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Article: Identification of downstream target genes of latent membrane protein 1 in nasopharyngeal carcinoma cells by suppression subtractive hybridization
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TitleIdentification of downstream target genes of latent membrane protein 1 in nasopharyngeal carcinoma cells by suppression subtractive hybridization
 
AuthorsLo, AKF1
Liu, Y1
Wang, X1
Yong Chuan Wong1
Lee, CKF1
Huang, DP1
Sai Wah Tsao1
 
KeywordsLatent membrane protein 1
Nasopharyngeal carcinoma
Suppression subtractive hybridization
 
Issue Date2001
 
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbaexp
 
CitationBiochimica Et Biophysica Acta - Gene Structure And Expression, 2001, v. 1520 n. 2, p. 131-140 [How to Cite?]
DOI: http://dx.doi.org/10.1016/S0167-4781(01)00260-3
 
AbstractNasopharyngeal carcinoma (NPC) is a common cancer in Southern China and is closely associated with infection of Epstein-Barr virus (EBV). The EBV encoded latent membrane protein 1 (LMP1) is frequently detected in NPC and may play a role in its pathogenesis. Previous studies have shown that LMP1 transformed rodent fibroblasts and altered growth properties in B cells and epithelial cells. However, the pathological role of LMP1 in NPC cells is still poorly understood. In order to investigate the downstream target genes of LMP1 in NPC cells, suppression subtractive hybridization was used to clone and identify the genes differentially expressed in a LMP1 expressing NPC cell line, CNE-2. Two subtractive cDNA libraries were constructed: one enriched for the genes upregulated by LMP1 and one was for the genes downregulated by LMP1. A total of 192 clones were screened by reverse Northern blotting. Fourteen of them were confirmed to be overexpressed while eight of them were suppressed. The upregulation of integrin α6, laminin 5γ2, TAP1 and downregulation of p54nrb, RACK1 and p66Shc were further confirmed in three sets of LMP1 expressing NPC cell lines. The expression profiles of differentially expressed genes identified in this study suggest a role of LMP1 in promotion of cell survival and facilitation of tumor invasion. © 2001 Elsevier Science B.V. All rights reserved.
 
ISSN0167-4781
 
DOIhttp://dx.doi.org/10.1016/S0167-4781(01)00260-3
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorLo, AKF
 
dc.contributor.authorLiu, Y
 
dc.contributor.authorWang, X
 
dc.contributor.authorYong Chuan Wong
 
dc.contributor.authorLee, CKF
 
dc.contributor.authorHuang, DP
 
dc.contributor.authorSai Wah Tsao
 
dc.date.accessioned2010-09-06T05:59:50Z
 
dc.date.available2010-09-06T05:59:50Z
 
dc.date.issued2001
 
dc.description.abstractNasopharyngeal carcinoma (NPC) is a common cancer in Southern China and is closely associated with infection of Epstein-Barr virus (EBV). The EBV encoded latent membrane protein 1 (LMP1) is frequently detected in NPC and may play a role in its pathogenesis. Previous studies have shown that LMP1 transformed rodent fibroblasts and altered growth properties in B cells and epithelial cells. However, the pathological role of LMP1 in NPC cells is still poorly understood. In order to investigate the downstream target genes of LMP1 in NPC cells, suppression subtractive hybridization was used to clone and identify the genes differentially expressed in a LMP1 expressing NPC cell line, CNE-2. Two subtractive cDNA libraries were constructed: one enriched for the genes upregulated by LMP1 and one was for the genes downregulated by LMP1. A total of 192 clones were screened by reverse Northern blotting. Fourteen of them were confirmed to be overexpressed while eight of them were suppressed. The upregulation of integrin α6, laminin 5γ2, TAP1 and downregulation of p54nrb, RACK1 and p66Shc were further confirmed in three sets of LMP1 expressing NPC cell lines. The expression profiles of differentially expressed genes identified in this study suggest a role of LMP1 in promotion of cell survival and facilitation of tumor invasion. © 2001 Elsevier Science B.V. All rights reserved.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationBiochimica Et Biophysica Acta - Gene Structure And Expression, 2001, v. 1520 n. 2, p. 131-140 [How to Cite?]
DOI: http://dx.doi.org/10.1016/S0167-4781(01)00260-3
 
dc.identifier.doihttp://dx.doi.org/10.1016/S0167-4781(01)00260-3
 
dc.identifier.epage140
 
dc.identifier.hkuros64369
 
dc.identifier.issn0167-4781
 
dc.identifier.issue2
 
dc.identifier.openurl
 
dc.identifier.pmid11513954
 
dc.identifier.scopuseid_2-s2.0-0035975112
 
dc.identifier.spage131
 
dc.identifier.urihttp://hdl.handle.net/10722/67960
 
dc.identifier.volume1520
 
dc.languageeng
 
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbaexp
 
dc.publisher.placeNetherlands
 
dc.relation.ispartofBiochimica et Biophysica Acta - Gene Structure and Expression
 
dc.relation.referencesReferences in Scopus
 
dc.rightsBiochimica et Biophysica Acta. Copyright © Elsevier BV.
 
dc.subject.meshDNA, Complementary - biosynthesis - chemistry
 
dc.subject.meshGene Library
 
dc.subject.meshHumans
 
dc.subject.meshNasopharyngeal Neoplasms - genetics
 
dc.subject.meshNeoplasm Invasiveness
 
dc.subject.meshNeoplasm Proteins - biosynthesis - genetics
 
dc.subject.meshNucleic Acid Hybridization - methods
 
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction
 
dc.subject.meshSequence Homology, Nucleic Acid
 
dc.subject.meshSignal Transduction
 
dc.subject.meshTumor Cells, Cultured
 
dc.subject.meshViral Matrix Proteins - biosynthesis - genetics
 
dc.subjectLatent membrane protein 1
 
dc.subjectNasopharyngeal carcinoma
 
dc.subjectSuppression subtractive hybridization
 
dc.titleIdentification of downstream target genes of latent membrane protein 1 in nasopharyngeal carcinoma cells by suppression subtractive hybridization
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong