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Article: Proteomic analysis of the mode of antibacterial action of silver nanoparticles

TitleProteomic analysis of the mode of antibacterial action of silver nanoparticles
Authors
KeywordsAntibacterial agents
ATP
E. coli
Membrane potential
Outer membrane proteins
Silver ions
Silver nanoparticles
Issue Date2006
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journals/jprobs
Citation
Journal Of Proteome Research, 2006, v. 5 n. 4, p. 916-924 How to Cite?
AbstractSilver nanoparticles (nano-Ag) are potent and broad-spectrum antimicrobial agents. In this study, spherical nano-Ag (average diameter = 9.3 nm) particles were synthesized using a borohydride reduction method and the mode of their antibacterial action against E. coli was investigated by proteomic approaches (2-DE and MS identification), conducted in parallel to analyses involving solutions of Ag + ions. The proteomic data revealed that a short exposure of E. coli cells to antibacterial concentrations of nano-Ag resulted in an accumulation of envelope protein precursors, indicative of the dissipation of proton motive force. Consistent with these proteomic findings, nano-Ag were shown to destabilize the outer membrane, collapse the plasma membrane potential and deplete the levels of intracellular ATP. The mode of action of nano-Ag was also found to be similar to that of Ag + ions (e.g., Dibrov, P. et al, Antimicrob. Agents Chemother. 2002, 46, 2668-2670); however, the effective concentrations of nano-Ag and Ag + ions were at nanomolar and micromolar levels, respectively. Nano-Ag appear to be an efficient physicochemical system conferring antimicrobial silver activities. © 2006 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/67914
ISSN
2015 Impact Factor: 4.173
2015 SCImago Journal Rankings: 1.962
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLok, CNen_HK
dc.contributor.authorHo, CMen_HK
dc.contributor.authorChen, Ren_HK
dc.contributor.authorHe, QYen_HK
dc.contributor.authorYu, WYen_HK
dc.contributor.authorSun, Hen_HK
dc.contributor.authorTam, PKHen_HK
dc.contributor.authorChiu, JFen_HK
dc.contributor.authorChe, CMen_HK
dc.date.accessioned2010-09-06T05:59:25Z-
dc.date.available2010-09-06T05:59:25Z-
dc.date.issued2006en_HK
dc.identifier.citationJournal Of Proteome Research, 2006, v. 5 n. 4, p. 916-924en_HK
dc.identifier.issn1535-3893en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67914-
dc.description.abstractSilver nanoparticles (nano-Ag) are potent and broad-spectrum antimicrobial agents. In this study, spherical nano-Ag (average diameter = 9.3 nm) particles were synthesized using a borohydride reduction method and the mode of their antibacterial action against E. coli was investigated by proteomic approaches (2-DE and MS identification), conducted in parallel to analyses involving solutions of Ag + ions. The proteomic data revealed that a short exposure of E. coli cells to antibacterial concentrations of nano-Ag resulted in an accumulation of envelope protein precursors, indicative of the dissipation of proton motive force. Consistent with these proteomic findings, nano-Ag were shown to destabilize the outer membrane, collapse the plasma membrane potential and deplete the levels of intracellular ATP. The mode of action of nano-Ag was also found to be similar to that of Ag + ions (e.g., Dibrov, P. et al, Antimicrob. Agents Chemother. 2002, 46, 2668-2670); however, the effective concentrations of nano-Ag and Ag + ions were at nanomolar and micromolar levels, respectively. Nano-Ag appear to be an efficient physicochemical system conferring antimicrobial silver activities. © 2006 American Chemical Society.en_HK
dc.languageengen_HK
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journals/jprobsen_HK
dc.relation.ispartofJournal of Proteome Researchen_HK
dc.subjectAntibacterial agentsen_HK
dc.subjectATPen_HK
dc.subjectE. colien_HK
dc.subjectMembrane potentialen_HK
dc.subjectOuter membrane proteinsen_HK
dc.subjectSilver ionsen_HK
dc.subjectSilver nanoparticlesen_HK
dc.titleProteomic analysis of the mode of antibacterial action of silver nanoparticlesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1535-3893&volume=5&spage=916&epage=924&date=2006&atitle=Proteomic+analysis+of+the+mode+of+antibacterial+action+of+silver+nanoparticlesen_HK
dc.identifier.emailLok, CN: cnlok@hku.hken_HK
dc.identifier.emailHo, CM: rickyho@hkucc.hku.hken_HK
dc.identifier.emailSun, H: hsun@hku.hken_HK
dc.identifier.emailTam, PKH: paultam@hku.hken_HK
dc.identifier.emailChe, CM: cmche@hku.hken_HK
dc.identifier.authorityLok, CN=rp00752en_HK
dc.identifier.authorityHo, CM=rp00705en_HK
dc.identifier.authoritySun, H=rp00777en_HK
dc.identifier.authorityTam, PKH=rp00060en_HK
dc.identifier.authorityChe, CM=rp00670en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1021/pr0504079en_HK
dc.identifier.pmid16602699-
dc.identifier.scopuseid_2-s2.0-33645764133en_HK
dc.identifier.hkuros120640en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33645764133&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume5en_HK
dc.identifier.issue4en_HK
dc.identifier.spage916en_HK
dc.identifier.epage924en_HK
dc.identifier.isiWOS:000236816100021-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLok, CN=7006410829en_HK
dc.identifier.scopusauthoridHo, CM=12807243800en_HK
dc.identifier.scopusauthoridChen, R=9275915200en_HK
dc.identifier.scopusauthoridHe, QY=34770287900en_HK
dc.identifier.scopusauthoridYu, WY=7403913673en_HK
dc.identifier.scopusauthoridSun, H=7404827446en_HK
dc.identifier.scopusauthoridTam, PKH=7202539421en_HK
dc.identifier.scopusauthoridChiu, JF=7201501692en_HK
dc.identifier.scopusauthoridChe, CM=7102442791en_HK
dc.identifier.citeulike4028974-

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