File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Influence of peripheral nerve grafts on the expression of GAP-43 in regenerating retinal ganglion cells in adult hamsters

TitleInfluence of peripheral nerve grafts on the expression of GAP-43 in regenerating retinal ganglion cells in adult hamsters
Authors
Issue Date1995
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0300-4864
Citation
Journal Of Neurocytology, 1995, v. 24 n. 7, p. 487-496 How to Cite?
AbstractWe have examined the ability of axotomized retinal ganglion cells in adult hamsters, to regenerate axons into a peripheral nerve graft attached to the optic nerve and the expression of GAP-43 by these neurons. We also examined the effect on these events of transplanting a segment of peripheral nerve to the vitreous body. The left optic nerves in three groups of hamsters were replaced with a long segment of peripheral nerve attached to the proximal stump of the optic nerve approximately 2 mm from the optic disc to induce regeneration of retinal ganglion cells into the peripheral nerve. An additional segment of peripheral nerve was transplanted into the vitreous of the left eye in the second group. The animals from the first and second groups were allowed to survive for 1-8 weeks and the number of regenerating retinal ganglion cells was determined by applying the retrograde tracer, Fluoro-Gold to the peripheral nerve graft and the expression of GAP-43 was studied by immunocytochemistry in the same retinas. As a control, a segment of optic nerve was transplanted into the vitreous body of the left eye in the third group of hamsters. These animals were allowed to survive for 4 weeks and the number of regenerating retinal ganglion cells was counted as in Groups 1 and 2. The percentages of the regenerating retinal ganglion cells which also expressed GAP-43 were very high at all time points in Group 1 (with no intravitreal peripheral nerve) and Group 2 (with intravitreal peripheral nerve) and at 4 weeks for the Group 3 (with intravitreal optic nerve) animals. In addition, the number of regenerating retinal ganglion cells, the number of retinal ganglion cells expressing GAP-43 and the number of regenerating retinal ganglion cells which also expressed GAP-43 were much higher in Group 2 than in Group 3 at all the time points and it was also much higher in Group 2 than in Group 3 at 4 weeks whereas there was no significant difference between the results from Groups 1 and 3 at 4 weeks. These data suggested that there was a close correlation between the number of the axotomized retinal ganglion cells regenerating axons into the peripheral nerve graft attached to the optic nerve and the expression of GAP-43. In addition, the intravitreal peripheral nerve, probably by releasing various neurotrophic factors and by acting synergistically, can enhance the expression of GAP-43 in some of the axotomized retinal ganglion cells and promote the regeneration of retinal ganglion cells into the peripheral nerve graft.
Persistent Identifierhttp://hdl.handle.net/10722/67971
ISSN
2007 Impact Factor: 1.935
2009 SCImago Journal Rankings: 0.911
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorNg, TFen_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorChung, SKen_HK
dc.date.accessioned2010-09-06T05:59:56Z-
dc.date.available2010-09-06T05:59:56Z-
dc.date.issued1995en_HK
dc.identifier.citationJournal Of Neurocytology, 1995, v. 24 n. 7, p. 487-496en_HK
dc.identifier.issn0300-4864en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67971-
dc.description.abstractWe have examined the ability of axotomized retinal ganglion cells in adult hamsters, to regenerate axons into a peripheral nerve graft attached to the optic nerve and the expression of GAP-43 by these neurons. We also examined the effect on these events of transplanting a segment of peripheral nerve to the vitreous body. The left optic nerves in three groups of hamsters were replaced with a long segment of peripheral nerve attached to the proximal stump of the optic nerve approximately 2 mm from the optic disc to induce regeneration of retinal ganglion cells into the peripheral nerve. An additional segment of peripheral nerve was transplanted into the vitreous of the left eye in the second group. The animals from the first and second groups were allowed to survive for 1-8 weeks and the number of regenerating retinal ganglion cells was determined by applying the retrograde tracer, Fluoro-Gold to the peripheral nerve graft and the expression of GAP-43 was studied by immunocytochemistry in the same retinas. As a control, a segment of optic nerve was transplanted into the vitreous body of the left eye in the third group of hamsters. These animals were allowed to survive for 4 weeks and the number of regenerating retinal ganglion cells was counted as in Groups 1 and 2. The percentages of the regenerating retinal ganglion cells which also expressed GAP-43 were very high at all time points in Group 1 (with no intravitreal peripheral nerve) and Group 2 (with intravitreal peripheral nerve) and at 4 weeks for the Group 3 (with intravitreal optic nerve) animals. In addition, the number of regenerating retinal ganglion cells, the number of retinal ganglion cells expressing GAP-43 and the number of regenerating retinal ganglion cells which also expressed GAP-43 were much higher in Group 2 than in Group 3 at all the time points and it was also much higher in Group 2 than in Group 3 at 4 weeks whereas there was no significant difference between the results from Groups 1 and 3 at 4 weeks. These data suggested that there was a close correlation between the number of the axotomized retinal ganglion cells regenerating axons into the peripheral nerve graft attached to the optic nerve and the expression of GAP-43. In addition, the intravitreal peripheral nerve, probably by releasing various neurotrophic factors and by acting synergistically, can enhance the expression of GAP-43 in some of the axotomized retinal ganglion cells and promote the regeneration of retinal ganglion cells into the peripheral nerve graft.en_HK
dc.languageengen_HK
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0300-4864en_HK
dc.relation.ispartofJournal of Neurocytologyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAxons - physiologyen_HK
dc.subject.meshCricetinaeen_HK
dc.subject.meshGAP-43 Proteinen_HK
dc.subject.meshGene Expressionen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMembrane Glycoproteins - biosynthesisen_HK
dc.subject.meshMesocricetusen_HK
dc.subject.meshNerve Regenerationen_HK
dc.subject.meshNerve Tissue Proteins - biosynthesisen_HK
dc.subject.meshNeurofilament Proteins - biosynthesisen_HK
dc.subject.meshOptic Nerve - cytology - physiologyen_HK
dc.subject.meshPeroneal Nerve - physiology - transplantationen_HK
dc.subject.meshRetinal Ganglion Cells - cytology - metabolism - physiologyen_HK
dc.subject.meshVitreous Bodyen_HK
dc.titleInfluence of peripheral nerve grafts on the expression of GAP-43 in regenerating retinal ganglion cells in adult hamstersen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0300-4864&volume=24&spage=487&epage=496&date=1995&atitle=Influence+of+peripheral+nerve+grafts+on+the+expression+of+GAP-43+in+regenerating+retinal+ganglion+cells+in+adult+hamstersen_HK
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_HK
dc.identifier.emailChung, SK:skchung@hkucc.hku.hken_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.identifier.authorityChung, SK=rp00381en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/BF01179974en_HK
dc.identifier.pmid7561957-
dc.identifier.scopuseid_2-s2.0-0029143476en_HK
dc.identifier.hkuros8488en_HK
dc.identifier.volume24en_HK
dc.identifier.issue7en_HK
dc.identifier.spage487en_HK
dc.identifier.epage496en_HK
dc.identifier.isiWOS:A1995RM21900001-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridNg, TF=36799699400en_HK
dc.identifier.scopusauthoridSo, KF=34668391300en_HK
dc.identifier.scopusauthoridChung, SK=7404292976en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats