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Article: Polysaccharides from wolfberry antagonizes glutamate excitotoxicity in rat cortical neurons

TitlePolysaccharides from wolfberry antagonizes glutamate excitotoxicity in rat cortical neurons
Authors
KeywordsC-Jun N-terminal kinase
Excitotoxicity
Glutamate
Neuroprotection
Wolfberry
Issue Date2009
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0272-4340
Citation
Cellular And Molecular Neurobiology, 2009, v. 29 n. 8, p. 1233-1244 How to Cite?
AbstractGlutamate excitotoxicity is involved in many neurodegenerative diseases including Alzheimer's disease (AD). Attenuation of glutamate toxicity is one of the therapeutic strategies for AD. Wolfberry (Lycium barbarum) is a common ingredient in oriental cuisines. A number of studies suggest that wolfberry has anti-aging properties. In recent years, there is a trend of using dried Wolfberry as food supplement and health product in UK and North America. Previously, we have demonstrated that a fraction of polysaccharide from Wolfberry (LBA) provided remarkable neuroprotective effects against beta-amyloid peptide-induced cytotoxicity in primary cultures of rat cortical neurons. To investigate whether LBA can protect neurons from other pathological factors such as glutamate found in Alzheimer brain, we examined whether it can prevent neurotoxicity elicited by glutamate in primary cultured neurons. The glutamate-induced cell death as detected by lactate dehydrogenase assay and caspase-3-like activity assay was significantly reduced by LBA at concentrations ranging from 10 to 500 μg/ml. Protective effects of LBA were comparable to memantine, a non-competitive NMDA receptor antagonist. LBA provided neuroprotection even 1 h after exposure to glutamate. In addition to glutamate, LBA attenuated N-methyl-d-aspartate (NMDA)-induced neuronal damage. To further explore whether LBA might function as antioxidant, we used hydrogen peroxide (H2O2) as oxidative stress inducer in this study. LBA could not attenuate the toxicity of H2O2. Furthermore, LBA did not attenuate glutamate-induced oxidation by using NBT assay. Western blot analysis indicated that glutamate-induced phosphorylation of c-jun N-terminal kinase (JNK) was reduced by treatment with LBA. Taken together, LBA exerted significant neuroprotective effects on cultured cortical neurons exposed to glutamate. © 2009 Springer Science+Business Media, LLC.
Persistent Identifierhttp://hdl.handle.net/10722/67913
ISSN
2015 Impact Factor: 2.328
2015 SCImago Journal Rankings: 1.005
ISI Accession Number ID
Funding AgencyGrant Number
HKU Alzheimer's Disease Research Network7552/06 M
NSFC/RGCN_HKU707/07M
HKU Seed200811159082
Azalea
Department of Chemistry
The University of Hong Kong
Funding Information:

The authors would like to thank Professor J. N. Fang for his help in providing LBA, Miss Michelle Huie for critical reading of the manuscript This work is supported by the HKU Alzheimer's Disease Research Network, General Research Grant (7552/06 M) and NSFC/RGC Joint Research Scheme (N_HKU707/07M) from Research Grant Council, and HKU Seed Funding for Basic Research (200811159082) to RCCC. Also, the work is supported by Azalea (1972) Endowment Fund. WHY would like to thank for the support from the Department of Chemistry. YSH is supported by the Graduate School, MSY is supported by Postdoctoral Fellowship, The University of Hong Kong.

References

 

DC FieldValueLanguage
dc.contributor.authorHo, YSen_HK
dc.contributor.authorYu, MSen_HK
dc.contributor.authorYik, SYen_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorYuen, WHen_HK
dc.contributor.authorChang, RCCen_HK
dc.date.accessioned2010-09-06T05:59:24Z-
dc.date.available2010-09-06T05:59:24Z-
dc.date.issued2009en_HK
dc.identifier.citationCellular And Molecular Neurobiology, 2009, v. 29 n. 8, p. 1233-1244en_HK
dc.identifier.issn0272-4340en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67913-
dc.description.abstractGlutamate excitotoxicity is involved in many neurodegenerative diseases including Alzheimer's disease (AD). Attenuation of glutamate toxicity is one of the therapeutic strategies for AD. Wolfberry (Lycium barbarum) is a common ingredient in oriental cuisines. A number of studies suggest that wolfberry has anti-aging properties. In recent years, there is a trend of using dried Wolfberry as food supplement and health product in UK and North America. Previously, we have demonstrated that a fraction of polysaccharide from Wolfberry (LBA) provided remarkable neuroprotective effects against beta-amyloid peptide-induced cytotoxicity in primary cultures of rat cortical neurons. To investigate whether LBA can protect neurons from other pathological factors such as glutamate found in Alzheimer brain, we examined whether it can prevent neurotoxicity elicited by glutamate in primary cultured neurons. The glutamate-induced cell death as detected by lactate dehydrogenase assay and caspase-3-like activity assay was significantly reduced by LBA at concentrations ranging from 10 to 500 μg/ml. Protective effects of LBA were comparable to memantine, a non-competitive NMDA receptor antagonist. LBA provided neuroprotection even 1 h after exposure to glutamate. In addition to glutamate, LBA attenuated N-methyl-d-aspartate (NMDA)-induced neuronal damage. To further explore whether LBA might function as antioxidant, we used hydrogen peroxide (H2O2) as oxidative stress inducer in this study. LBA could not attenuate the toxicity of H2O2. Furthermore, LBA did not attenuate glutamate-induced oxidation by using NBT assay. Western blot analysis indicated that glutamate-induced phosphorylation of c-jun N-terminal kinase (JNK) was reduced by treatment with LBA. Taken together, LBA exerted significant neuroprotective effects on cultured cortical neurons exposed to glutamate. © 2009 Springer Science+Business Media, LLC.en_HK
dc.languageengen_HK
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0272-4340en_HK
dc.relation.ispartofCellular and Molecular Neurobiologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsThe original publication is available at www.springerlink.com-
dc.subjectC-Jun N-terminal kinaseen_HK
dc.subjectExcitotoxicityen_HK
dc.subjectGlutamateen_HK
dc.subjectNeuroprotectionen_HK
dc.subjectWolfberryen_HK
dc.subject.meshCerebral Cortex - pathology-
dc.subject.meshGlutamic Acid - toxicity-
dc.subject.meshLycium - chemistry-
dc.subject.meshNeurons - drug effects - enzymology - pathology-
dc.subject.meshNeurotoxins - toxicity-
dc.titlePolysaccharides from wolfberry antagonizes glutamate excitotoxicity in rat cortical neuronsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0272-4340&volume=29&issue=8&spage=1233&epage=1244&date=2009&atitle=Polysaccharides+from+wolfberry+antagonizes+glutamate+excitotoxicity+in+rat+cortical+neuronsen_HK
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_HK
dc.identifier.emailChang, RCC:rccchang@hkucc.hku.hken_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.identifier.authorityChang, RCC=rp00470en_HK
dc.description.naturepostprint-
dc.identifier.doi10.1007/s10571-009-9419-xen_HK
dc.identifier.pmid19499323-
dc.identifier.scopuseid_2-s2.0-77049085291en_HK
dc.identifier.hkuros169235en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77049085291&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume29en_HK
dc.identifier.issue8en_HK
dc.identifier.spage1233en_HK
dc.identifier.epage1244en_HK
dc.identifier.isiWOS:000272781000018-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHo, YS=14031513600en_HK
dc.identifier.scopusauthoridYu, MS=35346047600en_HK
dc.identifier.scopusauthoridYik, SY=35520460000en_HK
dc.identifier.scopusauthoridSo, KF=34668391300en_HK
dc.identifier.scopusauthoridYuen, WH=7102761282en_HK
dc.identifier.scopusauthoridChang, RCC=7403713410en_HK
dc.identifier.citeulike4846734-

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