File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Noninvasive monitoring of diabetes-induced cutaneous nerve fiber loss and hypoalgesia in thy1-YFP transgenic mice

TitleNoninvasive monitoring of diabetes-induced cutaneous nerve fiber loss and hypoalgesia in thy1-YFP transgenic mice
Authors
Issue Date2005
PublisherAmerican Diabetes Association. The Journal's web site is located at http://diabetes.diabetesjournals.org/
Citation
Diabetes, 2005, v. 54 n. 11, p. 3112-3118 How to Cite?
AbstractProgressive loss of pain perception and cutaneous nerve fibers are frequently observed in diabetic patients. We evaluated the feasibility of using thy1-YFP mice that express the yellowish-green fluorescent protein (YFP) in all of their sensory/motor neurons for noninvasive monitoring of cutaneous nerve fiber loss during diabetes. Fluorescent fibers in skin sections from the leg of thy1-YFP mice stained positive for the neuron-specific protein gene product 9.5 (PGP9.5), indicating that the cutaneous fluorescent fibers are indeed nerve fibers. In diabetic thy1-YFP mice, significant small cutaneous nerve fiber loss in the leg was observed at 3 months following the onset of diabetes, but loss of heat-induced pain perception occurred as early as 1 month following the onset of diabetes, indicating that functional impairment of sensory nerves precedes cutaneous nerve fiber loss. Immunostaining of skin sections of mice killed at 6 months following the onset of diabetes showed that parallel to the loss of small fluorescent nerve fibers, there was a significant decrease in fibers stained positive for calcitonin gene-related peptide, substance P, and purinoreceptor subtype in diabetic thy1-YFP mice. These mice will be useful for noninvasive monitoring of cutaneous nerve fiber degeneration and loss of heat-induced pain perception during diabetes and for the assessment of efficacy of therapeutic treatment of diabetic neuropathy. © 2005 by the American Diabetes Association.
Persistent Identifierhttp://hdl.handle.net/10722/67936
ISSN
2015 Impact Factor: 8.784
2015 SCImago Journal Rankings: 5.185
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYuk, SCen_HK
dc.contributor.authorChung, SSMen_HK
dc.contributor.authorChung, SKen_HK
dc.date.accessioned2010-09-06T05:59:37Z-
dc.date.available2010-09-06T05:59:37Z-
dc.date.issued2005en_HK
dc.identifier.citationDiabetes, 2005, v. 54 n. 11, p. 3112-3118en_HK
dc.identifier.issn0012-1797en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67936-
dc.description.abstractProgressive loss of pain perception and cutaneous nerve fibers are frequently observed in diabetic patients. We evaluated the feasibility of using thy1-YFP mice that express the yellowish-green fluorescent protein (YFP) in all of their sensory/motor neurons for noninvasive monitoring of cutaneous nerve fiber loss during diabetes. Fluorescent fibers in skin sections from the leg of thy1-YFP mice stained positive for the neuron-specific protein gene product 9.5 (PGP9.5), indicating that the cutaneous fluorescent fibers are indeed nerve fibers. In diabetic thy1-YFP mice, significant small cutaneous nerve fiber loss in the leg was observed at 3 months following the onset of diabetes, but loss of heat-induced pain perception occurred as early as 1 month following the onset of diabetes, indicating that functional impairment of sensory nerves precedes cutaneous nerve fiber loss. Immunostaining of skin sections of mice killed at 6 months following the onset of diabetes showed that parallel to the loss of small fluorescent nerve fibers, there was a significant decrease in fibers stained positive for calcitonin gene-related peptide, substance P, and purinoreceptor subtype in diabetic thy1-YFP mice. These mice will be useful for noninvasive monitoring of cutaneous nerve fiber degeneration and loss of heat-induced pain perception during diabetes and for the assessment of efficacy of therapeutic treatment of diabetic neuropathy. © 2005 by the American Diabetes Association.en_HK
dc.languageengen_HK
dc.publisherAmerican Diabetes Association. The Journal's web site is located at http://diabetes.diabetesjournals.org/en_HK
dc.relation.ispartofDiabetesen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAntigens, Thy-1 - geneticsen_HK
dc.subject.meshBacterial Proteins - analysis - geneticsen_HK
dc.subject.meshBlood Glucoseen_HK
dc.subject.meshDiabetes Mellitus, Experimental - complications - physiopathologyen_HK
dc.subject.meshGene Expression Regulationen_HK
dc.subject.meshGenes, Reporter - geneticsen_HK
dc.subject.meshLuminescent Proteins - analysis - geneticsen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Transgenicen_HK
dc.subject.meshNerve Fibers - pathologyen_HK
dc.subject.meshPain - physiopathologyen_HK
dc.subject.meshPromoter Regions, Genetic - geneticsen_HK
dc.subject.meshSkin - innervationen_HK
dc.subject.meshTime Factorsen_HK
dc.subject.meshUbiquitin Thiolesterase - metabolismen_HK
dc.titleNoninvasive monitoring of diabetes-induced cutaneous nerve fiber loss and hypoalgesia in thy1-YFP transgenic miceen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0012-1797&volume=54&issue=11&spage=3112&epage=3118&date=2005&atitle=Noninvasive+monitoring+of+diabetes-induced+cutaneous+nerve+fiber+loss+and+hypoalgesia+in+thy1-YFP+transgenic+miceen_HK
dc.identifier.emailChung, SSM: smchung@hkucc.hku.hken_HK
dc.identifier.emailChung, SK: skchung@hkucc.hku.hken_HK
dc.identifier.authorityChung, SSM=rp00376en_HK
dc.identifier.authorityChung, SK=rp00381en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.2337/diabetes.54.11.3112en_HK
dc.identifier.pmid16249433-
dc.identifier.scopuseid_2-s2.0-33644699967en_HK
dc.identifier.hkuros121111en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33644699967&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume54en_HK
dc.identifier.issue11en_HK
dc.identifier.spage3112en_HK
dc.identifier.epage3118en_HK
dc.identifier.isiWOS:000233023100007-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridYuk, SC=36922492300en_HK
dc.identifier.scopusauthoridChung, SSM=14120761600en_HK
dc.identifier.scopusauthoridChung, SK=7404292976en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats