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Article: Downregulation of hemidesmosomal proteins in nasopharyngeal carcinoma cells
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TitleDownregulation of hemidesmosomal proteins in nasopharyngeal carcinoma cells
 
AuthorsLo, AKF1
Yuen, PW1
Liu, Y1
Wang, XH1
Cheung, ALM1
Wong, YC1
Tsao, SW1
 
Issue Date2001
 
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/canlet
 
CitationCancer Letters, 2001, v. 163 n. 1, p. 117-124 [How to Cite?]
DOI: http://dx.doi.org/10.1016/S0304-3835(00)00683-2
 
AbstractHemidesmosome (HD) is a transmembrane complex that mediates attachment of epithelial cells to the basement membrane. Abnormal expression of HD components has been reported in several types of human cancers and is believed to play a role in tumor invasion and metastasis. Using differential gene display, we have identified downregulation of BPAG1 expression in nasopharyngeal carcinoma cells. BPAG1 is a major component of hemidesmosome. In the present study, we have extended our work to investigate the expression pattern of other components in the HD complex, namely, BPAG2, ITGα6 and ITGβ4 in three distinct biological groups of nasopharyngeal epithelial cells: (a) non-malignant nasopharyngeal epithelial cells established from primary culture of nasopharyngeal explants, (b) non-malignant nasopharyngeal epithelial cells immortalized by viral oncogenes, SV40 or HPV16E6E7, and (c) nasopharyngeal carcinoma (NPC) cells. Both non-malignant primary cultured nasopharyngeal epithelial cells and immortalized nasopharyngeal epithelial cell lines expressed all the HD components examined, although the immortalized cells expressed a lower level of HD components compared with the non-malignant nasopharyngeal cells established from primary culture. In contrast, downregulation of HD components is commonly observed in nasopharyngeal carcinoma cells. Loss of HD expression in NPC may be associated with the undifferentiated properties of NPC cells and may have prognostic significance. © 2001 Elsevier Science Ireland Ltd.
 
ISSN0304-3835
2012 Impact Factor: 4.258
2012 SCImago Journal Rankings: 1.502
 
DOIhttp://dx.doi.org/10.1016/S0304-3835(00)00683-2
 
ISI Accession Number IDWOS:000168440500015
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorLo, AKF
 
dc.contributor.authorYuen, PW
 
dc.contributor.authorLiu, Y
 
dc.contributor.authorWang, XH
 
dc.contributor.authorCheung, ALM
 
dc.contributor.authorWong, YC
 
dc.contributor.authorTsao, SW
 
dc.date.accessioned2010-09-06T05:59:22Z
 
dc.date.available2010-09-06T05:59:22Z
 
dc.date.issued2001
 
dc.description.abstractHemidesmosome (HD) is a transmembrane complex that mediates attachment of epithelial cells to the basement membrane. Abnormal expression of HD components has been reported in several types of human cancers and is believed to play a role in tumor invasion and metastasis. Using differential gene display, we have identified downregulation of BPAG1 expression in nasopharyngeal carcinoma cells. BPAG1 is a major component of hemidesmosome. In the present study, we have extended our work to investigate the expression pattern of other components in the HD complex, namely, BPAG2, ITGα6 and ITGβ4 in three distinct biological groups of nasopharyngeal epithelial cells: (a) non-malignant nasopharyngeal epithelial cells established from primary culture of nasopharyngeal explants, (b) non-malignant nasopharyngeal epithelial cells immortalized by viral oncogenes, SV40 or HPV16E6E7, and (c) nasopharyngeal carcinoma (NPC) cells. Both non-malignant primary cultured nasopharyngeal epithelial cells and immortalized nasopharyngeal epithelial cell lines expressed all the HD components examined, although the immortalized cells expressed a lower level of HD components compared with the non-malignant nasopharyngeal cells established from primary culture. In contrast, downregulation of HD components is commonly observed in nasopharyngeal carcinoma cells. Loss of HD expression in NPC may be associated with the undifferentiated properties of NPC cells and may have prognostic significance. © 2001 Elsevier Science Ireland Ltd.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationCancer Letters, 2001, v. 163 n. 1, p. 117-124 [How to Cite?]
DOI: http://dx.doi.org/10.1016/S0304-3835(00)00683-2
 
dc.identifier.doihttp://dx.doi.org/10.1016/S0304-3835(00)00683-2
 
dc.identifier.epage124
 
dc.identifier.hkuros63763
 
dc.identifier.isiWOS:000168440500015
 
dc.identifier.issn0304-3835
2012 Impact Factor: 4.258
2012 SCImago Journal Rankings: 1.502
 
dc.identifier.issue1
 
dc.identifier.openurl
 
dc.identifier.pmid11163115
 
dc.identifier.scopuseid_2-s2.0-0035835496
 
dc.identifier.spage117
 
dc.identifier.urihttp://hdl.handle.net/10722/67909
 
dc.identifier.volume163
 
dc.languageeng
 
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/canlet
 
dc.publisher.placeIreland
 
dc.relation.ispartofCancer Letters
 
dc.relation.referencesReferences in Scopus
 
dc.rightsCancer Letters. Copyright © Elsevier Ireland Ltd.
 
dc.subject.meshAntigens, CD - genetics - metabolism
 
dc.subject.meshAutoantigens - genetics - metabolism
 
dc.subject.meshBlotting, Western
 
dc.subject.meshCarrier Proteins
 
dc.subject.meshCell Line, Transformed
 
dc.subject.meshCells, Cultured
 
dc.subject.meshCollagen - genetics - metabolism
 
dc.subject.meshCytoskeletal Proteins
 
dc.subject.meshDown-Regulation
 
dc.subject.meshGene Expression Regulation, Neoplastic
 
dc.subject.meshHemidesmosomes - genetics - metabolism
 
dc.subject.meshHumans
 
dc.subject.meshIntegrin alpha6
 
dc.subject.meshIntegrin beta4
 
dc.subject.meshIntegrins - genetics - metabolism
 
dc.subject.meshNasopharyngeal Neoplasms - genetics - metabolism - pathology
 
dc.subject.meshNerve Tissue Proteins
 
dc.subject.meshNon-Fibrillar Collagens
 
dc.subject.meshRNA, Messenger - genetics - metabolism
 
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction
 
dc.subject.meshTumor Cells, Cultured
 
dc.titleDownregulation of hemidesmosomal proteins in nasopharyngeal carcinoma cells
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong