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Article: Downregulation of hemidesmosomal proteins in nasopharyngeal carcinoma cells

TitleDownregulation of hemidesmosomal proteins in nasopharyngeal carcinoma cells
Authors
Issue Date2001
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/canlet
Citation
Cancer Letters, 2001, v. 163 n. 1, p. 117-124 How to Cite?
Abstract
Hemidesmosome (HD) is a transmembrane complex that mediates attachment of epithelial cells to the basement membrane. Abnormal expression of HD components has been reported in several types of human cancers and is believed to play a role in tumor invasion and metastasis. Using differential gene display, we have identified downregulation of BPAG1 expression in nasopharyngeal carcinoma cells. BPAG1 is a major component of hemidesmosome. In the present study, we have extended our work to investigate the expression pattern of other components in the HD complex, namely, BPAG2, ITGα6 and ITGβ4 in three distinct biological groups of nasopharyngeal epithelial cells: (a) non-malignant nasopharyngeal epithelial cells established from primary culture of nasopharyngeal explants, (b) non-malignant nasopharyngeal epithelial cells immortalized by viral oncogenes, SV40 or HPV16E6E7, and (c) nasopharyngeal carcinoma (NPC) cells. Both non-malignant primary cultured nasopharyngeal epithelial cells and immortalized nasopharyngeal epithelial cell lines expressed all the HD components examined, although the immortalized cells expressed a lower level of HD components compared with the non-malignant nasopharyngeal cells established from primary culture. In contrast, downregulation of HD components is commonly observed in nasopharyngeal carcinoma cells. Loss of HD expression in NPC may be associated with the undifferentiated properties of NPC cells and may have prognostic significance. © 2001 Elsevier Science Ireland Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/67909
ISSN
2013 Impact Factor: 5.016
ISI Accession Number ID
References

 

Author Affiliations
  1. The University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorLo, AKFen_HK
dc.contributor.authorYuen, PWen_HK
dc.contributor.authorLiu, Yen_HK
dc.contributor.authorWang, XHen_HK
dc.contributor.authorCheung, ALMen_HK
dc.contributor.authorWong, YCen_HK
dc.contributor.authorTsao, SWen_HK
dc.date.accessioned2010-09-06T05:59:22Z-
dc.date.available2010-09-06T05:59:22Z-
dc.date.issued2001en_HK
dc.identifier.citationCancer Letters, 2001, v. 163 n. 1, p. 117-124en_HK
dc.identifier.issn0304-3835en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67909-
dc.description.abstractHemidesmosome (HD) is a transmembrane complex that mediates attachment of epithelial cells to the basement membrane. Abnormal expression of HD components has been reported in several types of human cancers and is believed to play a role in tumor invasion and metastasis. Using differential gene display, we have identified downregulation of BPAG1 expression in nasopharyngeal carcinoma cells. BPAG1 is a major component of hemidesmosome. In the present study, we have extended our work to investigate the expression pattern of other components in the HD complex, namely, BPAG2, ITGα6 and ITGβ4 in three distinct biological groups of nasopharyngeal epithelial cells: (a) non-malignant nasopharyngeal epithelial cells established from primary culture of nasopharyngeal explants, (b) non-malignant nasopharyngeal epithelial cells immortalized by viral oncogenes, SV40 or HPV16E6E7, and (c) nasopharyngeal carcinoma (NPC) cells. Both non-malignant primary cultured nasopharyngeal epithelial cells and immortalized nasopharyngeal epithelial cell lines expressed all the HD components examined, although the immortalized cells expressed a lower level of HD components compared with the non-malignant nasopharyngeal cells established from primary culture. In contrast, downregulation of HD components is commonly observed in nasopharyngeal carcinoma cells. Loss of HD expression in NPC may be associated with the undifferentiated properties of NPC cells and may have prognostic significance. © 2001 Elsevier Science Ireland Ltd.en_HK
dc.languageengen_HK
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/canleten_HK
dc.relation.ispartofCancer Lettersen_HK
dc.rightsCancer Letters. Copyright © Elsevier Ireland Ltd.en_HK
dc.subject.meshAntigens, CD - genetics - metabolismen_HK
dc.subject.meshAutoantigens - genetics - metabolismen_HK
dc.subject.meshBlotting, Westernen_HK
dc.subject.meshCarrier Proteinsen_HK
dc.subject.meshCell Line, Transformeden_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshCollagen - genetics - metabolismen_HK
dc.subject.meshCytoskeletal Proteinsen_HK
dc.subject.meshDown-Regulationen_HK
dc.subject.meshGene Expression Regulation, Neoplasticen_HK
dc.subject.meshHemidesmosomes - genetics - metabolismen_HK
dc.subject.meshHumansen_HK
dc.subject.meshIntegrin alpha6en_HK
dc.subject.meshIntegrin beta4en_HK
dc.subject.meshIntegrins - genetics - metabolismen_HK
dc.subject.meshNasopharyngeal Neoplasms - genetics - metabolism - pathologyen_HK
dc.subject.meshNerve Tissue Proteinsen_HK
dc.subject.meshNon-Fibrillar Collagensen_HK
dc.subject.meshRNA, Messenger - genetics - metabolismen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshTumor Cells, Cultureden_HK
dc.titleDownregulation of hemidesmosomal proteins in nasopharyngeal carcinoma cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0304-3835&volume=163&spage=117&epage=124&date=2001&atitle=Downregulation+of+hemidesmosomal+proteins+in+nasopharyngeal+carcinoma+cellsen_HK
dc.identifier.emailCheung, ALM:lmcheung@hkucc.hku.hken_HK
dc.identifier.emailWong, YC:ycwong@hkucc.hku.hken_HK
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_HK
dc.identifier.authorityCheung, ALM=rp00332en_HK
dc.identifier.authorityWong, YC=rp00316en_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0304-3835(00)00683-2en_HK
dc.identifier.pmid11163115en_HK
dc.identifier.scopuseid_2-s2.0-0035835496en_HK
dc.identifier.hkuros63763en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035835496&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume163en_HK
dc.identifier.issue1en_HK
dc.identifier.spage117en_HK
dc.identifier.epage124en_HK
dc.identifier.isiWOS:000168440500015-
dc.publisher.placeIrelanden_HK
dc.identifier.scopusauthoridLo, AKF=7102780657en_HK
dc.identifier.scopusauthoridYuen, PW=7103124007en_HK
dc.identifier.scopusauthoridLiu, Y=26643293600en_HK
dc.identifier.scopusauthoridWang, XH=7501854829en_HK
dc.identifier.scopusauthoridCheung, ALM=7401806497en_HK
dc.identifier.scopusauthoridWong, YC=7403041798en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK

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