File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Quantitative assessment of normal and potentially premalignant epithelium at different levels of human colorectal crypts

TitleQuantitative assessment of normal and potentially premalignant epithelium at different levels of human colorectal crypts
Authors
Issue Date1998
PublisherHistology and Histopathology. The Journal's web site is located at http://www.hh.um.es
Citation
Histology And Histopathology, 1998, v. 13 n. 2, p. 395-404 How to Cite?
AbstractThe present study uses morphometric techniques to assess whether altered differentiation patterns exist in PPM which might reflect its premalignant status. Samples were obtained from resected malignant lesions of large bowels of 10 Chinese patients. Normal (N) samples were biopsied from the margins of each resected large bowel. Potentially premalignant (PPM) mucosae were obtained from within 2 cm of the margins of the malignant lesions. Tissues were processed for histological examination and using strict criteria, colorectal crypts were divided into basal (B), intermediate (I) and surface (S) segments. Interactive digitisation of sections from each group was used to generate the following morphometric parameters in each segment: nuclear profile circularity indices (NSF and NCI); nuclear numerical density (N(A) and N(V)); the degree of deviation of the major nuclear axis in relation to the epithelial-connective junction (AGDMAX); cell height (CH); the distance between nuclear apex to cell apex (DNACA); the distance between cell base to nuclear apex (DCBNA); stratification index (SI) - the ratio of DCBNA and CH; and the volume density of mucous vacuoles in the reference epithelium (V(VMV,EP)). In comparisons of different segments within groups, the nuclei at the S segment of N and PPM crypts were more irregular and less circular in shape than nuclei from other segments. There was a shift of nuclear profile shape (NSF and NCI) from circular to ellipsiodal between B and S segments. In comparisons of similar segments between groups, no significant nuclear shape changes were detected in nuclei of PPM crypts when compared with nuclei in similar segments of N crypts and the pattern of nuclear shape alterations resembled those of normal crypts. In comparisons of different segments within groups of N and PPM crypts, AGDMAX. DNACA, DCBNA, CH and SI parameters demonstrated that epithelial cells at the I segments have more centrally positioned nuclei with the tallest epithelial height when compared with epithelial cells in other segments of both crypts. In B segments, nuclear N(A) and N(V) were almost double those of other segments in both N and PPM crypts, with marked reductions in these parameters between B and I segments. V(VMV,EP) was significantly highest in the I segment and significantly lowest in the S segment of both groups. Both N and PPM crypts showed similar trends in V(VMV,EP) within the crypt segments but when comparing similar segments between both crypts, a significant difference was detected only between S segments. The alterations of nuclear shape and packing densities, orientation and mucous content in N crypts were similarly expressed in PPM crypts and distinct differences in numerical density (N(V)) and stratification index existed in crypts between these two groups when comparing similar segments. All values in PPM were consistently lower when compared with N crypts. These preliminary observations may represent a subtly altered state of cellular differentiation in PPM which may be a reflection of early preneoplastic transformation.
Persistent Identifierhttp://hdl.handle.net/10722/67918
ISSN
2015 Impact Factor: 1.875
2015 SCImago Journal Rankings: 0.805
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTipoe, GLen_HK
dc.date.accessioned2010-09-06T05:59:27Z-
dc.date.available2010-09-06T05:59:27Z-
dc.date.issued1998en_HK
dc.identifier.citationHistology And Histopathology, 1998, v. 13 n. 2, p. 395-404en_HK
dc.identifier.issn0213-3911en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67918-
dc.description.abstractThe present study uses morphometric techniques to assess whether altered differentiation patterns exist in PPM which might reflect its premalignant status. Samples were obtained from resected malignant lesions of large bowels of 10 Chinese patients. Normal (N) samples were biopsied from the margins of each resected large bowel. Potentially premalignant (PPM) mucosae were obtained from within 2 cm of the margins of the malignant lesions. Tissues were processed for histological examination and using strict criteria, colorectal crypts were divided into basal (B), intermediate (I) and surface (S) segments. Interactive digitisation of sections from each group was used to generate the following morphometric parameters in each segment: nuclear profile circularity indices (NSF and NCI); nuclear numerical density (N(A) and N(V)); the degree of deviation of the major nuclear axis in relation to the epithelial-connective junction (AGDMAX); cell height (CH); the distance between nuclear apex to cell apex (DNACA); the distance between cell base to nuclear apex (DCBNA); stratification index (SI) - the ratio of DCBNA and CH; and the volume density of mucous vacuoles in the reference epithelium (V(VMV,EP)). In comparisons of different segments within groups, the nuclei at the S segment of N and PPM crypts were more irregular and less circular in shape than nuclei from other segments. There was a shift of nuclear profile shape (NSF and NCI) from circular to ellipsiodal between B and S segments. In comparisons of similar segments between groups, no significant nuclear shape changes were detected in nuclei of PPM crypts when compared with nuclei in similar segments of N crypts and the pattern of nuclear shape alterations resembled those of normal crypts. In comparisons of different segments within groups of N and PPM crypts, AGDMAX. DNACA, DCBNA, CH and SI parameters demonstrated that epithelial cells at the I segments have more centrally positioned nuclei with the tallest epithelial height when compared with epithelial cells in other segments of both crypts. In B segments, nuclear N(A) and N(V) were almost double those of other segments in both N and PPM crypts, with marked reductions in these parameters between B and I segments. V(VMV,EP) was significantly highest in the I segment and significantly lowest in the S segment of both groups. Both N and PPM crypts showed similar trends in V(VMV,EP) within the crypt segments but when comparing similar segments between both crypts, a significant difference was detected only between S segments. The alterations of nuclear shape and packing densities, orientation and mucous content in N crypts were similarly expressed in PPM crypts and distinct differences in numerical density (N(V)) and stratification index existed in crypts between these two groups when comparing similar segments. All values in PPM were consistently lower when compared with N crypts. These preliminary observations may represent a subtly altered state of cellular differentiation in PPM which may be a reflection of early preneoplastic transformation.en_HK
dc.languageengen_HK
dc.publisherHistology and Histopathology. The Journal's web site is located at http://www.hh.um.esen_HK
dc.relation.ispartofHistology and Histopathologyen_HK
dc.subject.meshColorectal Neoplasms - pathologyen_HK
dc.subject.meshDisease Progressionen_HK
dc.subject.meshHumansen_HK
dc.subject.meshIntestinal Mucosa - pathologyen_HK
dc.subject.meshPredictive Value of Testsen_HK
dc.titleQuantitative assessment of normal and potentially premalignant epithelium at different levels of human colorectal cryptsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0213-3911&volume=13&spage=395&epage=404&date=1998&atitle=Quantitative+assessment+of+normal+and+potentially+premalignant+epithelium+at+different+levels+of+human+colorectal+cryptsen_HK
dc.identifier.emailTipoe, GL:tgeorge@hkucc.hku.hken_HK
dc.identifier.authorityTipoe, GL=rp00371en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid9589898-
dc.identifier.scopuseid_2-s2.0-0031980754en_HK
dc.identifier.hkuros31286en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031980754&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume13en_HK
dc.identifier.issue2en_HK
dc.identifier.spage395en_HK
dc.identifier.epage404en_HK
dc.identifier.isiWOS:000073310700012-
dc.publisher.placeSpainen_HK
dc.identifier.scopusauthoridTipoe, GL=7003550610en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats