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Article: Fabrication of nano-fibrous collagen microspheres for protein delivery and effects of photochemical crosslinking on release kinetics

TitleFabrication of nano-fibrous collagen microspheres for protein delivery and effects of photochemical crosslinking on release kinetics
Authors
KeywordsCollagen microspheres
Controlled release
Nano-fibrous
Photochemical crosslinking
Protein delivery
Issue Date2008
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jconrel
Citation
Journal Of Controlled Release, 2008, v. 129 n. 2, p. 135-143 How to Cite?
AbstractProtein compatibility is important for protein drug delivery using microsphere-based devices. Collagen has excellent protein compatibility but has poor mechanical stability for microsphere fabrication and open meshwork for controlled release. In this study, a protein-compatible fabrication method for injectable collagen microspheres has been developed. The surface morphology, interior microstructure and protein release characteristics of collagen microspheres were investigated. Moreover, effects of photochemical crosslinking on these characteristics were also studied. Finally, the mechanisms governing the protein release and the retention of protein bioactivity were studied. Stable and injectable collagen microspheres consisting of nano-fibrous meshwork were successfully fabricated under ambient conditions in an organic solvent and crosslinking reagent-free manner. These microspheres have open meshwork and showed large initial burst and rapid release of proteins. Photochemical crosslinking significantly reduced the initial burst effect and controlled the protein release in a photosensitizer dose-dependent manner without significantly altering the mesh size. We further demonstrated that there was significantly higher protein retention within the photochemically crosslinked collagen microspheres as compared with the uncrosslinked, suggesting a secondary retention mechanism. Lastly, both surfactant treatment and photochemical crosslinking did not compromise the bioactivity of the encapsulated proteins. In summary, this study reports a novel collagen microsphere-based protein delivery system and demonstrates the possibility to use photochemical crosslinking as the secondary retention mechanism for proteins. © 2008 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/67941
ISSN
2015 Impact Factor: 7.441
2015 SCImago Journal Rankings: 2.827
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, OCMen_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorChan, BPen_HK
dc.date.accessioned2010-09-06T05:59:39Z-
dc.date.available2010-09-06T05:59:39Z-
dc.date.issued2008en_HK
dc.identifier.citationJournal Of Controlled Release, 2008, v. 129 n. 2, p. 135-143en_HK
dc.identifier.issn0168-3659en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67941-
dc.description.abstractProtein compatibility is important for protein drug delivery using microsphere-based devices. Collagen has excellent protein compatibility but has poor mechanical stability for microsphere fabrication and open meshwork for controlled release. In this study, a protein-compatible fabrication method for injectable collagen microspheres has been developed. The surface morphology, interior microstructure and protein release characteristics of collagen microspheres were investigated. Moreover, effects of photochemical crosslinking on these characteristics were also studied. Finally, the mechanisms governing the protein release and the retention of protein bioactivity were studied. Stable and injectable collagen microspheres consisting of nano-fibrous meshwork were successfully fabricated under ambient conditions in an organic solvent and crosslinking reagent-free manner. These microspheres have open meshwork and showed large initial burst and rapid release of proteins. Photochemical crosslinking significantly reduced the initial burst effect and controlled the protein release in a photosensitizer dose-dependent manner without significantly altering the mesh size. We further demonstrated that there was significantly higher protein retention within the photochemically crosslinked collagen microspheres as compared with the uncrosslinked, suggesting a secondary retention mechanism. Lastly, both surfactant treatment and photochemical crosslinking did not compromise the bioactivity of the encapsulated proteins. In summary, this study reports a novel collagen microsphere-based protein delivery system and demonstrates the possibility to use photochemical crosslinking as the secondary retention mechanism for proteins. © 2008 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jconrelen_HK
dc.relation.ispartofJournal of Controlled Releaseen_HK
dc.rightsJournal of Controlled Release. Copyright © Elsevier BV.en_HK
dc.subjectCollagen microspheresen_HK
dc.subjectControlled releaseen_HK
dc.subjectNano-fibrousen_HK
dc.subjectPhotochemical crosslinkingen_HK
dc.subjectProtein deliveryen_HK
dc.subject.meshChemistry, Pharmaceuticalen_HK
dc.subject.meshCollagen - chemistry - radiation effectsen_HK
dc.subject.meshDelayed-Action Preparationsen_HK
dc.subject.meshDrug Carriersen_HK
dc.subject.meshDrug Compoundingen_HK
dc.subject.meshKineticsen_HK
dc.subject.meshLasers, Gasen_HK
dc.subject.meshMicrospheresen_HK
dc.subject.meshNanostructuresen_HK
dc.subject.meshParticle Sizeen_HK
dc.subject.meshPhotochemistryen_HK
dc.subject.meshPolysorbates - chemistryen_HK
dc.subject.meshSerum Albumin, Bovine - chemistryen_HK
dc.subject.meshSolubilityen_HK
dc.subject.meshSurface Propertiesen_HK
dc.subject.meshSurface-Active Agents - chemistryen_HK
dc.subject.meshTechnology, Pharmaceutical - methodsen_HK
dc.titleFabrication of nano-fibrous collagen microspheres for protein delivery and effects of photochemical crosslinking on release kineticsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0168-3659&volume=129&spage=135&epage=143&date=2008&atitle=Fabrication+of+nano-fibrous+collagen+microspheres+for+protein+delivery+and+effects+of+photochemical+crosslinking+on+release+kineticsen_HK
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_HK
dc.identifier.emailChan, BP:bpchan@hkucc.hku.hken_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.identifier.authorityChan, BP=rp00087en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jconrel.2008.04.011en_HK
dc.identifier.pmid18514352en_HK
dc.identifier.scopuseid_2-s2.0-45249098472en_HK
dc.identifier.hkuros145980en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-45249098472&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume129en_HK
dc.identifier.issue2en_HK
dc.identifier.spage135en_HK
dc.identifier.epage143en_HK
dc.identifier.eissn1873-4995-
dc.identifier.isiWOS:000257959400011-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridChan, OCM=23049435400en_HK
dc.identifier.scopusauthoridSo, KF=34668391300en_HK
dc.identifier.scopusauthoridChan, BP=7201530390en_HK

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