the peak [Ca2+]i response to hypercapnic acidosis in the glomus cells. In addition, the [Ca 2+]i response was attenuated by 2-iodomelatonin, an agonist of melatonin receptors. The melatonin-induced attenuation of the [Ca2+]i response to hypercapnic acidosis was abolished by pretreatment with an non-selective mt1/MT2 antagonist, luzindole, and by MT2 antagonists, 4-phenyl-2-propionamidotetraline or DH97. In situ hybridization study with antisense mt1 and MT 2 receptor mRNA oligonucleotide probes showed an expression of mt1 and MT2 receptors in the rat carotid body. Also, melatonin attenuated the carotid afferent response to hypercapnic acidosis in single- or pauci-fibers recorded from the sinus nerve in isolated carotid bodies superfused with bicarbonate-buffer saline. Results suggest that an activation of the melatonin receptors expressed in the glomus cells of the rat carotid body reduces the chemoreceptor response to hypercapnic acidosis. This modulation may play a physiological role in the influence of the circadian rhythms on the chemoreflex.en_HK
dc.languageengen_HK
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JPIen_HK
dc.relation.ispartofJournal of Pineal Researchen_HK
dc.subjectCarotid chemoreceptoren_HK
dc.subjectCircadianen_HK
dc.subjectHypercapniaen_HK
dc.subjectIype I cellsen_HK
dc.subjectMelatoninen_HK
dc.subjectMelatonin receptorsen_HK
dc.subjectpHen_HK
dc.subject.meshAcidosis, Respiratory - drug therapy - metabolismen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCalcium - metabolismen_HK
dc.subject.meshCarotid Body - cytology - drug effects - metabolismen_HK
dc.subject.meshChemoreceptor Cells - drug effectsen_HK
dc.subject.meshElectrophysiology - methodsen_HK
dc.subject.meshFura-2 - metabolismen_HK
dc.subject.meshHypercapnia - drug therapy - metabolismen_HK
dc.subject.meshMelatonin - analogs & derivatives - antagonists & inhibitors - pharmacology - physiologyen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.subject.meshReceptor, Melatonin, MT1 - antagonists & inhibitors - genetics - metabolismen_HK
dc.subject.meshReceptor, Melatonin, MT2 - antagonists & inhibitors - genetics - metabolismen_HK
dc.subject.meshReceptors, Melatonin - antagonists & inhibitors - drug effectsen_HK
dc.subject.meshTetrahydronaphthalenes - pharmacologyen_HK
dc.subject.meshTryptamines - pharmacologyen_HK
dc.titleMelatonin attenuates rat carotid chemoreceptor response to hypercapnic acidosisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0742-3098&volume=36&spage=49&epage=57&date=2004&atitle=Melatonin+attenuates+rat+carotid+chemoreceptor+response+to+hypercapnic+acidosisen_HK
dc.identifier.emailTipoe, GL: tgeorge@hkucc.hku.hken_HK
dc.identifier.emailFung, ML: fungml@hkucc.hku.hken_HK
dc.identifier.authorityTipoe, GL=rp00371en_HK
dc.identifier.authorityFung, ML=rp00433en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1046/j.1600-079X.2003.00094.xen_HK
dc.identifier.pmid14675130-
dc.identifier.scopuseid_2-s2.0-1642521863en_HK
dc.identifier.hkuros85490en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-1642521863&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume36en_HK
dc.identifier.issue1en_HK
dc.identifier.spage49en_HK
dc.identifier.epage57en_HK
dc.identifier.isiWOS:000187275900007-
dc.publisher.placeDenmarken_HK
dc.identifier.scopusauthoridTjong, YW=6507176524en_HK
dc.identifier.scopusauthoridChen, Y=7601439932en_HK
dc.identifier.scopusauthoridLiong, EC=6602732210en_HK
dc.identifier.scopusauthoridIp, SF=8595610100en_HK
dc.identifier.scopusauthoridTipoe, GL=7003550610en_HK
dc.identifier.scopusauthoridFung, ML=7101955092en_HK

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