File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Endothelial nitric oxide synthase is expressed in amacrine cells of developing human retinas

TitleEndothelial nitric oxide synthase is expressed in amacrine cells of developing human retinas
Authors
Issue Date2006
PublisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.org
Citation
Investigative Ophthalmology And Visual Science, 2006, v. 47 n. 5, p. 2141-2149 How to Cite?
AbstractPURPOSE. To examine the expression and cellular distribution pattern of endothelial nitric oxide synthase (eNOS) in the developing human retina and to compare its expression with that in rats. METHODS. Expression of eNOS was examined by immunohistochemistry in retinas of humans ranging from 8.5 to 28 weeks of gestation (WG) and of rats. RESULTS. In the developing human retina, eNOS expression was first detected in the proximal margin of the neuroblastic layer in the incipient fovea-surrounding area at 12 WG. At 17 to 28 WG, eNOS-immunoreactive cells were located in the innermost part of the inner nuclear layer and in the ganglion cell layer, expanding to both temporal and nasal retinas and the processes projecting into the inner plexiform layer. These eNOS-positive cells coexpressed syntaxin and glutamate decarboxylase, and are probably GABAergic amacrine cells. The onset of eNOS expression in developing amacrine cells, however, preceded the invasion of retinal vasculature, long before vascular function involving these cells can be expected, suggesting that eNOS has a role not only in vasoregulation but also in retinal development. From 20 WG on, eNOS was also detected in the photoreceptors adjacent to the fovea. eNOS expression in amacrine cells and photoreceptors was observed in the central-to-peripheral and temporal-to-nasal gradients. However, in the developing rat retina, eNOS was expressed exclusively in the vascular endothelial cells. CONCLUSIONS. The results support that eNOS plays a role, not only in the regulation of vascular function but also in the process of retinal development in humans. Copyright © Association for Research in Vision and Ophthalmology.
Persistent Identifierhttp://hdl.handle.net/10722/67933
ISSN
2015 Impact Factor: 3.427
2015 SCImago Journal Rankings: 2.008
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, Sen_HK
dc.contributor.authorTay, Den_HK
dc.contributor.authorShu, Sen_HK
dc.contributor.authorBao, Xen_HK
dc.contributor.authorWu, Yen_HK
dc.contributor.authorWang, Xen_HK
dc.contributor.authorYip, HKen_HK
dc.date.accessioned2010-09-06T05:59:35Z-
dc.date.available2010-09-06T05:59:35Z-
dc.date.issued2006en_HK
dc.identifier.citationInvestigative Ophthalmology And Visual Science, 2006, v. 47 n. 5, p. 2141-2149en_HK
dc.identifier.issn0146-0404en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67933-
dc.description.abstractPURPOSE. To examine the expression and cellular distribution pattern of endothelial nitric oxide synthase (eNOS) in the developing human retina and to compare its expression with that in rats. METHODS. Expression of eNOS was examined by immunohistochemistry in retinas of humans ranging from 8.5 to 28 weeks of gestation (WG) and of rats. RESULTS. In the developing human retina, eNOS expression was first detected in the proximal margin of the neuroblastic layer in the incipient fovea-surrounding area at 12 WG. At 17 to 28 WG, eNOS-immunoreactive cells were located in the innermost part of the inner nuclear layer and in the ganglion cell layer, expanding to both temporal and nasal retinas and the processes projecting into the inner plexiform layer. These eNOS-positive cells coexpressed syntaxin and glutamate decarboxylase, and are probably GABAergic amacrine cells. The onset of eNOS expression in developing amacrine cells, however, preceded the invasion of retinal vasculature, long before vascular function involving these cells can be expected, suggesting that eNOS has a role not only in vasoregulation but also in retinal development. From 20 WG on, eNOS was also detected in the photoreceptors adjacent to the fovea. eNOS expression in amacrine cells and photoreceptors was observed in the central-to-peripheral and temporal-to-nasal gradients. However, in the developing rat retina, eNOS was expressed exclusively in the vascular endothelial cells. CONCLUSIONS. The results support that eNOS plays a role, not only in the regulation of vascular function but also in the process of retinal development in humans. Copyright © Association for Research in Vision and Ophthalmology.en_HK
dc.languageengen_HK
dc.publisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.orgen_HK
dc.relation.ispartofInvestigative Ophthalmology and Visual Scienceen_HK
dc.subject.meshAmacrine Cells - embryology - enzymologyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshFluorescent Antibody Technique, Indirecten_HK
dc.subject.meshGestational Ageen_HK
dc.subject.meshGlutamate Decarboxylase - metabolismen_HK
dc.subject.meshHumansen_HK
dc.subject.meshKi-67 Antigen - metabolismen_HK
dc.subject.meshNitric Oxide Synthase Type III - metabolismen_HK
dc.subject.meshPhotoreceptor Cells, Vertebrate - enzymologyen_HK
dc.subject.meshQa-SNARE Proteins - metabolismen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.subject.meshRetina - embryologyen_HK
dc.titleEndothelial nitric oxide synthase is expressed in amacrine cells of developing human retinasen_HK
dc.typeArticleen_HK
dc.identifier.emailTay, D:dkctay@hkucc.hku.hken_HK
dc.identifier.emailYip, HK:hkfyip@hku.hken_HK
dc.identifier.authorityTay, D=rp00336en_HK
dc.identifier.authorityYip, HK=rp00285en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1167/iovs.04-1202en_HK
dc.identifier.pmid16639026en_HK
dc.identifier.scopuseid_2-s2.0-33744728644en_HK
dc.identifier.hkuros123129en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33744728644&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume47en_HK
dc.identifier.issue5en_HK
dc.identifier.spage2141en_HK
dc.identifier.epage2149en_HK
dc.identifier.isiWOS:000237451100055-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLi, S=23497101600en_HK
dc.identifier.scopusauthoridTay, D=7006796825en_HK
dc.identifier.scopusauthoridShu, S=16313893300en_HK
dc.identifier.scopusauthoridBao, X=7202850455en_HK
dc.identifier.scopusauthoridWu, Y=7406893313en_HK
dc.identifier.scopusauthoridWang, X=37084668400en_HK
dc.identifier.scopusauthoridYip, HK=7101980864en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats