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Article: Identification of downstream target genes of latent membrane protein 1 in nasopharyngeal carcinoma cells by suppression subtractive hybridization

TitleIdentification of downstream target genes of latent membrane protein 1 in nasopharyngeal carcinoma cells by suppression subtractive hybridization
Authors
KeywordsLatent membrane protein 1
Nasopharyngeal carcinoma
Suppression subtractive hybridization
Issue Date2001
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbaexp
Citation
Biochimica Et Biophysica Acta - Gene Structure And Expression, 2001, v. 1520 n. 2, p. 131-140 How to Cite?
Abstract
Nasopharyngeal carcinoma (NPC) is a common cancer in Southern China and is closely associated with infection of Epstein-Barr virus (EBV). The EBV encoded latent membrane protein 1 (LMP1) is frequently detected in NPC and may play a role in its pathogenesis. Previous studies have shown that LMP1 transformed rodent fibroblasts and altered growth properties in B cells and epithelial cells. However, the pathological role of LMP1 in NPC cells is still poorly understood. In order to investigate the downstream target genes of LMP1 in NPC cells, suppression subtractive hybridization was used to clone and identify the genes differentially expressed in a LMP1 expressing NPC cell line, CNE-2. Two subtractive cDNA libraries were constructed: one enriched for the genes upregulated by LMP1 and one was for the genes downregulated by LMP1. A total of 192 clones were screened by reverse Northern blotting. Fourteen of them were confirmed to be overexpressed while eight of them were suppressed. The upregulation of integrin α6, laminin 5γ2, TAP1 and downregulation of p54nrb, RACK1 and p66Shc were further confirmed in three sets of LMP1 expressing NPC cell lines. The expression profiles of differentially expressed genes identified in this study suggest a role of LMP1 in promotion of cell survival and facilitation of tumor invasion. © 2001 Elsevier Science B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/67960
ISSN
References

 

Author Affiliations
  1. The University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorLo, AKFen_HK
dc.contributor.authorLiu, Yen_HK
dc.contributor.authorWang, Xen_HK
dc.contributor.authorYong Chuan Wongen_HK
dc.contributor.authorLee, CKFen_HK
dc.contributor.authorHuang, DPen_HK
dc.contributor.authorSai Wah Tsaoen_HK
dc.date.accessioned2010-09-06T05:59:50Z-
dc.date.available2010-09-06T05:59:50Z-
dc.date.issued2001en_HK
dc.identifier.citationBiochimica Et Biophysica Acta - Gene Structure And Expression, 2001, v. 1520 n. 2, p. 131-140en_HK
dc.identifier.issn0167-4781en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67960-
dc.description.abstractNasopharyngeal carcinoma (NPC) is a common cancer in Southern China and is closely associated with infection of Epstein-Barr virus (EBV). The EBV encoded latent membrane protein 1 (LMP1) is frequently detected in NPC and may play a role in its pathogenesis. Previous studies have shown that LMP1 transformed rodent fibroblasts and altered growth properties in B cells and epithelial cells. However, the pathological role of LMP1 in NPC cells is still poorly understood. In order to investigate the downstream target genes of LMP1 in NPC cells, suppression subtractive hybridization was used to clone and identify the genes differentially expressed in a LMP1 expressing NPC cell line, CNE-2. Two subtractive cDNA libraries were constructed: one enriched for the genes upregulated by LMP1 and one was for the genes downregulated by LMP1. A total of 192 clones were screened by reverse Northern blotting. Fourteen of them were confirmed to be overexpressed while eight of them were suppressed. The upregulation of integrin α6, laminin 5γ2, TAP1 and downregulation of p54nrb, RACK1 and p66Shc were further confirmed in three sets of LMP1 expressing NPC cell lines. The expression profiles of differentially expressed genes identified in this study suggest a role of LMP1 in promotion of cell survival and facilitation of tumor invasion. © 2001 Elsevier Science B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbaexpen_HK
dc.relation.ispartofBiochimica et Biophysica Acta - Gene Structure and Expressionen_HK
dc.rightsBiochimica et Biophysica Acta. Copyright © Elsevier BV.en_HK
dc.subjectLatent membrane protein 1en_HK
dc.subjectNasopharyngeal carcinomaen_HK
dc.subjectSuppression subtractive hybridizationen_HK
dc.subject.meshDNA, Complementary - biosynthesis - chemistryen_HK
dc.subject.meshGene Libraryen_HK
dc.subject.meshHumansen_HK
dc.subject.meshNasopharyngeal Neoplasms - geneticsen_HK
dc.subject.meshNeoplasm Invasivenessen_HK
dc.subject.meshNeoplasm Proteins - biosynthesis - geneticsen_HK
dc.subject.meshNucleic Acid Hybridization - methodsen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshSequence Homology, Nucleic Aciden_HK
dc.subject.meshSignal Transductionen_HK
dc.subject.meshTumor Cells, Cultureden_HK
dc.subject.meshViral Matrix Proteins - biosynthesis - geneticsen_HK
dc.titleIdentification of downstream target genes of latent membrane protein 1 in nasopharyngeal carcinoma cells by suppression subtractive hybridizationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-3002&volume=1520&spage=131&epage=140&date=2001&atitle=Identification+of+downstream+target+genes+of+latent+membrane+protein+1+in+nasopharyngeal+carcinoma+cells+by+suppression+subtractive+hybridizationen_HK
dc.identifier.emailLee, CKF:ckflee@hku.hken_HK
dc.identifier.emailSai Wah Tsao:gswtsao@hkucc.hku.hken_HK
dc.identifier.authorityLee, CKF=rp00458en_HK
dc.identifier.authoritySai Wah Tsao=rp00399en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0167-4781(01)00260-3en_HK
dc.identifier.pmid11513954en_HK
dc.identifier.scopuseid_2-s2.0-0035975112en_HK
dc.identifier.hkuros64369en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035975112&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1520en_HK
dc.identifier.issue2en_HK
dc.identifier.spage131en_HK
dc.identifier.epage140en_HK
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLo, AKF=7102780657en_HK
dc.identifier.scopusauthoridLiu, Y=26643293600en_HK
dc.identifier.scopusauthoridWang, X=23053054900en_HK
dc.identifier.scopusauthoridYong Chuan Wong=23053591000en_HK
dc.identifier.scopusauthoridLee, CKF=26643097500en_HK
dc.identifier.scopusauthoridHuang, DP=7403891486en_HK
dc.identifier.scopusauthoridSai Wah Tsao=7102813116en_HK

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